Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dacD and sbcB are separated by 208 nucleotides sbcB and yeeD are separated by 42 nucleotides yeeD and yeeE are separated by 13 nucleotides
b2010: dacD - penicillin binding protein 6b (VIMSS), at 2,079,405 to 2,080,571
dacD
b2011: sbcB - exonuclease I (NCBI), at 2,080,780 to 2,082,207
sbcB
b2012: yeeD - hypothetical protein (NCBI), at 2,082,250 to 2,082,477
yeeD
b2013: yeeE - predicted inner membrane protein (NCBI), at 2,082,491 to 2,083,549
yeeE
Position (kb)
2080
2081
2082
2083 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2079.784 kb on + strand, within dacD at 2079.833 kb on - strand, within dacD at 2079.833 kb on - strand, within dacD at 2079.857 kb on + strand, within dacD at 2079.857 kb on + strand, within dacD at 2079.966 kb on + strand, within dacD at 2080.001 kb on + strand, within dacD at 2080.041 kb on + strand, within dacD at 2080.420 kb on + strand, within dacD at 2080.420 kb on + strand, within dacD at 2080.685 kb on - strand at 2080.685 kb on - strand at 2080.691 kb on - strand at 2080.744 kb on + strand at 2080.777 kb on - strand at 2080.787 kb on - strand at 2080.857 kb on - strand at 2080.857 kb on - strand at 2080.867 kb on - strand at 2080.867 kb on - strand at 2080.982 kb on + strand, within sbcB at 2080.982 kb on + strand, within sbcB at 2080.990 kb on - strand, within sbcB at 2081.062 kb on + strand, within sbcB at 2081.062 kb on + strand, within sbcB at 2081.077 kb on - strand, within sbcB at 2081.151 kb on + strand, within sbcB at 2081.153 kb on + strand, within sbcB at 2081.218 kb on - strand, within sbcB at 2081.220 kb on + strand, within sbcB at 2081.238 kb on - strand, within sbcB at 2081.289 kb on - strand, within sbcB at 2081.289 kb on - strand, within sbcB at 2081.300 kb on + strand, within sbcB at 2081.307 kb on + strand, within sbcB at 2081.308 kb on - strand, within sbcB at 2081.308 kb on - strand, within sbcB at 2081.410 kb on - strand, within sbcB at 2081.411 kb on + strand, within sbcB at 2081.411 kb on + strand, within sbcB at 2081.513 kb on + strand, within sbcB at 2081.518 kb on - strand, within sbcB at 2081.625 kb on + strand, within sbcB at 2081.675 kb on + strand, within sbcB at 2081.679 kb on + strand, within sbcB at 2081.688 kb on - strand, within sbcB at 2081.701 kb on + strand, within sbcB at 2081.862 kb on - strand, within sbcB at 2081.862 kb on - strand, within sbcB at 2081.885 kb on - strand, within sbcB at 2082.093 kb on + strand at 2082.093 kb on + strand at 2082.168 kb on + strand at 2082.266 kb on + strand at 2082.266 kb on + strand at 2082.266 kb on + strand at 2082.299 kb on + strand, within yeeD at 2082.299 kb on + strand, within yeeD at 2082.429 kb on - strand, within yeeD at 2082.537 kb on - strand at 2082.537 kb on - strand at 2082.544 kb on - strand at 2082.544 kb on - strand at 2082.546 kb on + strand at 2082.674 kb on + strand, within yeeE at 2082.694 kb on - strand, within yeeE at 2082.775 kb on - strand, within yeeE at 2082.775 kb on - strand, within yeeE at 2082.851 kb on - strand, within