Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdaB and ygdG are separated by 111 nucleotides ygdG and fucO are separated by 54 nucleotides
b2797: sdaB - L-serine deaminase II (NCBI), at 2,927,598 to 2,928,965
sdaB
b2798: ygdG - Ssb-binding protein, misidentified as ExoIX (RefSeq), at 2,929,077 to 2,929,832
ygdG
b2799: fucO - L-1,2-propanediol oxidoreductase (NCBI), at 2,929,887 to 2,931,035
fucO
Position (kb)
2929
2930 Strain fitness (log2 ratio)
-2
-1
0
1 at 2928.085 kb on - strand, within sdaB at 2928.085 kb on - strand, within sdaB at 2928.147 kb on - strand, within sdaB at 2928.147 kb on - strand, within sdaB at 2928.210 kb on + strand, within sdaB at 2928.261 kb on - strand, within sdaB at 2928.261 kb on - strand, within sdaB at 2928.312 kb on - strand, within sdaB at 2928.313 kb on + strand, within sdaB at 2928.313 kb on + strand, within sdaB at 2928.313 kb on + strand, within sdaB at 2928.332 kb on - strand, within sdaB at 2928.410 kb on + strand, within sdaB at 2928.435 kb on + strand, within sdaB at 2928.445 kb on - strand, within sdaB at 2928.516 kb on + strand, within sdaB at 2928.554 kb on - strand, within sdaB at 2928.554 kb on - strand, within sdaB at 2928.570 kb on + strand, within sdaB at 2928.724 kb on + strand, within sdaB at 2928.725 kb on - strand, within sdaB at 2928.725 kb on - strand, within sdaB at 2928.729 kb on + strand, within sdaB at 2928.729 kb on + strand, within sdaB at 2928.729 kb on + strand, within sdaB at 2928.729 kb on + strand, within sdaB at 2928.744 kb on - strand, within sdaB at 2928.764 kb on + strand, within sdaB at 2928.767 kb on + strand, within sdaB at 2928.775 kb on - strand, within sdaB at 2928.787 kb on - strand, within sdaB at 2928.814 kb on + strand, within sdaB at 2928.825 kb on + strand, within sdaB at 2928.825 kb on + strand, within sdaB at 2928.835 kb on + strand at 2928.887 kb on - strand at 2928.887 kb on - strand at 2928.919 kb on - strand at 2928.919 kb on - strand at 2928.956 kb on - strand at 2928.956 kb on - strand at 2929.025 kb on - strand at 2929.158 kb on - strand, within ygdG at 2929.189 kb on - strand, within ygdG at 2929.335 kb on + strand, within ygdG at 2929.353 kb on + strand, within ygdG at 2929.391 kb on - strand, within ygdG at 2929.652 kb on + strand, within ygdG at 2929.680 kb on - strand, within ygdG at 2929.683 kb on + strand, within ygdG at 2929.683 kb on + strand, within ygdG at 2929.891 kb on - strand at 2929.915 kb on - strand at 2929.970 kb on - strand at 2929.972 kb on + strand at 2929.974 kb on - strand at 2929.980 kb on - strand at 2929.980 kb on - strand at 2929.996 kb on + strand at 2929.996 kb on + strand at 2930.006 kb on + strand, within fucO at 2930.006 kb on + strand, within fucO at 2930.137 kb on + strand, within fucO at 2930.161 kb on + strand, within fucO at 2930.161 kb on + strand, within fucO at 2930.189 kb on - strand, within fucO at 2930.198 kb on + strand, within fucO at 2930.215 kb on - strand, within fucO at 2930.248 kb on - strand, within fucO at 2930.280 kb on + strand, within fucO at 2930.288 kb on - strand, within fucO at 2930.298 kb on - strand, within fucO at 2930.298 kb on - strand, within fucO at 2930.362 kb on + strand, within