Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhS and ydhT are separated by 11 nucleotides ydhT and ydhU are separated by 3 nucleotides ydhU and ydhX overlap by 4 nucleotides ydhX and ydhW are separated by 63 nucleotides
b1668: ydhS - conserved protein with FAD/NAD(P)-binding domain (NCBI), at 1,745,155 to 1,746,759
ydhS
b1669: ydhT - hypothetical protein (NCBI), at 1,746,771 to 1,747,583
ydhT
b1670: ydhU - predicted cytochrome (NCBI), at 1,747,587 to 1,748,372
ydhU
b1671: ydhX - predicted 4Fe-4S ferridoxin-type protein (RefSeq), at 1,748,369 to 1,749,037
ydhX
b1672: ydhW - hypothetical protein (NCBI), at 1,749,101 to 1,749,748
ydhW
Position (kb)
1747
1748
1749 Strain fitness (log2 ratio)
-2
-1
0
1 at 1746.854 kb on - strand, within ydhT at 1746.877 kb on + strand, within ydhT at 1746.969 kb on + strand, within ydhT at 1747.895 kb on - strand, within ydhU at 1747.958 kb on - strand, within ydhU at 1747.958 kb on - strand, within ydhU at 1747.965 kb on - strand, within ydhU at 1748.076 kb on - strand, within ydhU at 1748.231 kb on - strand, within ydhU at 1748.231 kb on - strand, within ydhU at 1748.764 kb on - strand, within ydhX at 1748.764 kb on - strand, within ydhX at 1748.847 kb on - strand, within ydhX at 1749.057 kb on - strand at 1749.057 kb on - strand at 1749.123 kb on + strand at 1749.144 kb on + strand at 1749.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,746,854 - ydhT b1669 0.10 -0.2 1,746,877 + ydhT b1669 0.13 -0.9 1,746,969 + ydhT b1669 0.24 -1.1 1,747,895 - ydhU b1670 0.39 +0.1 1,747,958 - ydhU b1670 0.47 -0.6 1,747,958 - ydhU b1670 0.47 -1.1 1,747,965 - ydhU b1670 0.48 -0.4 1,748,076 - ydhU b1670 0.62 -0.6 1,748,231 - ydhU b1670 0.82 -2.2 1,748,231 - ydhU b1670 0.82 -0.8 1,748,764 - ydhX b1671 0.59 -0.2 1,748,764 - ydhX b1671 0.59 -0.6 1,748,847 - ydhX b1671 0.71 +1.1 1,749,057 - -0.3 1,749,057 - -0.0 1,749,123 + +0.2 1,749,144 + -1.6 1,749,151 - -0.8
Or see this region's nucleotide sequence