yeeE at 2082.881 kb on + strand, within yeeE at 2082.897 kb on - strand, within yeeE at 2082.897 kb on - strand, within yeeE at 2082.915 kb on - strand, within yeeE at 2082.973 kb on + strand, within yeeE at 2082.973 kb on + strand, within yeeE at 2082.981 kb on - strand, within yeeE at 2083.040 kb on - strand, within yeeE at 2083.040 kb on - strand, within yeeE at 2083.126 kb on + strand, within yeeE at 2083.139 kb on + strand, within yeeE at 2083.183 kb on + strand, within yeeE at 2083.186 kb on - strand, within yeeE at 2083.186 kb on - strand, within yeeE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 2,079,784 + dacD b2010 0.32 -2.6 2,079,833 - dacD b2010 0.37 -0.7 2,079,833 - dacD b2010 0.37 +0.0 2,079,857 + dacD b2010 0.39 -0.4 2,079,857 + dacD b2010 0.39 -0.6 2,079,966 + dacD b2010 0.48 -0.4 2,080,001 + dacD b2010 0.51 -0.2 2,080,041 + dacD b2010 0.54 +0.8 2,080,420 + dacD b2010 0.87 +1.6 2,080,420 + dacD b2010 0.87 +1.4 2,080,685 - +0.2 2,080,685 - -0.3 2,080,691 - -0.5 2,080,744 + +0.5 2,080,777 - -0.3 2,080,787 - -0.8 2,080,857 - -0.9 2,080,857 - +0.4 2,080,867 - -2.5 2,080,867 - -1.3 2,080,982 + sbcB b2011 0.14 -0.8 2,080,982 + sbcB b2011 0.14 -0.9 2,080,990 - sbcB b2011 0.15 -0.3 2,081,062 + sbcB b2011 0.20 -1.5 2,081,062 + sbcB b2011 0.20 -1.2 2,081,077 - sbcB b2011 0.21 -0.2 2,081,151 + sbcB b2011 0.26 -0.5 2,081,153 + sbcB b2011 0.26 -1.5 2,081,218 - sbcB b2011 0.31 -1.0 2,081,220 + sbcB b2011 0.31 -1.3 2,081,238 - sbcB b2011 0.32 -0.8 2,081,289 - sbcB b2011 0.36 -0.0 2,081,289 - sbcB b2011 0.36 -1.2 2,081,300 + sbcB b2011 0.36 -1.2 2,081,307 + sbcB b2011 0.37 -0.3 2,081,308 - sbcB b2011 0.37 -0.7 2,081,308 - sbcB b2011 0.37 -0.0 2,081,410 - sbcB b2011 0.44 -3.1 2,081,411 + sbcB b2011 0.44 +0.7 2,081,411 + sbcB b2011 0.44 -0.5 2,081,513 + sbcB b2011 0.51 -2.3 2,081,518 - sbcB b2011 0.52 -1.1 2,081,625 + sbcB b2011 0.59 -1.1 2,081,675 + sbcB b2011 0.63 +0.1 2,081,679 + sbcB b2011 0.63 -0.6 2,081,688 - sbcB b2011 0.64 +0.4 2,081,701 + sbcB b2011 0.64 -1.3 2,081,862 - sbcB b2011 0.76 -0.5 2,081,862 - sbcB b2011 0.76 -0.9 2,081,885 - sbcB b2011 0.77 -0.4 2,082,093 + -1.8 2,082,093 + -0.9 2,082,168 + -0.1 2,082,266 + +0.1 2,082,266 + -1.0 2,082,266 + -0.5 2,082,299 + yeeD b2012 0.21 -1.6 2,082,299 + yeeD b2012 0.21 +0.5 2,082,429 - yeeD b2012 0.79 -0.2 2,082,537 - -0.6 2,082,537 - -0.5 2,082,544 - -0.2 2,082,544 - +0.1 2,082,546 + +0.3 2,082,674 + yeeE b2013 0.17 +0.1 2,082,694 - yeeE b2013 0.19 -0.4 2,082,775 - yeeE b2013 0.27 -2.3 2,082,775 - yeeE b2013 0.27 -1.6 2,082,851 - yeeE b2013 0.34 -0.4 2,082,881 + yeeE b2013 0.37 -0.4 2,082,897 - yeeE b2013 0.38 +0.6 2,082,897 - yeeE b2013 0.38 -0.7 2,082,915 - yeeE b2013 0.40 -0.2 2,082,973 + yeeE b2013 0.46 -1.4 2,082,973 + yeeE b2013 0.46 -1.1 2,082,981 - yeeE b2013 0.46 +0.1 2,083,040 - yeeE b2013 0.52 -0.2 2,083,040 - yeeE b2013 0.52 -0.3 2,083,126 + yeeE b2013 0.60 -0.2 2,083,139 + yeeE b2013 0.61 -1.0 2,083,183 + yeeE b2013 0.65 -1.6 2,083,186 - yeeE b2013 0.66 +0.5 2,083,186 - yeeE b2013 0.66 +0.6
Or see this region's nucleotide sequence