Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cybC and nrdG are separated by 44 nucleotides nrdG and nrdD are separated by 157 nucleotides nrdD and treC are separated by 393 nucleotides
b4236: cybC - cytochrome b(562) (VIMSS), at 4,457,513 to 4,457,878
cybC
b4237: nrdG - anaerobic ribonucleotide reductase activating protein (NCBI), at 4,457,923 to 4,458,387
nrdG
b4238: nrdD - anaerobic ribonucleoside triphosphate reductase (NCBI), at 4,458,545 to 4,460,683
nrdD
b4239: treC - trehalose-6-P hydrolase (NCBI), at 4,461,077 to 4,462,732
treC
Position (kb)
4458
4459
4460
4461 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4457.572 kb on + strand, within cybC at 4457.632 kb on + strand, within cybC at 4457.632 kb on + strand, within cybC at 4457.653 kb on + strand, within cybC at 4457.711 kb on + strand, within cybC at 4457.788 kb on - strand, within cybC at 4457.788 kb on - strand, within cybC at 4457.806 kb on + strand, within cybC at 4457.806 kb on + strand, within cybC at 4457.831 kb on - strand, within cybC at 4457.831 kb on - strand, within cybC at 4457.834 kb on - strand, within cybC at 4457.853 kb on + strand at 4457.853 kb on + strand at 4457.853 kb on + strand at 4457.903 kb on - strand at 4457.944 kb on - strand at 4457.944 kb on - strand at 4458.063 kb on + strand, within nrdG at 4458.074 kb on + strand, within nrdG at 4458.095 kb on + strand, within nrdG at 4458.095 kb on + strand, within nrdG at 4458.126 kb on - strand, within nrdG at 4458.126 kb on - strand, within nrdG at 4458.154 kb on + strand, within nrdG at 4458.179 kb on + strand, within nrdG at 4458.179 kb on + strand, within nrdG at 4458.191 kb on + strand, within nrdG at 4458.191 kb on + strand, within nrdG at 4458.229 kb on - strand, within nrdG at 4458.233 kb on + strand, within nrdG at 4458.241 kb on - strand, within nrdG at 4458.241 kb on - strand, within nrdG at 4458.241 kb on - strand, within nrdG at 4458.247 kb on - strand, within nrdG at 4458.247 kb on - strand, within nrdG at 4458.264 kb on + strand, within nrdG at 4458.294 kb on - strand, within nrdG at 4458.294 kb on - strand, within nrdG at 4458.301 kb on + strand, within nrdG at 4458.354 kb on - strand at 4458.365 kb on + strand at 4458.365 kb on + strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.432 kb on + strand at 4458.479 kb on + strand at 4458.479 kb on + strand at 4458.489 kb on - strand at 4458.494 kb on - strand at 4458.571 kb on - strand at 4458.571 kb on - strand at 4458.591 kb on + strand at 4458.613 kb on + strand at 4458.630 kb on - strand at 4458.630 kb on - strand at 4458.677 kb on + strand at 4458.677 kb on + strand at 4458.677 kb on - strand at 4458.698 kb on - strand at 4458.709 kb on + strand at 4458.763 kb on - strand, within nrdD at 4458.768 kb on + strand, within nrdD at 4458.779 kb on + strand, within nrdD at 4458.935 kb on - strand, within nrdD at 4458.971 kb on - strand, within nrdD at 4458.994 kb on - strand, within nrdD at 4458.994 kb on - strand, within nrdD at 4459.004 kb on - strand, within nrdD at 4459.011 kb on + strand, within nrdD at 4459.019 kb on - strand, within nrdD at 4459.019 kb on - strand, within nrdD at 4459.053 kb on + strand, within nrdD at 4459.053 kb on + strand, within nrdD at 4459.063 kb on - strand, within nrdD at 4459.063 kb on - strand, within nrdD at 4459.066 kb on - strand, within nrdD at 4459.091 kb on + strand, within nrdD at 4459.130 kb on - strand, within nrdD at 4459.157 kb on + strand, within nrdD at 4459.158 kb on - strand, within nrdD at 4459.173 kb on + strand, within nrdD at 4459.202 kb on - strand, within nrdD at 4459.269 kb on + strand, within nrdD at 4459.269 kb on + strand, within nrdD at 4459.282 kb on + strand, within nrdD at 4459.282 kb on + strand, within nrdD at 4459.285 kb on + strand, within nrdD at 4459.285 kb on + strand, within nrdD at 4459.307 kb on + strand, within nrdD at 4459.307 kb on - strand, within nrdD at 4459.332 kb on + strand, within nrdD at 4459.332 kb on + strand, within nrdD at 4459.334 kb on + strand, within nrdD at 4459.352 kb on + strand, within nrdD at 4459.447 kb on + strand, within nrdD at 4459.447 kb on + strand, within nrdD at 4459.455 kb on - strand, within nrdD at 4459.455 kb on - strand, within nrdD at 4459.455 kb on - strand, within nrdD at 4459.455 kb on - strand, within nrdD at 4459.627 kb on - strand, within nrdD at 4459.743 kb on + strand, within nrdD at 4459.751 kb on - strand, within nrdD at 4459.751 kb on - strand, within nrdD at 4459.809 kb on + strand, within nrdD at 4459.886 kb on - strand, within nrdD at 4459.888 kb on + strand, within nrdD at 4459.896 kb on + strand, within nrdD at 4459.913 kb on + strand, within nrdD at 4459.922 kb on + strand, within nrdD at 4459.929 kb on + strand, within nrdD at 4459.937 kb on + strand, within nrdD at 4459.942 kb on - strand, within nrdD at 4459.942 kb on - strand, within nrdD at 4459.947 kb on + strand, within nrdD at 4459.949 kb on - strand, within nrdD at 4459.957 kb on - strand, within nrdD at 4459.982 kb on - strand, within nrdD at 4460.013 kb on - strand, within nrdD at 4460.029 kb on - strand, within nrdD at 4460.029 kb on - strand, within nrdD at 4460.061 kb on + strand, within nrdD at 4460.069 kb on - strand, within nrdD at 4460.076 kb on + strand, within nrdD at 4460.076 kb on + strand, within nrdD at 4460.100 kb on + strand, within nrdD at 4460.168 kb on - strand, within nrdD at 4460.186 kb on + strand, within nrdD at 4460.196 kb on + strand, within nrdD at 4460.196 kb on + strand, within nrdD at 4460.218 kb on + strand, within nrdD at 4460.225 kb on + strand, within nrdD at 4460.225 kb on + strand, within nrdD at 4460.238 kb on - strand, within nrdD at 4460.243 kb on + strand, within nrdD at 4460.260 kb on - strand, within nrdD at 4460.360 kb on - strand, within nrdD at 4460.371 kb on + strand, within nrdD at 4460.371 kb on + strand, within nrdD at 4460.387 kb on + strand, within nrdD at 4460.447 kb on - strand, within nrdD at 4460.447 kb on - strand, within nrdD at 4460.504 kb on - strand at 4460.504 kb on - strand at 4460.542 kb on - strand at 4460.547 kb on - strand at 4460.554 kb on + strand at 4460.661 kb on + strand at 4460.820 kb on + strand at 4460.876 kb on + strand at 4460.891 kb on - strand at 4460.891 kb on - strand at 4461.038 kb on + strand at 4461.038 kb on + strand at 4461.080 kb on + strand at 4461.097 kb on - strand at 4461.128 kb on + strand at 4461.145 kb on + strand at 4461.190 kb on - strand at 4461.295 kb on + strand, within treC at 4461.314 kb on + strand, within treC at 4461.427 kb on + strand, within treC at 4461.533 kb on + strand, within treC at 4461.533 kb on + strand, within treC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 4,457,572 + cybC b4236 0.16 -0.6 4,457,632 + cybC b4236 0.33 -0.5 4,457,632 + cybC b4236 0.33 -0.8 4,457,653 + cybC b4236 0.38 -0.1 4,457,711 + cybC b4236 0.54 -0.1 4,457,788 - cybC b4236 0.75 -0.4 4,457,788 - cybC b4236 0.75 -1.2 4,457,806 + cybC b4236 0.80 -0.2 4,457,806 + cybC b4236 0.80 +1.2 4,457,831 - cybC b4236 0.87 -0.2 4,457,831 - cybC b4236 0.87 -0.1 4,457,834 - cybC b4236 0.88 -0.2 4,457,853 + +0.1 4,457,853 + -1.2 4,457,853 + +0.2 4,457,903 - -0.4 4,457,944 - -0.8 4,457,944 - -0.0 4,458,063 + nrdG b4237 0.30 -1.3 4,458,074 + nrdG b4237 0.32 -1.8 4,458,095 + nrdG b4237 0.37 -1.7 4,458,095 + nrdG b4237 0.37 -1.3 4,458,126 - nrdG b4237 0.44 -2.0 4,458,126 - nrdG b4237 0.44 -2.3 4,458,154 + nrdG b4237 0.50 -1.6 4,458,179 + nrdG b4237 0.55 -1.2 4,458,179 + nrdG b4237 0.55 -0.7 4,458,191 + nrdG b4237 0.58 -1.7 4,458,191 + nrdG b4237 0.58 -1.3 4,458,229 - nrdG b4237 0.66 -1.6 4,458,233 + nrdG b4237 0.67 -1.6 4,458,241 - nrdG b4237 0.68 -1.0 4,458,241 - nrdG b4237 0.68 -1.9 4,458,241 - nrdG b4237 0.68 -1.8 4,458,247 - nrdG b4237 0.70 -1.4 4,458,247 - nrdG b4237 0.70 -2.0 4,458,264 + nrdG b4237 0.73 -1.4 4,458,294 - nrdG b4237 0.80 -0.4 4,458,294 - nrdG b4237 0.80 -0.6 4,458,301 + nrdG b4237 0.81 -0.9 4,458,354 - -2.0 4,458,365 + -0.9 4,458,365 + -1.4 4,458,373 - -0.3 4,458,373 - -1.1 4,458,373 - -2.8 4,458,373 - -2.1 4,458,373 - -0.4 4,458,373 - -0.4 4,458,432 + -0.4 4,458,479 + +0.1 4,458,479 + -2.3 4,458,489 - -0.2 4,458,494 - -0.3 4,458,571 - -0.9 4,458,571 - -1.2 4,458,591 + +0.5 4,458,613 + -0.8 4,458,630 - -1.6 4,458,630 - -2.7 4,458,677 + -0.1 4,458,677 + -1.2 4,458,677 - -0.7 4,458,698 - -0.9 4,458,709 + -2.8 4,458,763 - nrdD b4238 0.10 -0.7 4,458,768 + nrdD b4238 0.10 -1.6 4,458,779 + nrdD b4238 0.11 -1.6 4,458,935 - nrdD b4238 0.18 -0.3 4,458,971 - nrdD b4238 0.20 -1.2 4,458,994 - nrdD b4238 0.21 -1.0 4,458,994 - nrdD b4238 0.21 -1.1 4,459,004 - nrdD b4238 0.21 -1.9 4,459,011 + nrdD b4238 0.22 -0.8 4,459,019 - nrdD