Strain Fitness in Escherichia coli BW25113 around b2354

Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Nalidixic 0.0000078125 mM
2,468,407	+				+1.6
2,468,525	-				-0.2
2,468,525	-				-2.5
2,468,527	-				-0.0
2,468,527	-				-0.4
2,468,527	-				-0.7
2,468,553	-				+0.2
2,468,553	-				-0.1
2,468,557	-				-0.9
2,468,566	-				-0.1
2,468,566	-				+0.0
2,468,592	-				+0.0
2,468,592	-				+0.7
2,468,593	-				+1.2
2,468,595	-				-0.2
2,468,595	-				-0.4
2,468,651	-				-1.7
2,468,651	-				-1.0
2,468,658	-				-0.6
2,468,660	+				+1.6
2,468,662	-				-0.3
2,468,665	+				+1.6
2,468,673	-				-0.7
2,468,676	+				-0.4
2,468,676	+				-1.0
2,468,676	+				-1.4
2,468,711	+				+0.1
2,468,715	-				+0.1
2,468,717	+				-0.4
2,468,719	-				+0.1
2,468,722	+				-1.2
2,468,737	+				-0.3
2,468,757	-				-0.3
2,468,757	-				-0.8
2,468,764	-				-0.8
2,468,778	-				-0.1
2,468,778	-				-1.4
2,468,804	+				+0.6
2,468,808	-				+0.7
2,468,808	-				-1.0
2,468,808	-				+1.2
2,468,812	+				-0.7
2,468,818	+				+0.3
2,468,818	+				-0.2
2,468,819	+				-0.6
2,468,819	+				-0.1
2,468,821	-				+0.2
2,468,829	-				-0.6
2,468,838	-				+0.1
2,468,887	+	tfaS	b2353	0.17	+1.4
2,468,890	+	tfaS	b2353	0.18	-0.7
2,468,895	+	tfaS	b2353	0.20	-0.4
2,468,895	+	tfaS	b2353	0.20	+0.7
2,468,895	-	tfaS	b2353	0.20	-0.4
2,468,897	-	tfaS	b2353	0.21	-1.0
2,468,897	-	tfaS	b2353	0.21	-0.8
2,468,903	-	tfaS	b2353	0.23	-0.5
2,468,903	-	tfaS	b2353	0.23	-1.3
2,468,905	+	tfaS	b2353	0.23	+0.7
2,468,905	+	tfaS	b2353	0.23	-0.2
2,468,905	+	tfaS	b2353	0.23	+0.2
2,468,906	-	tfaS	b2353	0.24	+0.4
2,468,912	+	tfaS	b2353	0.26	+1.2
2,468,913	-	tfaS	b2353	0.26	-0.4
2,468,913	-	tfaS	b2353	0.26	+0.6
2,468,918	+	tfaS	b2353	0.28	-0.7
2,468,926	-	tfaS	b2353	0.31	-0.2
2,468,936	+	tfaS	b2353	0.34	+0.7
2,468,936	+	tfaS	b2353	0.34	+0.2
2,468,936	-	tfaS	b2353	0.34	-0.3
2,468,939	+	tfaS	b2353	0.35	-0.1
2,468,940	+	tfaS	b2353	0.35	-1.2
2,468,940	+	tfaS	b2353	0.35	+0.6
2,468,946	-	tfaS	b2353	0.37	-0.7
2,468,958	-	tfaS	b2353	0.42	-0.5
2,468,960	-	tfaS	b2353	0.42	+0.3
2,469,002	+	tfaS	b2353	0.57	-0.2
2,469,006	-	tfaS	b2353	0.58	-0.1
2,469,015	+	tfaS	b2353	0.61	+0.5
2,469,019	+	tfaS	b2353	0.63	+0.2
2,469,042	-	tfaS	b2353	0.70	-0.6
2,469,042	-	tfaS	b2353	0.70	-0.6
2,469,057	-	tfaS	b2353	0.76	-2.8
2,469,057	-	tfaS	b2353	0.76	+0.1
2,469,095	+	tfaS	b2353	0.89	+1.2
2,469,095	+	tfaS	b2353	0.89	+0.0
2,469,100	+				+0.6
2,469,105	+				-1.8
2,469,115	+				-0.5
2,469,131	+				-0.6
2,469,164	+	yfdK	b2354	0.15	+1.6