fucO at 2930.367 kb on + strand, within fucO at 2930.406 kb on - strand, within fucO at 2930.406 kb on - strand, within fucO at 2930.417 kb on + strand at 2930.427 kb on + strand, within fucO at 2930.427 kb on + strand, within fucO at 2930.622 kb on - strand, within fucO at 2930.660 kb on + strand, within fucO at 2930.676 kb on + strand, within fucO at 2930.677 kb on - strand, within fucO at 2930.806 kb on - strand, within fucO at 2930.806 kb on - strand, within fucO at 2930.832 kb on - strand, within fucO
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 2,928,085 - sdaB b2797 0.36 +0.2 2,928,085 - sdaB b2797 0.36 -0.9 2,928,147 - sdaB b2797 0.40 -0.9 2,928,147 - sdaB b2797 0.40 -0.0 2,928,210 + sdaB b2797 0.45 +0.1 2,928,261 - sdaB b2797 0.48 -1.6 2,928,261 - sdaB b2797 0.48 +0.0 2,928,312 - sdaB b2797 0.52 -0.1 2,928,313 + sdaB b2797 0.52 -0.2 2,928,313 + sdaB b2797 0.52 -1.5 2,928,313 + sdaB b2797 0.52 +0.4 2,928,332 - sdaB b2797 0.54 -0.4 2,928,410 + sdaB b2797 0.59 -0.4 2,928,435 + sdaB b2797 0.61 -0.9 2,928,445 - sdaB b2797 0.62 +0.2 2,928,516 + sdaB b2797 0.67 -0.7 2,928,554 - sdaB b2797 0.70 -0.9 2,928,554 - sdaB b2797 0.70 -0.6 2,928,570 + sdaB b2797 0.71 -0.6 2,928,724 + sdaB b2797 0.82 -1.4 2,928,725 - sdaB b2797 0.82 -0.9 2,928,725 - sdaB b2797 0.82 +0.5 2,928,729 + sdaB b2797 0.83 -0.5 2,928,729 + sdaB b2797 0.83 +0.0 2,928,729 + sdaB b2797 0.83 -0.2 2,928,729 + sdaB b2797 0.83 -0.1 2,928,744 - sdaB b2797 0.84 -0.2 2,928,764 + sdaB b2797 0.85 +0.1 2,928,767 + sdaB b2797 0.85 -0.4 2,928,775 - sdaB b2797 0.86 +0.2 2,928,787 - sdaB b2797 0.87 -0.6 2,928,814 + sdaB b2797 0.89 +1.7 2,928,825 + sdaB b2797 0.90 +0.6 2,928,825 + sdaB b2797 0.90 +0.5 2,928,835 + -0.1 2,928,887 - +0.2 2,928,887 - +0.5 2,928,919 - -2.0 2,928,919 - +0.6 2,928,956 - +0.7 2,928,956 - -0.5 2,929,025 - +0.5 2,929,158 - ygdG b2798 0.11 -0.3 2,929,189 - ygdG b2798 0.15 +0.1 2,929,335 + ygdG b2798 0.34 +0.0 2,929,353 + ygdG b2798 0.37 -0.0 2,929,391 - ygdG b2798 0.42 +0.1 2,929,652 + ygdG b2798 0.76 +0.4 2,929,680 - ygdG b2798 0.80 +0.9 2,929,683 + ygdG b2798 0.80 +0.4 2,929,683 + ygdG b2798 0.80 +0.2 2,929,891 - -0.0 2,929,915 - -0.5 2,929,970 - +0.3 2,929,972 + +0.8 2,929,974 - -1.1 2,929,980 - +0.8 2,929,980 - +0.6 2,929,996 + -0.2 2,929,996 + +1.7 2,930,006 + fucO b2799 0.10 +0.9 2,930,006 + fucO b2799 0.10 -0.9 2,930,137 + fucO b2799 0.22 -0.1 2,930,161 + fucO b2799 0.24 -0.3 2,930,161 + fucO b2799 0.24 -0.0 2,930,189 - fucO b2799 0.26 -0.5 2,930,198 + fucO b2799 0.27 +0.3 2,930,215 - fucO b2799 0.29 -1.4 2,930,248 - fucO b2799 0.31 -0.0 2,930,280 + fucO b2799 0.34 -0.4 2,930,288 - fucO b2799 0.35 -1.0 2,930,298 - fucO b2799 0.36 +0.6 2,930,298 - fucO b2799 0.36 +0.8 2,930,362 + fucO b2799 0.41 +0.2 2,930,367 + fucO b2799 0.42 +1.4 2,930,406 - fucO b2799 0.45 -1.1 2,930,406 - fucO b2799 0.45 -0.2 2,930,417 + -0.3 2,930,427 + fucO b2799 0.47 +0.2 2,930,427 + fucO b2799 0.47 +0.1 2,930,622 - fucO b2799 0.64 +1.7 2,930,660 + fucO b2799 0.67 +0.1 2,930,676 + fucO b2799 0.69 -0.9 2,930,677 - fucO b2799 0.69 +0.0 2,930,806 - fucO b2799 0.80 +1.3 2,930,806 - fucO b2799 0.80 +1.7 2,930,832 - fucO b2799 0.82 +0.6
Or see this region's nucleotide sequence