Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pmbA and cybC are separated by 178 nucleotides cybC and nrdG are separated by 44 nucleotides nrdG and nrdD are separated by 157 nucleotides
b4235: pmbA - predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI), at 4,455,982 to 4,457,334
pmbA
b4236: cybC - cytochrome b(562) (VIMSS), at 4,457,513 to 4,457,878
cybC
b4237: nrdG - anaerobic ribonucleotide reductase activating protein (NCBI), at 4,457,923 to 4,458,387
nrdG
b4238: nrdD - anaerobic ribonucleoside triphosphate reductase (NCBI), at 4,458,545 to 4,460,683
nrdD
Position (kb)
4457
4458
4459 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4456.966 kb on - strand, within pmbA at 4457.011 kb on - strand, within pmbA at 4457.029 kb on - strand, within pmbA at 4457.050 kb on + strand, within pmbA at 4457.077 kb on + strand, within pmbA at 4457.079 kb on - strand, within pmbA at 4457.090 kb on + strand, within pmbA at 4457.171 kb on - strand, within pmbA at 4457.171 kb on - strand, within pmbA at 4457.279 kb on + strand at 4457.279 kb on + strand at 4457.279 kb on + strand at 4457.281 kb on + strand at 4457.287 kb on - strand at 4457.287 kb on - strand at 4457.287 kb on - strand at 4457.294 kb on + strand at 4457.312 kb on - strand at 4457.433 kb on - strand at 4457.438 kb on + strand at 4457.479 kb on + strand at 4457.487 kb on - strand at 4457.487 kb on - strand at 4457.530 kb on - strand at 4457.539 kb on - strand at 4457.572 kb on + strand, within cybC at 4457.632 kb on + strand, within cybC at 4457.632 kb on + strand, within cybC at 4457.653 kb on + strand, within cybC at 4457.711 kb on + strand, within cybC at 4457.788 kb on - strand, within cybC at 4457.788 kb on - strand, within cybC at 4457.806 kb on + strand, within cybC at 4457.806 kb on + strand, within cybC at 4457.831 kb on - strand, within cybC at 4457.831 kb on - strand, within cybC at 4457.834 kb on - strand, within cybC at 4457.853 kb on + strand at 4457.853 kb on + strand at 4457.853 kb on + strand at 4457.903 kb on - strand at 4457.944 kb on - strand at 4457.944 kb on - strand at 4458.063 kb on + strand, within nrdG at 4458.074 kb on + strand, within nrdG at 4458.095 kb on + strand, within nrdG at 4458.095 kb on + strand, within nrdG at 4458.126 kb on - strand, within nrdG at 4458.126 kb on - strand, within nrdG at 4458.154 kb on + strand, within nrdG at 4458.179 kb on + strand, within nrdG at 4458.179 kb on + strand, within nrdG at 4458.191 kb on + strand, within nrdG at 4458.191 kb on + strand, within nrdG at 4458.229 kb on - strand, within nrdG at 4458.233 kb on + strand, within nrdG at 4458.241 kb on - strand, within nrdG at 4458.241 kb on - strand, within nrdG at 4458.241 kb on - strand, within nrdG at 4458.247 kb on - strand, within nrdG at 4458.247 