2,469,164	+	yfdK	b2354	0.15	+0.6
2,469,164	+	yfdK	b2354	0.15	+0.6
2,469,164	+	yfdK	b2354	0.15	+1.6
2,469,164	+	yfdK	b2354	0.15	-0.0
2,469,164	+	yfdK	b2354	0.15	-0.4
2,469,164	+	yfdK	b2354	0.15	-0.0
2,469,164	+	yfdK	b2354	0.15	+0.6
2,469,170	-	yfdK	b2354	0.16	-0.8
2,469,170	-	yfdK	b2354	0.16	-0.9
2,469,172	-	yfdK	b2354	0.17	-0.8
2,469,179	+	yfdK	b2354	0.18	+0.6
2,469,210	+	yfdK	b2354	0.25	+1.6
2,469,210	+	yfdK	b2354	0.25	+1.6
2,469,236	-	yfdK	b2354	0.31	+0.1
2,469,241	-	yfdK	b2354	0.32	-1.5
2,469,331	-	yfdK	b2354	0.53	-1.8
2,469,331	-	yfdK	b2354	0.53	+0.6
2,469,331	-	yfdK	b2354	0.53	+0.4
2,469,332	-	yfdK	b2354	0.53	-0.1
2,469,332	-	yfdK	b2354	0.53	-2.1
2,469,332	-	yfdK	b2354	0.53	+1.8
2,469,332	-	yfdK	b2354	0.53	+1.6
2,469,354	-	yfdK	b2354	0.58	+2.6
2,469,442	-	yfdK	b2354	0.78	-0.9
2,469,442	-	yfdK	b2354	0.78	+1.3
2,469,451	-	yfdK	b2354	0.80	-1.3
2,469,457	+	yfdK	b2354	0.81	-0.0
2,469,457	+	yfdK	b2354	0.81	+0.6
2,469,459	+	yfdK	b2354	0.82	+0.6
2,469,460	-	yfdK	b2354	0.82	+1.9
2,469,461	-	yfdK	b2354	0.82	-0.4
2,469,461	-	yfdK	b2354	0.82	-0.0
2,469,463	+	yfdK	b2354	0.83	+1.6
2,469,596	+				+1.6
2,469,596	+				+1.6
2,469,610	-				-0.1
2,469,626	-	yfdL	b2355	0.10	+1.0
2,469,659	-	yfdL	b2355	0.16	+0.3
2,469,663	-	yfdL	b2355	0.17	-1.5
2,469,663	-	yfdL	b2355	0.17	-0.4
2,469,685	+	yfdL	b2355	0.21	+1.6
2,469,688	-	yfdL	b2355	0.21	+1.0
2,469,695	-	yfdL	b2355	0.22	+0.3
2,469,751	-	yfdL	b2355	0.32	-0.8
2,469,754	-	yfdL	b2355	0.32	-0.4
2,469,758	-	yfdL	b2355	0.33	+1.0
2,469,763	-	yfdL	b2355	0.34	-0.4
2,469,763	-	yfdL	b2355	0.34	-0.3
2,469,763	-	yfdL	b2355	0.34	-0.2
2,469,763	-	yfdL	b2355	0.34	+0.8
2,469,763	-	yfdL	b2355	0.34	-0.9
2,469,763	-	yfdL	b2355	0.34	-0.5
2,469,772	+	yfdL	b2355	0.36	+1.6
2,469,801	-	yfdL	b2355	0.41	+0.5
2,469,852	-	yfdL	b2355	0.49	+0.2
2,469,852	-	yfdL	b2355	0.49	+0.4
2,469,857	+	yfdL	b2355	0.50	+1.6
2,469,865	+	yfdL	b2355	0.52	+0.6
2,469,867	-	yfdL	b2355	0.52	-0.2
2,469,890	-	yfdL	b2355	0.56	+0.1
2,469,940	-	yfdL	b2355	0.65	+0.4
2,469,942	-	yfdL	b2355	0.65	+0.2
2,469,942	-	yfdL	b2355	0.65	-0.0
2,469,998	+	yfdL	b2355	0.75	+1.2
2,470,038	-	yfdL	b2355	0.82	-0.6
2,470,038	-	yfdL	b2355	0.82	-0.2
2,470,049	-	yfdL	b2355	0.83	+1.4
2,470,049	-	yfdL	b2355	0.83	-0.6
2,470,090	-				-0.1
2,470,168	+	yfdM	b2356	0.10	+1.3
2,470,211	-	yfdM	b2356	0.26	+0.1
2,470,448	+				-0.0
2,470,448	+				+1.2
2,470,477	+	yfdN	b2357	0.14	-0.6
2,470,477	+	yfdN	b2357	0.14	-0.7
2,470,484	-	yfdN	b2357	0.15	-0.5
2,470,484	-	yfdN	b2357	0.15	-0.3