Strain Fitness in Escherichia coli BW25113 around b3621

Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Nalidixic 0.0000078125 mM
3,793,011	+				-1.4
3,793,021	+				+0.3
3,793,021	+				-0.1
3,793,021	+				-0.4
3,793,034	+				-1.1
3,793,124	-	rfaF	b3620	0.16	-0.4
3,793,169	+	rfaF	b3620	0.21	-2.2
3,793,234	-	rfaF	b3620	0.27	-0.9
3,793,259	-	rfaF	b3620	0.29	-2.1
3,793,305	-	rfaF	b3620	0.34	-1.5
3,793,307	+	rfaF	b3620	0.34	-0.1
3,793,307	+	rfaF	b3620	0.34	-0.1
3,793,307	+	rfaF	b3620	0.34	-1.2
3,793,307	-	rfaF	b3620	0.34	-1.4
3,793,311	+	rfaF	b3620	0.34	-1.1
3,793,328	-	rfaF	b3620	0.36	+0.9
3,793,350	+				+0.9
3,793,353	+	rfaF	b3620	0.38	+0.6
3,793,358	-	rfaF	b3620	0.39	-0.7
3,793,358	-	rfaF	b3620	0.39	-1.2
3,793,393	+	rfaF	b3620	0.42	+0.9
3,793,393	+	rfaF	b3620	0.42	-0.1
3,793,393	+	rfaF	b3620	0.42	-1.1
3,793,513	+	rfaF	b3620	0.54	-1.0
3,793,543	+	rfaF	b3620	0.56	-2.5
3,793,546	+	rfaF	b3620	0.57	-2.3
3,793,554	-	rfaF	b3620	0.57	-1.7
3,793,554	-	rfaF	b3620	0.57	-0.1
3,793,589	+	rfaF	b3620	0.61	-0.4
3,793,589	+	rfaF	b3620	0.61	-1.2
3,793,595	-	rfaF	b3620	0.61	+0.9
3,793,600	+	rfaF	b3620	0.62	-1.4
3,793,663	+	rfaF	b3620	0.68	-1.1
3,793,725	-	rfaF	b3620	0.74	-0.1
3,793,725	-	rfaF	b3620	0.74	-0.1
3,793,734	-	rfaF	b3620	0.75	-0.1
3,793,734	-	rfaF	b3620	0.75	-1.1
3,793,765	-	rfaF	b3620	0.78	-1.3
3,793,775	+	rfaF	b3620	0.79	-1.1
3,793,775	+	rfaF	b3620	0.79	-1.7
3,793,813	+	rfaF	b3620	0.82	+0.9
3,793,813	+				+0.3
3,793,821	-	rfaF	b3620	0.83	-0.1
3,793,857	-	rfaF	b3620	0.86	-1.9
3,793,857	-	rfaF	b3620	0.86	-0.9
3,793,916	+				-1.4
3,793,921	-				-0.1
3,793,921	-				-0.7
3,793,926	-				-1.1
3,793,984	-				-0.6
3,794,047	-				+0.3
3,794,050	+				-1.7
3,794,050	+				-0.9
3,794,054	+				+0.3
3,794,054	+				-0.9
3,794,107	+	rfaC	b3621	0.11	-2.0
3,794,120	+	rfaC	b3621	0.12	-1.7
3,794,120	+	rfaC	b3621	0.12	-1.4
3,794,128	-	rfaC	b3621	0.13	-0.4
3,794,279	+	rfaC	b3621	0.29	-2.2
3,794,287	-	rfaC	b3621	0.30	-0.1
3,794,322	-	rfaC	b3621	0.33	+0.3
3,794,322	-	rfaC	b3621	0.33	+0.3
3,794,335	-	rfaC	b3621	0.35	+0.3
3,794,335	-	rfaC	b3621	0.35	-0.4
3,794,346	-	rfaC	b3621	0.36	-0.1
3,794,365	+	rfaC	b3621	0.38	-1.5
3,794,365	+	rfaC	b3621	0.38	-0.9
3,794,402	+	rfaC	b3621	0.42	-0.5
3,794,402	+	rfaC	b3621	0.42	-0.4
3,794,428	-	rfaC	b3621	0.44	-0.9
3,794,493	-	rfaC	b3621	0.51	-1.1
3,794,519	+	rfaC	b3621	0.54	+0.3
3,794,520	+	rfaC	b3621	0.54	+0.1
3,794,570	+	rfaC	b3621	0.59	-0.1
3,794,597	+				+1.9
3,794,597	+	rfaC	b3621	0.62	-0.1
3,794,612	+	rfaC	b3621	0.64	+0.3
3,794,713	-	rfaC	b3621	0.74	+1.9
3,794,713	-	rfaC	b3621	0.74	+0.9
3,794,728	+	rfaC	b3621	0.76	-0.7
3,794,728	+	rfaC	b3621	0.76	-0.7
3,794,729	+	rfaC	b3621	0.76	-1.1
3,794,729	+	rfaC	b3621	0.76	-0.4
3,794,730	+	rfaC	b3621	0.76	-1.2
3,794,765	+	rfaC	b3621	0.79	-0.9
3,794,765	+	rfaC	b3621	0.79	-1.1
3,794,765	+	rfaC	b3621	0.79	-2.2
3,794,765	+	rfaC	b3621	0.79	-0.9
3,794,773	+	rfaC	b3621	0.80	+0.3
3,794,773	+	rfaC	b3621	0.80	-0.1
3,794,778	+	rfaC	b3621	0.81	-1.1
3,794,781	+	rfaC	b3621	0.81	-1.1
3,794,809	+	rfaC	b3621	0.84	-0.7
3,794,809	+	rfaC	b3621	0.84	-0.1
3,794,809	+	rfaC	b3621	0.84	-1.1
