Strain Fitness in Escherichia coli BW25113 around b2580

Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Nalidixic 0.0000078125 mM
2,713,815	+	yfiK	b2578	0.63	-0.0
2,713,815	+	yfiK	b2578	0.63	+0.4
2,713,815	+	yfiK	b2578	0.63	+1.0
2,713,815	+	yfiK	b2578	0.63	+1.1
2,713,833	+	yfiK	b2578	0.66	+0.6
2,713,833	+	yfiK	b2578	0.66	+0.4
2,714,065	-				-0.4
2,714,072	+				+0.8
2,714,072	+				-1.7
2,714,093	+				-0.9
2,714,105	+				-0.4
2,714,129	+	yfiD	b2579	0.11	+0.2
2,714,220	+	yfiD	b2579	0.34	-0.4
2,714,220	+	yfiD	b2579	0.34	+0.1
2,714,255	+	yfiD	b2579	0.43	+0.2
2,714,430	+	yfiD	b2579	0.89	-1.2
2,714,557	-				+0.4
2,714,557	-				+1.2
2,714,652	-				-0.1
2,714,777	-				-0.6
2,714,937	-	ung	b2580	0.23	-0.5
2,714,940	+	ung	b2580	0.24	-0.5
2,714,978	+	ung	b2580	0.29	+0.4
2,715,050	+	ung	b2580	0.40	+0.0
2,715,089	+	ung	b2580	0.45	-0.2
2,715,121	+	ung	b2580	0.50	+0.5
2,715,161	+	ung	b2580	0.56	+0.2
2,715,195	-	ung	b2580	0.61	+1.1
2,715,311	+	ung	b2580	0.78	+0.1
2,715,451	+				+1.1
2,715,608	+				+0.0
2,715,654	+	yfiF	b2581	0.14	+0.5
2,715,706	+	yfiF	b2581	0.19	+0.2
2,715,746	-	yfiF	b2581	0.22	-1.6
2,715,854	+	yfiF	b2581	0.33	-0.0
2,715,879	-	yfiF	b2581	0.35	-0.6
2,715,997	+	yfiF	b2581	0.47	-0.1
2,715,997	+	yfiF	b2581	0.47	-0.4
2,715,997	+	yfiF	b2581	0.47	+0.4
2,715,997	+	yfiF	b2581	0.47	+0.9
2,716,005	-	yfiF	b2581	0.47	+0.1
2,716,005	-	yfiF	b2581	0.47	+0.6
2,716,005	-	yfiF	b2581	0.47	-0.8
2,716,005	-	yfiF	b2581	0.47	+0.3
2,716,141	+	yfiF	b2581	0.61	+0.6
2,716,141	+				-1.4
2,716,183	-	yfiF	b2581	0.65	-0.5
2,716,183	-	yfiF	b2581	0.65	-0.3
2,716,229	+	yfiF	b2581	0.69	-0.3
2,716,265	-	yfiF	b2581	0.72	+0.6
2,716,265	-	yfiF	b2581	0.72	+0.0
2,716,313	+	yfiF	b2581	0.77	-0.0
2,716,313	+	yfiF	b2581	0.77	+1.1
2,716,387	-	yfiF	b2581	0.84	+1.1
2,716,387	-	yfiF	b2581	0.84	-0.5
2,716,440	+	yfiF	b2581	0.89	-1.2
2,716,446	+	yfiF	b2581	0.90	-0.1