Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrA and ssb are separated by 253 nucleotides ssb and yjcB are separated by 98 nucleotides yjcB and yjcC are separated by 429 nucleotides
b4058: uvrA - excinuclease ABC subunit A (NCBI), at 4,269,072 to 4,271,894
uvrA
b4059: ssb - single-strand DNA-binding protein (NCBI), at 4,272,148 to 4,272,684
ssb
b4060: yjcB - orf, hypothetical protein (VIMSS), at 4,272,783 to 4,273,064
yjcB
b4061: yjcC - predicted signal transduction protein (EAL domain containing protein) (NCBI), at 4,273,494 to 4,275,080
yjcC
Position (kb)
4272
4273 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4271.260 kb on + strand, within uvrA at 4271.260 kb on + strand, within uvrA at 4271.270 kb on + strand, within uvrA at 4271.270 kb on + strand, within uvrA at 4271.270 kb on - strand, within uvrA at 4271.370 kb on + strand, within uvrA at 4271.371 kb on - strand, within uvrA at 4271.371 kb on - strand, within uvrA at 4271.376 kb on + strand, within uvrA at 4271.376 kb on + strand, within uvrA at 4271.378 kb on - strand, within uvrA at 4271.378 kb on - strand, within uvrA at 4271.409 kb on - strand, within uvrA at 4271.497 kb on - strand, within uvrA at 4271.497 kb on - strand, within uvrA at 4271.536 kb on - strand, within uvrA at 4271.596 kb on + strand, within uvrA at 4271.606 kb on - strand, within uvrA at 4271.615 kb on + strand at 4271.696 kb on - strand at 4271.737 kb on + strand at 4271.763 kb on - strand at 4271.764 kb on - strand at 4271.855 kb on + strand at 4271.936 kb on - strand at 4272.030 kb on - strand at 4272.030 kb on - strand at 4272.064 kb on + strand at 4272.105 kb on + strand at 4272.105 kb on + strand at 4272.786 kb on + strand at 4272.786 kb on + strand at 4272.840 kb on - strand, within yjcB at 4272.840 kb on - strand, within yjcB at 4272.847 kb on + strand, within yjcB at 4272.847 kb on + strand, within yjcB at 4272.850 kb on - strand, within yjcB at 4272.865 kb on - strand, within yjcB at 4272.865 kb on - strand, within yjcB at 4272.896 kb on - strand, within yjcB at 4272.906 kb on + strand, within yjcB at 4272.906 kb on + strand, within yjcB at 4272.956 kb on + strand, within yjcB at 4272.991 kb on - strand, within yjcB at 4273.022 kb on - strand, within yjcB at 4273.063 kb on - strand at 4273.092 kb on + strand at 4273.092 kb on + strand at 4273.092 kb on + strand at 4273.100 kb on - strand at 4273.127 kb on + strand at 4273.127 kb on + strand at 4273.133 kb on - strand at 4273.133 kb on - strand at 4273.280 kb on + strand at 4273.294 kb on - strand at 4273.294 kb on - strand at 4273.381 kb on + strand at 4273.381 kb on + strand at 4273.404 kb on + strand at 4273.436 kb on + strand at 4273.439 kb on - strand at 4273.439 kb on - strand at 4273.443 kb on + strand at 4273.451 kb on + strand at 4273.459 kb on - strand at 4273.459 kb on - strand at 4273.461 kb on + strand at 4273.462 kb on + strand at 4273.462 kb on + strand at 4273.466 kb on + strand at 4273.474 kb on - strand at 4273.474 kb on - strand at 4273.496 kb on + strand at 4273.496 kb on + strand at 4273.498 kb on + strand at 4273.498 kb on - strand at 4273.499 kb on - strand at 4273.501 kb on + strand at 4273.502 kb on + strand