Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yejK and yejL are separated by 181 nucleotides yejL and yejM are separated by 19 nucleotides yejM and proL are separated by 74 nucleotides proL and yejO are separated by 102 nucleotides
b2186: yejK - nucleoid-associated protein NdpA (NCBI), at 2,280,962 to 2,281,969
yejK
b2187: yejL - hypothetical protein (NCBI), at 2,282,151 to 2,282,378
yejL
b2188: yejM - predicted hydrolase, inner membrane (NCBI), at 2,282,398 to 2,284,158
yejM
b2189: proL - tRNA-Pro (NCBI), at 2,284,233 to 2,284,309
proL
b2190: yejO - predicted autotransporter outer membrane protein (RefSeq), at 2,284,412 to 2,286,936
yejO
Position (kb)
2282
2283
2284
2285 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2281.447 kb on + strand, within yejK at 2281.456 kb on - strand, within yejK at 2281.483 kb on + strand, within yejK at 2281.512 kb on - strand, within yejK at 2281.512 kb on - strand, within yejK at 2281.780 kb on - strand, within yejK at 2281.887 kb on + strand at 2281.905 kb on - strand at 2282.004 kb on - strand at 2282.026 kb on + strand at 2282.026 kb on + strand at 2282.256 kb on + strand, within yejL at 2282.304 kb on + strand, within yejL at 2283.015 kb on + strand, within yejM at 2283.060 kb on + strand, within yejM at 2283.103 kb on - strand, within yejM at 2283.140 kb on - strand, within yejM at 2283.162 kb on + strand, within yejM at 2283.194 kb on - strand, within yejM at 2283.202 kb on + strand, within yejM at 2283.202 kb on + strand, within yejM at 2283.223 kb on + strand, within yejM at 2283.223 kb on + strand, within yejM at 2283.226 kb on + strand, within yejM at 2283.279 kb on + strand, within yejM at 2283.304 kb on - strand, within yejM at 2283.324 kb on + strand, within yejM at 2283.324 kb on + strand, within yejM at 2283.395 kb on + strand, within yejM at 2283.395 kb on + strand, within yejM at 2283.409 kb on - strand, within yejM at 2283.474 kb on - strand, within yejM at 2283.688 kb on + strand, within yejM at 2283.688 kb on + strand, within yejM at 2283.712 kb on - strand, within yejM at 2283.752 kb on + strand, within yejM at 2283.752 kb on + strand, within yejM at 2283.752 kb on + strand, within yejM at 2283.758 kb on - strand, within yejM at 2283.760 kb on - strand, within yejM at 2283.789 kb on + strand, within yejM at 2283.797 kb on + strand, within yejM at 2283.875 kb on + strand, within yejM at 2283.875 kb on + strand, within yejM at 2283.900 kb on - strand, within yejM at 2283.910 kb on - strand, within yejM at 2283.917 kb on - strand, within yejM at 2283.980 kb on - strand, within yejM at 2284.005 kb on + strand at 2284.005 kb on + strand at 2284.005 kb on + strand at 2284.051 kb on - strand at 2284.051 kb on - strand at 2284.056 kb on - strand at 2284.205 kb on + strand at 2284.255 kb on - strand, within proL at 2284.384 kb on - strand at 2284.395 kb on - strand at 2284.454 kb on + strand at 2284.454 kb on + strand at 2284.462 kb on - strand at 2284.479 kb on - strand at 2284.506 kb on + strand at 2284.528 kb on - strand at 2284.528 kb on - strand at 2284.606 kb on - strand at 2284.606 kb on - strand at 2284.667 kb on + strand, within yejO at 2284.704 kb on + strand, within yejO at 2284.729 kb on - strand, within yejO at 2284.729 kb on - strand, within yejO at 2284.748 kb on - strand, within yejO at 2284.755 kb on - strand, within yejO at 2284.764 kb on - strand, within yejO at 2284.764 kb on - strand, within yejO at 2284.795 kb on - strand, within yejO at 2284.795 kb on - strand, within yejO at 2284.796 kb on - strand, within yejO at 2284.796 kb on - strand, within yejO at 2284.932 kb on + strand, within yejO at 2284.982 kb on - strand, within yejO at 2284.982 kb on - strand, within yejO at 2284.993 kb on + strand, within yejO at 2285.000 kb on + strand, within yejO at 2285.001 kb on - strand, within yejO at 2285.126 kb on + strand, within yejO
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 2,281,447 + yejK b2186 0.48 +0.1 2,281,456 - yejK b2186 0.49 -0.5 2,281,483 + yejK b2186 0.52 -0.2 2,281,512 - yejK b2186 0.55 -0.9 2,281,512 - yejK b2186 0.55 -0.4 2,281,780 - yejK b2186 0.81 -0.2 2,281,887 + -0.7 2,281,905 - +1.0 2,282,004 - -0.8 2,282,026 + -0.2 2,282,026 + -0.8 2,282,256 + yejL b2187 0.46 +0.4 2,282,304 + yejL b2187 0.67 +0.2 2,283,015 + yejM b2188 0.35 +1.4 2,283,060 + yejM b2188 0.38 -0.2 2,283,103 - yejM b2188 0.40 -0.2 2,283,140 - yejM b2188 0.42 -1.0 2,283,162 + yejM b2188 0.43 -0.6 2,283,194 - yejM b2188 0.45 -0.2 2,283,202 + yejM b2188 0.46 -2.0 2,283,202 + yejM b2188 0.46 -0.8 2,283,223 + yejM b2188 0.47 -0.6 2,283,223 + yejM b2188 0.47 -0.6 2,283,226 + yejM b2188 0.47 -1.2 2,283,279 + yejM b2188 0.50 -0.9 2,283,304 - yejM b2188 0.51 -2.0 2,283,324 + yejM b2188 0.53 +0.3 2,283,324 + yejM b2188 0.53 -1.5 2,283,395 + yejM b2188 0.57 -1.2 2,283,395 + yejM b2188 0.57 -1.0 2,283,409 - yejM b2188 0.57 +0.2 2,283,474 - yejM b2188 0.61 +0.0 2,283,688 + yejM b2188 0.73 -2.8 2,283,688 + yejM b2188 0.73 -1.4 2,283,712 - yejM b2188 0.75 -1.2 2,283,752 + yejM b2188 0.77 -1.4 2,283,752 + yejM b2188 0.77 -0.9 2,283,752 + yejM b2188 0.77 -1.4 2,283,758 - yejM b2188 0.77 -0.7 2,283,760 - yejM b2188 0.77 -1.4 2,283,789 + yejM b2188 0.79 +0.2 2,283,797 + yejM b2188 0.79 -1.0 2,283,875 + yejM b2188 0.84 -0.8 2,283,875 + yejM b2188 0.84 -1.5 2,283,900 - yejM b2188 0.85 -0.6 2,283,910 - yejM b2188 0.86 -1.4 2,283,917 - yejM b2188 0.86 +0.4 2,283,980 - yejM b2188 0.90 -0.3 2,284,005 + +0.7 2,284,005 + -0.3 2,284,005 + +1.1 2,284,051 - -0.4 2,284,051 - -0.6 2,284,056 - -1.2 2,284,205 + -0.6 2,284,255 - proL b2189 0.29 -0.5 2,284,384 - -1.2 2,284,395 - -0.9 2,284,454 + -1.6 2,284,454 + -1.7 2,284,462 - -0.4 2,284,479 - -0.6 2,284,506 + -0.2 2,284,528 - -0.2 2,284,528 - +0.2 2,284,606 - -0.9 2,284,606 - -0.3 2,284,667 + yejO b2190 0.10 +1.4 2,284,704 + yejO b2190 0.12 +0.4 2,284,729 - yejO b2190 0.13 +0.1 2,284,729 - yejO b2190 0.13 -0.7 2,284,748 - yejO b2190 0.13 +0.1 2,284,755 - yejO b2190 0.14 -0.1 2,284,764 - yejO b2190 0.14 -0.9 2,284,764 - yejO b2190 0.14 -0.2 2,284,795 - yejO b2190 0.15 -0.6 2,284,795 - yejO b2190 0.15 +0.0 2,284,796 - yejO b2190 0.15 -0.4 2,284,796 - yejO b2190 0.15 +0.2 2,284,932 + yejO b2190 0.21 +0.3 2,284,982 - yejO b2190 0.23 +0.4 2,284,982 - yejO b2190 0.23 +1.0 2,284,993 + yejO b2190 0.23 +0.5 2,285,000 + yejO b2190 0.23 -0.3 2,285,001 - yejO b2190 0.23 +0.6 2,285,126 + yejO b2190 0.28 +0.3
Or see this region's nucleotide sequence