b4238 0.22 -1.9 4,459,019 - nrdD b4238 0.22 -0.9 4,459,053 + nrdD b4238 0.24 -1.7 4,459,053 + nrdD b4238 0.24 -1.0 4,459,063 - nrdD b4238 0.24 -1.2 4,459,063 - nrdD b4238 0.24 -0.7 4,459,066 - nrdD b4238 0.24 -0.7 4,459,091 + nrdD b4238 0.26 -0.9 4,459,130 - nrdD b4238 0.27 -2.7 4,459,157 + nrdD b4238 0.29 -1.3 4,459,158 - nrdD b4238 0.29 -1.7 4,459,173 + nrdD b4238 0.29 -1.3 4,459,202 - nrdD b4238 0.31 +0.9 4,459,269 + nrdD b4238 0.34 -1.0 4,459,269 + nrdD b4238 0.34 -1.5 4,459,282 + nrdD b4238 0.34 -0.2 4,459,282 + nrdD b4238 0.34 -1.6 4,459,285 + nrdD b4238 0.35 -1.9 4,459,285 + nrdD b4238 0.35 -1.5 4,459,307 + nrdD b4238 0.36 -0.9 4,459,307 - nrdD b4238 0.36 -1.3 4,459,332 + nrdD b4238 0.37 -2.8 4,459,332 + nrdD b4238 0.37 -2.0 4,459,334 + nrdD b4238 0.37 -0.9 4,459,352 + nrdD b4238 0.38 -2.0 4,459,447 + nrdD b4238 0.42 -0.7 4,459,447 + nrdD b4238 0.42 -1.4 4,459,455 - nrdD b4238 0.43 -0.6 4,459,455 - nrdD b4238 0.43 -1.1 4,459,455 - nrdD b4238 0.43 -2.2 4,459,455 - nrdD b4238 0.43 -1.3 4,459,627 - nrdD b4238 0.51 -1.1 4,459,743 + nrdD b4238 0.56 -1.7 4,459,751 - nrdD b4238 0.56 -1.0 4,459,751 - nrdD b4238 0.56 -2.2 4,459,809 + nrdD b4238 0.59 -1.0 4,459,886 - nrdD b4238 0.63 -0.8 4,459,888 + nrdD b4238 0.63 +0.1 4,459,896 + nrdD b4238 0.63 -1.6 4,459,913 + nrdD b4238 0.64 -2.0 4,459,922 + nrdD b4238 0.64 -0.1 4,459,929 + nrdD b4238 0.65 -0.6 4,459,937 + nrdD b4238 0.65 +0.8 4,459,942 - nrdD b4238 0.65 -1.0 4,459,942 - nrdD b4238 0.65 -0.0 4,459,947 + nrdD b4238 0.66 -1.6 4,459,949 - nrdD b4238 0.66 -0.2 4,459,957 - nrdD b4238 0.66 -0.7 4,459,982 - nrdD b4238 0.67 -2.3 4,460,013 - nrdD b4238 0.69 -1.3 4,460,029 - nrdD b4238 0.69 -1.4 4,460,029 - nrdD b4238 0.69 -2.3 4,460,061 + nrdD b4238 0.71 -0.8 4,460,069 - nrdD b4238 0.71 -1.4 4,460,076 + nrdD b4238 0.72 -0.8 4,460,076 + nrdD b4238 0.72 -1.1 4,460,100 + nrdD b4238 0.73 -1.6 4,460,168 - nrdD b4238 0.76 -1.7 4,460,186 + nrdD b4238 0.77 -1.0 4,460,196 + nrdD b4238 0.77 -2.0 4,460,196 + nrdD b4238 0.77 -0.9 4,460,218 + nrdD b4238 0.78 -2.5 4,460,225 + nrdD b4238 0.79 -0.2 4,460,225 + nrdD b4238 0.79 -0.3 4,460,238 - nrdD b4238 0.79 -0.6 4,460,243 + nrdD b4238 0.79 -0.3 4,460,260 - nrdD b4238 0.80 -0.7 4,460,360 - nrdD b4238 0.85 -1.3 4,460,371 + nrdD b4238 0.85 -0.5 4,460,371 + nrdD b4238 0.85 -0.8 4,460,387 + nrdD b4238 0.86 -1.1 4,460,447 - nrdD b4238 0.89 -1.1 4,460,447 - nrdD b4238 0.89 -1.0 4,460,504 - -0.3 4,460,504 - -1.9 4,460,542 - +0.4 4,460,547 - -0.3 4,460,554 + -0.3 4,460,661 + -1.7 4,460,820 + -1.0 4,460,876 + -0.7 4,460,891 - +1.1 4,460,891 - -0.7 4,461,038 + +1.3 4,461,038 + -0.3 4,461,080 + +0.8 4,461,097 - +0.8 4,461,128 + +0.2 4,461,145 + +0.2 4,461,190 - +1.8 4,461,295 + treC b4239 0.13 +0.2 4,461,314 + treC b4239 0.14 +0.8 4,461,427 + treC b4239 0.21 +1.8 4,461,533 + treC b4239 0.28 +0.2 4,461,533 + treC b4239 0.28 +0.8
Or see this region's nucleotide sequence