at 4273.502 kb on + strand at 4273.504 kb on + strand at 4273.511 kb on + strand at 4273.579 kb on - strand at 4273.597 kb on + strand at 4273.616 kb on + strand at 4273.624 kb on - strand at 4273.627 kb on + strand at 4273.627 kb on + strand at 4273.641 kb on + strand at 4273.672 kb on + strand, within yjcC at 4273.674 kb on + strand, within yjcC at 4273.674 kb on + strand, within yjcC at 4273.674 kb on + strand, within yjcC at 4273.674 kb on + strand, within yjcC at 4273.674 kb on + strand, within yjcC at 4273.674 kb on + strand, within yjcC at 4273.674 kb on + strand, within yjcC at 4273.674 kb on + strand, within yjcC at 4273.682 kb on - strand, within yjcC at 4273.682 kb on - strand, within yjcC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 4,271,260 + uvrA b4058 0.78 +0.1 4,271,260 + uvrA b4058 0.78 +0.5 4,271,270 + uvrA b4058 0.78 +0.8 4,271,270 + uvrA b4058 0.78 -0.0 4,271,270 - uvrA b4058 0.78 -1.9 4,271,370 + uvrA b4058 0.81 -0.4 4,271,371 - uvrA b4058 0.81 +1.2 4,271,371 - uvrA b4058 0.81 -1.6 4,271,376 + uvrA b4058 0.82 +0.8 4,271,376 + uvrA b4058 0.82 +0.1 4,271,378 - uvrA b4058 0.82 -0.3 4,271,378 - uvrA b4058 0.82 -0.2 4,271,409 - uvrA b4058 0.83 -0.4 4,271,497 - uvrA b4058 0.86 -1.5 4,271,497 - uvrA b4058 0.86 -2.0 4,271,536 - uvrA b4058 0.87 +0.0 4,271,596 + uvrA b4058 0.89 +0.7 4,271,606 - uvrA b4058 0.90 -0.8 4,271,615 + +0.2 4,271,696 - -0.6 4,271,737 + +0.9 4,271,763 - -0.8 4,271,764 - +0.2 4,271,855 + +1.3 4,271,936 - +0.5 4,272,030 - -1.1 4,272,030 - -0.1 4,272,064 + -0.8 4,272,105 + -0.6 4,272,105 + -0.0 4,272,786 + -0.1 4,272,786 + -0.2 4,272,840 - yjcB b4060 0.20 -0.4 4,272,840 - yjcB b4060 0.20 -1.8 4,272,847 + yjcB b4060 0.23 -1.0 4,272,847 + yjcB b4060 0.23 -0.2 4,272,850 - yjcB b4060 0.24 -0.0 4,272,865 - yjcB b4060 0.29 -0.2 4,272,865 - yjcB b4060 0.29 +1.2 4,272,896 - yjcB b4060 0.40 -1.2 4,272,906 + yjcB b4060 0.44 +1.0 4,272,906 + yjcB b4060 0.44 -0.1 4,272,956 + yjcB b4060 0.61 -0.3 4,272,991 - yjcB b4060 0.74 -0.2 4,273,022 - yjcB b4060 0.85 +0.9 4,273,063 - -0.3 4,273,092 + -1.0 4,273,092 + +0.5 4,273,092 + +1.3 4,273,100 - -0.4 4,273,127 + +0.3 4,273,127 + -0.0 4,273,133 - +0.3 4,273,133 - -0.6 4,273,280 + +0.1 4,273,294 - +0.0 4,273,294 - -0.9 4,273,381 + -0.2 4,273,381 + +0.5 4,273,404 + -0.6 4,273,436 + -0.7 4,273,439 - -2.1 4,273,439 - +0.5 4,273,443 + +0.3 4,273,451 + -0.7 4,273,459 - -1.2 4,273,459 - +0.8 4,273,461 + -1.1 4,273,462 + +0.4 4,273,462 + +0.2 4,273,466 + +0.4 4,273,474 - +1.4 4,273,474 - -0.8 4,273,496 + -1.2 4,273,496 + -2.1 4,273,498 + -1.4 4,273,498 - +0.4 4,273,499 - -1.4 4,273,501 + +0.1 4,273,502 + +2.2 4,273,502 + -0.1 4,273,504 + -2.1 4,273,511 + -0.6 4,273,579 - +0.8 4,273,597 + +0.3 4,273,616 + +0.1 4,273,624 - +1.8 4,273,627 + -1.9 4,273,627 + +0.6 4,273,641 + +0.2 4,273,672 + yjcC b4061 0.11 +0.2 4,273,674 + yjcC b4061 0.11 -1.7 4,273,674 + yjcC b4061 0.11 +1.4 4,273,674 + yjcC b4061 0.11 -0.1 4,273,674 + yjcC b4061 0.11 +1.2 4,273,674 + yjcC b4061 0.11 +1.0 4,273,674 + yjcC b4061 0.11 +0.5 4,273,674 + yjcC b4061 0.11 -1.8 4,273,674 + yjcC b4061 0.11 -1.4 4,273,682 - yjcC b4061 0.12 +1.8 4,273,682 - yjcC b4061 0.12 +1.8
Or see this region's nucleotide sequence