Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt alaS and recX are separated by 127 nucleotides recX and recA are separated by 68 nucleotides recA and ygaD are separated by 79 nucleotides ygaD and mltB are separated by 144 nucleotides
b2697: alaS - alanyl-tRNA synthetase (NCBI), at 2,817,403 to 2,820,033
alaS
b2698: recX - RecA regulator RecX (NCBI), at 2,820,161 to 2,820,661
recX
b2699: recA - recombinase A (NCBI), at 2,820,730 to 2,821,791
recA
b2700: ygaD - competence damage-inducible protein A (NCBI), at 2,821,871 to 2,822,368
ygaD
b2701: mltB - membrane-bound lytic murein transglycosylase B (NCBI), at 2,822,513 to 2,823,598
mltB
Position (kb)
2820
2821
2822 Strain fitness (log2 ratio)
-1
0
1
2 at 2820.070 kb on - strand at 2820.143 kb on + strand at 2820.258 kb on + strand, within recX at 2820.258 kb on + strand, within recX at 2820.457 kb on - strand, within recX at 2820.634 kb on - strand at 2820.929 kb on + strand, within recA at 2821.141 kb on - strand, within recA at 2821.166 kb on - strand, within recA at 2821.522 kb on + strand, within recA at 2821.638 kb on + strand, within recA at 2821.701 kb on - strand at 2821.810 kb on - strand at 2821.810 kb on - strand at 2822.013 kb on + strand, within ygaD at 2822.231 kb on - strand, within ygaD at 2822.232 kb on + strand, within ygaD at 2822.333 kb on - strand at 2822.358 kb on + strand at 2822.437 kb on - strand at 2822.488 kb on + strand at 2822.520 kb on + strand at 2822.520 kb on + strand at 2822.545 kb on - strand at 2822.598 kb on - strand at 2822.641 kb on - strand, within mltB at 2822.668 kb on + strand, within mltB at 2822.764 kb on - strand, within mltB at 2822.765 kb on - strand, within mltB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 2,820,070 - +1.7 2,820,143 + +1.7 2,820,258 + recX b2698 0.19 -1.5 2,820,258 + recX b2698 0.19 +1.8 2,820,457 - recX b2698 0.59 -0.2 2,820,634 - +0.3 2,820,929 + recA b2699 0.19 +0.7 2,821,141 - recA b2699 0.39 -0.3 2,821,166 - recA b2699 0.41 +0.1 2,821,522 + recA b2699 0.75 +0.7 2,821,638 + recA b2699 0.85 +1.7 2,821,701 - +0.7 2,821,810 - +0.1 2,821,810 - -0.6 2,822,013 + ygaD b2700 0.29 -0.3 2,822,231 - ygaD b2700 0.72 +0.2 2,822,232 + ygaD b2700 0.72 +0.6 2,822,333 - -0.4 2,822,358 + -0.2 2,822,437 - +0.4 2,822,488 + -0.3 2,822,520 + -0.8 2,822,520 + +0.7 2,822,545 - +0.4 2,822,598 - -0.4 2,822,641 - mltB b2701 0.12 -0.1 2,822,668 + mltB b2701 0.14 +0.1 2,822,764 - mltB b2701 0.23 +0.7 2,822,765 - mltB b2701 0.23 -1.0
Or see this region's nucleotide sequence