Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrdB and yfaE overlap by 1 nucleotides yfaE and inaA are separated by 53 nucleotides inaA and yfaH are separated by 178 nucleotides yfaH and glpQ are separated by 41 nucleotides
b2235: nrdB - ribonucleotide-diphosphate reductase beta subunit (NCBI), at 2,345,406 to 2,346,536
nrdB
b2236: yfaE - predicted 2Fe-2S cluster-containing protein (NCBI), at 2,346,536 to 2,346,790
yfaE
b2237: inaA - hypothetical protein (NCBI), at 2,346,844 to 2,347,494
inaA
b2238: yfaH - hypothetical protein (RefSeq), at 2,347,673 to 2,347,915
yfaH
b2239: glpQ - periplasmic glycerophosphodiester phosphodiesterase (NCBI), at 2,347,957 to 2,349,033
glpQ
Position (kb)
2346
2347
2348 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2346.543 kb on + strand at 2346.585 kb on - strand, within yfaE at 2346.626 kb on - strand, within yfaE at 2346.721 kb on - strand, within yfaE at 2346.851 kb on + strand at 2346.976 kb on - strand, within inaA at 2347.083 kb on + strand, within inaA at 2347.130 kb on + strand, within inaA at 2347.184 kb on + strand, within inaA at 2347.346 kb on + strand, within inaA at 2347.361 kb on - strand, within inaA at 2347.608 kb on - strand at 2347.641 kb on - strand at 2347.641 kb on - strand at 2347.653 kb on + strand at 2347.855 kb on - strand, within yfaH at 2347.949 kb on - strand at 2347.955 kb on + strand at 2348.019 kb on + strand at 2348.055 kb on + strand at 2348.084 kb on - strand, within glpQ at 2348.084 kb on - strand, within glpQ at 2348.093 kb on + strand, within glpQ at 2348.099 kb on - strand, within glpQ at 2348.101 kb on - strand, within glpQ at 2348.101 kb on - strand, within glpQ at 2348.101 kb on - strand, within glpQ at 2348.101 kb on - strand, within glpQ at 2348.101 kb on - strand, within glpQ at 2348.137 kb on - strand, within glpQ at 2348.149 kb on - strand, within glpQ at 2348.149 kb on - strand, within glpQ at 2348.153 kb on + strand, within glpQ at 2348.211 kb on + strand, within glpQ at 2348.219 kb on - strand, within glpQ at 2348.338 kb on - strand, within glpQ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 2,346,543 + -0.0 2,346,585 - yfaE b2236 0.19 +0.1 2,346,626 - yfaE b2236 0.35 +0.3 2,346,721 - yfaE b2236 0.73 -0.8 2,346,851 + -1.0 2,346,976 - inaA b2237 0.20 +0.5 2,347,083 + inaA b2237 0.37 -0.8 2,347,130 + inaA b2237 0.44 -0.2 2,347,184 + inaA b2237 0.52 -1.7 2,347,346 + inaA b2237 0.77 -0.1 2,347,361 - inaA b2237 0.79 -0.8 2,347,608 - -0.3 2,347,641 - +2.5 2,347,641 - +0.1 2,347,653 + +0.0 2,347,855 - yfaH b2238 0.75 +2.5 2,347,949 - -1.0 2,347,955 + +0.6 2,348,019 + +0.9 2,348,055 + +0.2 2,348,084 - glpQ b2239 0.12 -1.0 2,348,084 - glpQ b2239 0.12 +0.4 2,348,093 + glpQ b2239 0.13 -0.5 2,348,099 - glpQ b2239 0.13 -2.0 2,348,101 - glpQ b2239 0.13 -0.1 2,348,101 - glpQ b2239 0.13 +1.0 2,348,101 - glpQ b2239 0.13 -0.3 2,348,101 - glpQ b2239 0.13 -2.1 2,348,101 - glpQ b2239 0.13 -0.5 2,348,137 - glpQ b2239 0.17 +0.5 2,348,149 - glpQ b2239 0.18 -0.9 2,348,149 - glpQ b2239 0.18 +0.4 2,348,153 + glpQ b2239 0.18 -0.4 2,348,211 + glpQ b2239 0.24 +0.8 2,348,219 - glpQ b2239 0.24 +1.0 2,348,338 - glpQ b2239 0.35 -0.7
Or see this region's nucleotide sequence