3,794,814	+	rfaC	b3621	0.85	-1.9
3,794,814	+	rfaC	b3621	0.85	+0.9
3,794,814	+	rfaC	b3621	0.85	+0.3
3,794,814	+	rfaC	b3621	0.85	-0.4
3,794,820	+	rfaC	b3621	0.85	-0.4
3,794,820	+	rfaC	b3621	0.85	-2.0
3,794,860	+	rfaC	b3621	0.89	-0.7
3,794,860	+	rfaC	b3621	0.89	-1.2
3,794,867	+				+0.3
3,794,867	+				+0.9
3,794,867	+				-0.1
3,794,867	+				-0.4
3,794,867	+				-0.4
3,794,868	+				-0.7
3,794,868	+				-0.7
3,794,868	+				-1.7
3,794,868	+				-0.1
3,794,877	+				-0.4
3,794,879	+				+1.9
3,794,879	+				-0.1
3,794,879	+				+0.3
3,794,886	+				+0.3
3,794,886	+				+0.9
3,794,886	+				-0.7
3,794,887	+				-0.9
3,794,887	+				+0.9
3,794,887	+				-0.5
3,794,887	+				-1.2
3,794,887	+				-1.8
3,794,887	+				+1.2
3,794,887	+				-2.5
3,794,887	+				+0.3
3,794,887	+				-0.9
3,794,887	+				+0.1
3,794,887	+				-0.4
3,794,887	+				-2.1
3,794,887	+				-0.5
3,794,889	+				-1.7
3,794,889	+				-0.4
3,794,889	+				+0.3
3,794,889	+				+0.9
3,794,891	+				-1.4
3,794,917	+				-0.1
3,794,917	+				+0.3
3,794,917	+				-2.5
3,794,919	+				-1.0
3,794,927	+				-2.4
3,794,938	+				-0.3
3,794,953	+				+0.5
3,795,003	+				-0.8
3,795,003	+				-0.1
3,795,003	+				-1.1
3,795,005	+				+0.1
3,795,146	-	rfaL	b3622	0.14	+1.9
3,795,275	-	rfaL	b3622	0.24	+1.9
3,795,423	+	rfaL	b3622	0.36	+1.9
3,795,423	+	rfaL	b3622	0.36	+1.9
3,795,463	+	rfaL	b3622	0.39	+1.9
3,795,474	+	rfaL	b3622	0.40	+1.9
3,795,489	+	rfaL	b3622	0.41	+1.9
3,795,489	+	rfaL	b3622	0.41	+1.9
3,795,489	+	rfaL	b3622	0.41	+1.9
3,795,494	+	rfaL	b3622	0.42	+1.9
3,795,494	+	rfaL	b3622	0.42	+1.9
3,795,514	+	rfaL	b3622	0.43	+1.9
3,795,521	-	rfaL	b3622	0.44	+1.9
3,795,522	-	rfaL	b3622	0.44	+1.9
3,795,597	+	rfaL	b3622	0.50	+1.9
3,795,609	+	rfaL	b3622	0.51	+1.9
3,795,609	+	rfaL	b3622	0.51	+1.9
3,795,627	-	rfaL	b3622	0.52	+1.9
3,795,628	-	rfaL	b3622	0.52	+1.9
3,795,706	-	rfaL	b3622	0.58	+1.9
3,795,747	-	rfaL	b3622	0.62	+1.9
3,795,773	-	rfaL	b3622	0.64	+1.9
3,795,792	-	rfaL	b3622	0.65	+1.9
3,795,792	-	rfaL	b3622	0.65	+1.9
3,795,794	+	rfaL	b3622	0.65	+1.9
3,795,801	-	rfaL	b3622	0.66	+1.9
3,795,801	-	rfaL	b3622	0.66	+1.9
3,795,818	+	rfaL	b3622	0.67	+1.9
3,795,835	-	rfaL	b3622	0.69	+1.9
3,795,835	-	rfaL	b3622	0.69	+1.9
3,795,835	-	rfaL	b3622	0.69	+1.9
3,795,835	-	rfaL	b3622	0.69	+1.9
3,795,841	-	rfaL	b3622	0.69	+1.9
3,795,893	+	rfaL	b3622	0.73	+1.9
3,795,901	-	rfaL	b3622	0.74	+1.9
3,795,901	-	rfaL	b3622	0.74	+1.9
3,795,901	-	rfaL	b3622	0.74	+1.9
3,795,936	+	rfaL	b3622	0.77	+0.9
3,795,938	+	rfaL	b3622	0.77	+1.9
3,795,939	+	rfaL	b3622	0.77	+1.9
3,795,939	+	rfaL	b3622	0.77	+1.9
3,795,939	+	rfaL	b3622	0.77	+0.3
3,795,939	+	rfaL	b3622	0.77	+1.9
3,795,939	+	rfaL	b3622	0.77	+1.9
3,795,942	-	rfaL	b3622	0.77	+1.9
3,795,943	+	rfaL	b3622	0.77	+1.9
3,795,944	+	rfaL	b3622	0.77	+0.9
3,795,944	+	rfaL	b3622	0.77	+1.9
3,795,944	+	rfaL	b3622	0.77	+1.9
3,795,944	+	rfaL	b3622	0.77	+1.9
3,795,944	+	rfaL	b3622	0.77	+1.9
3,795,945	+	rfaL	b3622	0.77	+0.9
3,795,945	+	rfaL	b3622	0.77	+1.5
3,795,950	+	rfaL	b3622	0.78	-1.5
3,795,950	+	rfaL	b3622	0.78	+1.9
3,795,955	+	rfaL	b3622	0.78	+1.9
3,795,955	+	rfaL	b3622	0.78	+0.9