kb on - strand, within nrdG at 4458.264 kb on + strand, within nrdG at 4458.294 kb on - strand, within nrdG at 4458.294 kb on - strand, within nrdG at 4458.301 kb on + strand, within nrdG at 4458.354 kb on - strand at 4458.365 kb on + strand at 4458.365 kb on + strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.373 kb on - strand at 4458.432 kb on + strand at 4458.479 kb on + strand at 4458.479 kb on + strand at 4458.489 kb on - strand at 4458.494 kb on - strand at 4458.571 kb on - strand at 4458.571 kb on - strand at 4458.591 kb on + strand at 4458.613 kb on + strand at 4458.630 kb on - strand at 4458.630 kb on - strand at 4458.677 kb on + strand at 4458.677 kb on + strand at 4458.677 kb on - strand at 4458.698 kb on - strand at 4458.709 kb on + strand at 4458.763 kb on - strand, within nrdD at 4458.768 kb on + strand, within nrdD at 4458.779 kb on + strand, within nrdD at 4458.935 kb on - strand, within nrdD at 4458.971 kb on - strand, within nrdD at 4458.994 kb on - strand, within nrdD at 4458.994 kb on - strand, within nrdD at 4459.004 kb on - strand, within nrdD at 4459.011 kb on + strand, within nrdD at 4459.019 kb on - strand, within nrdD at 4459.019 kb on - strand, within nrdD at 4459.053 kb on + strand, within nrdD at 4459.053 kb on + strand, within nrdD at 4459.063 kb on - strand, within nrdD at 4459.063 kb on - strand, within nrdD at 4459.066 kb on - strand, within nrdD at 4459.091 kb on + strand, within nrdD at 4459.130 kb on - strand, within nrdD at 4459.157 kb on + strand, within nrdD at 4459.158 kb on - strand, within nrdD at 4459.173 kb on + strand, within nrdD at 4459.202 kb on - strand, within nrdD at 4459.269 kb on + strand, within nrdD at 4459.269 kb on + strand, within nrdD at 4459.282 kb on + strand, within nrdD at 4459.282 kb on + strand, within nrdD at 4459.285 kb on + strand, within nrdD at 4459.285 kb on + strand, within nrdD at 4459.307 kb on + strand, within nrdD at 4459.307 kb on - strand, within nrdD at 4459.332 kb on + strand, within nrdD at 4459.332 kb on + strand, within nrdD at 4459.334 kb on + strand, within nrdD at 4459.352 kb on + strand, within nrdD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 4,456,966 - pmbA b4235 0.73 +0.2 4,457,011 - pmbA b4235 0.76 +0.1 4,457,029 - pmbA b4235 0.77 -0.1 4,457,050 + pmbA b4235 0.79 -0.4 4,457,077 + pmbA b4235 0.81 +0.4 4,457,079 - pmbA b4235 0.81 -0.4 4,457,090 + pmbA b4235 0.82 +0.6 4,457,171 - pmbA b4235 0.88 +0.2 4,457,171 - pmbA b4235 0.88 -0.7 4,457,279 + +0.4 4,457,279 + +0.2 4,457,279 + +0.3 4,457,281 + +0.5 4,457,287 - -1.0 4,457,287 - +0.6 4,457,287 - -1.2 4,457,294 + -0.5 4,457,312 - -0.3 4,457,433 - +0.4 4,457,438 + +0.3 4,457,479 + -0.2 4,457,487 - -0.2 4,457,487 - -0.9 4,457,530 - -0.5 4,457,539 - -0.2 4,457,572 + cybC b4236 0.16 -0.6 4,457,632 + cybC b4236 0.33 -0.5 4,457,632 + cybC b4236 0.33 -0.8 4,457,653 + cybC b4236 0.38 -0.1 4,457,711 + cybC b4236 0.54 -0.1 4,457,788 - cybC b4236 0.75 -0.4 4,457,788 - cybC b4236 0.75 -1.2 4,457,806 + cybC b4236 0.80 -0.2 4,457,806 + cybC b4236 0.80 +1.2 4,457,831 - cybC b4236 0.87 -0.2 4,457,831 - cybC b4236 0.87 -0.1 4,457,834 - cybC b4236 0.88 -0.2 4,457,853 + +0.1 4,457,853 + -1.2 4,457,853 + +0.2 4,457,903 - -0.4 4,457,944 - -0.8 4,457,944 - -0.0 4,458,063 + nrdG b4237 0.30 -1.3 4,458,074 + nrdG b4237 0.32 -1.8 4,458,095 + nrdG b4237 0.37 -1.7 4,458,095 + nrdG b4237 0.37 -1.3 4,458,126 - nrdG b4237 0.44 -2.0 4,458,126 - nrdG b4237 0.44 -2.3 4,458,154 + nrdG b4237 0.50 -1.6 4,458,179 + nrdG b4237 0.55 -1.2 4,458,179 + nrdG b4237 0.55 -0.7 4,458,191 + nrdG b4237 0.58 -1.7 4,458,191 + nrdG b4237 0.58 -1.3 4,458,229 - nrdG b4237 0.66 -1.6 4,458,233 + nrdG b4237 0.67 -1.6 4,458,241 - nrdG b4237 0.68 -1.0 4,458,241 - nrdG b4237 0.68 -1.9 4,458,241 - nrdG b4237 0.68 -1.8 4,458,247 - nrdG b4237 0.70 -1.4 4,458,247 - nrdG b4237 0.70 -2.0 4,458,264 + nrdG b4237 0.73 -1.4 4,458,294 - nrdG b4237 0.80 -0.4 4,458,294 - nrdG b4237 0.80 -0.6 4,458,301 + nrdG b4237 0.81 -0.9 4,458,354 - -2.0 4,458,365 + -0.9 4,458,365 + -1.4 4,458,373 - -0.3 4,458,373 - -1.1 4,458,373 - -2.8 4,458,373 - -2.1 4,458,373 - -0.4 4,458,373 - -0.4 4,458,432 + -0.4 4,458,479 + +0.1 4,458,479 + -2.3 4,458,489 - -0.2 4,458,494 - -0.3 4,458,571 - -0.9 4,458,571 - -1.2 4,458,591 + +0.5 4,458,613 + -0.8 4,458,630 - -1.6 4,458,630 - -2.7 4,458,677 + -0.1 4,458,677 + -1.2 4,458,677 - -0.7 4,458,698 - -0.9 4,458,709 + -2.8 4,458,763 - nrdD b4238 0.10 -0.7 4,458,768 + nrdD b4238 0.10 -1.6 4,458,779 + nrdD b4238 0.11 -1.6 4,458,935 - nrdD b4238 0.18 -0.3 4,458,971 - nrdD b4238 0.20 -1.2 4,458,994 - nrdD b4238 0.21 -1.0 4,458,994 - nrdD b4238 0.21 -1.1 4,459,004 - nrdD b4238 0.21 -1.9 4,459,011 + nrdD b4238 0.22 -0.8 4,459,019 - nrdD b4238 0.22 -1.9 4,459,019 - nrdD b4238 0.22 -0.9 4,459,053 + nrdD b4238 0.24 -1.7 4,459,053 + nrdD b4238 0.24 -1.0 4,459,063 - nrdD b4238 0.24 -1.2 4,459,063 - nrdD b4238 0.24 -0.7 4,459,066 - nrdD b4238 0.24 -0.7 4,459,091 + nrdD b4238 0.26 -0.9 4,459,130 - nrdD b4238 0.27 -2.7 4,459,157 + nrdD b4238 0.29 -1.3 4,459,158 - nrdD b4238 0.29 -1.7 4,459,173 + nrdD b4238 0.29 -1.3 4,459,202 - nrdD b4238 0.31 +0.9 4,459,269 + nrdD b4238 0.34 -1.0 4,459,269 + nrdD b4238 0.34 -1.5 4,459,282 + nrdD b4238 0.34 -0.2 4,459,282 + nrdD b4238 0.34 -1.6 4,459,285 + nrdD b4238 0.35 -1.9 4,459,285 + nrdD b4238 0.35 -1.5 4,459,307 + nrdD b4238 0.36 -0.9 4,459,307 - nrdD b4238 0.36 -1.3 4,459,332 + nrdD b4238 0.37 -2.8 4,459,332 + nrdD b4238 0.37 -2.0 4,459,334 + nrdD b4238 0.37 -0.9 4,459,352 + nrdD b4238 0.38 -2.0
Or see this region's nucleotide sequence