Strain Fitness in Escherichia coli BW25113 around b2068

Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegE and alkA are separated by 108 nucleotidesalkA and yegD are separated by 133 nucleotides b2067: yegE - predicted diguanylate cyclase, GGDEF domain signalling protein (NCBI), at 2,141,290 to 2,144,607 yegE b2068: alkA - 3-methyl-adenine DNA glycosylase II (NCBI), at 2,144,716 to 2,145,564 alkA b2069: yegD - putative heat shock protein (VIMSS), at 2,145,698 to 2,147,050 yegD Position (kb) 2144 2145 2146Strain fitness (log2 ratio) -1 0 1at 2143.858 kb on - strand, within yegEat 2143.862 kb on + strand, within yegEat 2143.862 kb on + strand, within yegEat 2143.864 kb on + strand, within yegEat 2143.894 kb on + strand, within yegEat 2143.894 kb on + strand, within yegEat 2144.000 kb on - strand, within yegEat 2144.201 kb on + strand, within yegEat 2144.299 kb on + strandat 2144.299 kb on + strandat 2144.383 kb on - strandat 2144.501 kb on + strandat 2144.614 kb on + strandat 2144.721 kb on - strandat 2144.721 kb on - strandat 2144.753 kb on + strandat 2144.877 kb on + strand, within alkAat 2144.963 kb on - strand, within alkAat 2145.185 kb on + strand, within alkAat 2145.267 kb on + strand, within alkAat 2145.336 kb on + strand, within alkAat 2145.336 kb on + strand, within alkAat 2145.398 kb on - strand, within alkAat 2145.398 kb on - strand, within alkAat 2145.398 kb on - strand, within alkAat 2145.398 kb on - strand, within alkAat 2145.452 kb on - strand, within alkAat 2145.480 kb on + strandat 2145.540 kb on - strandat 2145.557 kb on + strandat 2145.565 kb on - strandat 2145.721 kb on - strandat 2145.726 kb on - strandat 2145.726 kb on - strandat 2145.794 kb on + strandat 2145.794 kb on + strandat 2145.911 kb on + strand, within yegDat 2145.936 kb on + strand, within yegDat 2146.210 kb on + strand, within yegDat 2146.262 kb on + strand, within yegDat 2146.348 kb on - strand, within yegDat 2146.351 kb on + strand, within yegDat 2146.351 kb on + strand, within yegDat 2146.359 kb on - strand, within yegDat 2146.361 kb on - strand, within yegDat 2146.361 kb on - strand, within yegDat 2146.368 kb on + strand, within yegDat 2146.368 kb on + strand, within yegDat 2146.538 kb on - strand, within yegD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM
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2,143,858 - yegE b2067 0.77 +0.4
2,143,862 + yegE b2067 0.78 +0.7
2,143,862 + yegE b2067 0.78 +1.4
2,143,864 + yegE b2067 0.78 +0.1
2,143,894 + yegE b2067 0.78 -0.5
2,143,894 + yegE b2067 0.78 +0.7
2,144,000 - yegE b2067 0.82 +0.6
2,144,201 + yegE b2067 0.88 +0.6
2,144,299 + -0.0
2,144,299 + +0.4
2,144,383 - +1.4
2,144,501 + -0.1
2,144,614 + -0.5
2,144,721 - -0.1
2,144,721 - -0.3
2,144,753 + -1.3
2,144,877 + alkA b2068 0.19 +0.2
2,144,963 - alkA b2068 0.29 -0.0
2,145,185 + alkA b2068 0.55 -0.3
2,145,267 + alkA b2068 0.65 +0.8
2,145,336 + alkA b2068 0.73 +0.4
2,145,336 + alkA b2068 0.73 -0.5
2,145,398 - alkA b2068 0.80 +0.3
2,145,398 - alkA b2068 0.80 -0.8
2,145,398 - alkA b2068 0.80 +0.8
2,145,398 - alkA b2068 0.80 -0.0
2,145,452 - alkA b2068 0.87 -0.8
2,145,480 + -0.5
2,145,540 - +0.3
2,145,557 + -0.4
2,145,565 - -0.3
2,145,721 - -0.4
2,145,726 - +0.2
2,145,726 - -0.6
2,145,794 + +0.1
2,145,794 + -0.3
2,145,911 + yegD b2069 0.16 -0.2
2,145,936 + yegD b2069 0.18 -0.2
2,146,210 + yegD b2069 0.38 -0.3
2,146,262 + yegD b2069 0.42 -1.2
2,146,348 - yegD b2069 0.48 +0.2
2,146,351 + yegD b2069 0.48 -0.1
2,146,351 + yegD b2069 0.48 -0.2
2,146,359 - yegD b2069 0.49 +0.1
2,146,361 - yegD b2069 0.49 -0.1
2,146,361 - yegD b2069 0.49 +0.1
2,146,368 + yegD b2069 0.50 -0.5
2,146,368 + yegD b2069 0.50 -0.1
2,146,538 - yegD b2069 0.62 +1.1

Or see this region's nucleotide sequence