Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsxG and rsxE are separated by 3 nucleotides rsxE and nth overlap by 1 nucleotides nth and tppB are separated by 610 nucleotides
b1631: rsxG - electron transport complex protein RnfG (NCBI), at 1,708,228 to 1,708,848
rsxG
b1632: rsxE - NADH-ubiquinone oxidoreductase (NCBI), at 1,708,852 to 1,709,547
rsxE
b1633: nth - DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI), at 1,709,547 to 1,710,182
nth
b1634: tppB - putative tripeptide transporter permease (NCBI), at 1,710,793 to 1,712,295
tppB
Position (kb)
1709
1710
1711 Strain fitness (log2 ratio)
-2
-1
0
1 at 1708.996 kb on - strand, within rsxE at 1709.004 kb on + strand, within rsxE at 1709.034 kb on - strand, within rsxE at 1709.096 kb on + strand, within rsxE at 1709.096 kb on + strand, within rsxE at 1709.106 kb on - strand, within rsxE at 1709.123 kb on + strand, within rsxE at 1709.128 kb on + strand, within rsxE at 1709.182 kb on + strand, within rsxE at 1709.254 kb on + strand, within rsxE at 1709.303 kb on - strand, within rsxE at 1709.320 kb on + strand, within rsxE at 1709.330 kb on - strand, within rsxE at 1709.330 kb on - strand, within rsxE at 1709.393 kb on + strand, within rsxE at 1709.445 kb on + strand, within rsxE at 1709.464 kb on - strand, within rsxE at 1709.517 kb on - strand at 1709.581 kb on + strand at 1709.581 kb on + strand at 1709.691 kb on - strand, within nth at 1709.760 kb on - strand, within nth at 1709.843 kb on + strand, within nth at 1709.859 kb on + strand, within nth at 1709.859 kb on + strand, within nth at 1709.876 kb on + strand, within nth at 1709.876 kb on + strand, within nth at 1710.159 kb on + strand at 1710.274 kb on - strand at 1710.551 kb on - strand at 1710.837 kb on - strand at 1710.838 kb on - strand at 1710.870 kb on - strand at 1711.174 kb on - strand, within tppB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,708,996 - rsxE b1632 0.21 +1.1 1,709,004 + rsxE b1632 0.22 +1.5 1,709,034 - rsxE b1632 0.26 +1.0 1,709,096 + rsxE b1632 0.35 +0.5 1,709,096 + rsxE b1632 0.35 +1.1 1,709,106 - rsxE b1632 0.36 +0.5 1,709,123 + rsxE b1632 0.39 +1.1 1,709,128 + rsxE b1632 0.40 +1.0 1,709,182 + rsxE b1632 0.47 +0.8 1,709,254 + rsxE b1632 0.58 +0.4 1,709,303 - rsxE b1632 0.65 +1.4 1,709,320 + rsxE b1632 0.67 +1.4 1,709,330 - rsxE b1632 0.69 +0.6 1,709,330 - rsxE b1632 0.69 +0.7 1,709,393 + rsxE b1632 0.78 +0.6 1,709,445 + rsxE b1632 0.85 -0.4 1,709,464 - rsxE b1632 0.88 -1.3 1,709,517 - +0.2 1,709,581 + -0.5 1,709,581 + -1.1 1,709,691 - nth b1633 0.23 +0.8 1,709,760 - nth b1633 0.33 +0.4 1,709,843 + nth b1633 0.47 +0.4 1,709,859 + nth b1633 0.49 -0.1 1,709,859 + nth b1633 0.49 +0.0 1,709,876 + nth b1633 0.52 -1.9 1,709,876 + nth b1633 0.52 +0.9 1,710,159 + +1.0 1,710,274 - -0.1 1,710,551 - +0.3 1,710,837 - -1.5 1,710,838 - +0.5 1,710,870 - +0.3 1,711,174 - tppB b1634 0.25 -0.6
Or see this region's nucleotide sequence