Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pqqL and yddB are separated by 44 nucleotides yddB and yddA are separated by 37 nucleotides
b1494: pqqL - predicted peptidase (NCBI), at 1,570,431 to 1,573,226
pqqL
b1495: yddB - predicted porin protein (NCBI), at 1,573,271 to 1,575,643
yddB
b1496: yddA - fused predicted multidrug transporter subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 1,575,681 to 1,577,366
yddA
Position (kb)
1573
1574
1575
1576 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1572.286 kb on - strand, within pqqL at 1572.507 kb on + strand, within pqqL at 1572.546 kb on + strand, within pqqL at 1572.619 kb on + strand, within pqqL at 1572.619 kb on + strand, within pqqL at 1572.686 kb on + strand, within pqqL at 1572.789 kb on - strand, within pqqL at 1572.789 kb on - strand, within pqqL at 1572.792 kb on - strand, within pqqL at 1572.876 kb on + strand, within pqqL at 1572.877 kb on + strand, within pqqL at 1572.882 kb on + strand, within pqqL at 1572.882 kb on + strand, within pqqL at 1572.944 kb on - strand, within pqqL at 1573.008 kb on - strand at 1573.066 kb on - strand at 1573.066 kb on - strand at 1573.074 kb on - strand at 1573.074 kb on - strand at 1573.084 kb on - strand at 1573.193 kb on + strand at 1573.278 kb on - strand at 1573.297 kb on + strand at 1573.311 kb on + strand at 1573.322 kb on - strand at 1573.361 kb on + strand at 1573.439 kb on + strand at 1573.445 kb on + strand at 1573.445 kb on + strand at 1573.566 kb on + strand, within yddB at 1573.576 kb on + strand, within yddB at 1573.595 kb on + strand, within yddB at 1573.595 kb on + strand, within yddB at 1573.622 kb on + strand, within yddB at 1573.727 kb on + strand, within yddB at 1573.748 kb on + strand, within yddB at 1573.753 kb on + strand, within yddB at 1573.824 kb on - strand, within yddB at 1573.860 kb on + strand, within yddB at 1573.954 kb on - strand at 1573.965 kb on - strand, within yddB at 1573.990 kb on - strand, within yddB at 1574.031 kb on - strand, within yddB at 1574.031 kb on - strand, within yddB at 1574.063 kb on - strand, within yddB at 1574.072 kb on + strand, within yddB at 1574.074 kb on - strand, within yddB at 1574.271 kb on - strand, within yddB at 1574.277 kb on + strand, within yddB at 1574.282 kb on + strand, within yddB at 1574.285 kb on - strand, within yddB at 1574.372 kb on + strand, within yddB at 1574.381 kb on - strand, within yddB at 1574.383 kb on + strand, within yddB at 1574.392 kb on - strand, within yddB at 1574.392 kb on - strand, within yddB at 1574.392 kb on - strand, within yddB at 1574.409 kb on + strand, within yddB at 1574.443 kb on - strand, within yddB at 1574.516 kb on - strand, within yddB at 1574.540 kb on - strand, within yddB at 1574.554 kb on - strand, within yddB at 1574.647 kb on + strand, within yddB at 1574.647 kb on + strand, within yddB at 1574.670 kb on + strand, within yddB at 1574.846 kb on - strand, within yddB at 1574.898 kb on + strand, within yddB at 1574.906 kb on - strand, within yddB at 1574.929 kb on - strand, within yddB at 1575.055 kb on - strand, within yddB at 1575.055 kb on - strand, within yddB at 1575.063 kb on + strand, within yddB at 1575.064 kb on - strand, within yddB at 1575.069 kb on - strand, within yddB at 1575.132 kb on + strand, within yddB at 1575.132 kb on + strand, within yddB at 1575.153 kb on + strand, within yddB at 1575.226 kb on + strand, within yddB at 1575.243 kb on - strand, within yddB at 1575.259 kb on - strand, within yddB at 1575.261 kb on + strand, within yddB at 1575.269 kb on - strand, within yddB at 1575.283 kb on - strand, within yddB at 1575.283 kb on - strand, within yddB at 1575.296 kb on - strand, within yddB at 1575.362 kb on + strand, within yddB at 1575.416 kb on + strand at 1575.431 kb on + strand at 1575.432 kb on - strand at 1575.502 kb on - strand at 1575.529 kb on + strand at 1575.529 kb on + strand at 1575.532 kb on + strand at 1575.532 kb on + strand at 1575.542 kb on - strand at 1575.575 kb on - strand at 1575.646 kb on + strand at 1575.728 kb on - strand at 1575.733 kb on + strand at 1575.740 kb on - strand at 1575.742 kb on - strand at 1575.754 kb on - strand at 1575.754 kb on - strand at 1575.754 kb on - strand at 1575.754 kb on - strand at 1575.802 kb on - strand at 1575.802 kb on - strand at 1575.810 kb on - strand at 1575.880 kb on - strand, within yddA at 1575.944 kb on - strand, within yddA at 1575.964 kb on + strand, within yddA at 1575.966 kb on - strand, within yddA at 1575.977 kb on - strand, within yddA at 1575.996 kb on - strand, within yddA at 1576.039 kb on - strand, within yddA at 1576.066 kb on + strand, within yddA at 1576.070 kb on - strand, within yddA at 1576.110 kb on - strand, within yddA at 1576.110 kb on - strand, within yddA at 1576.122 kb on - strand, within yddA at 1576.245 kb on - strand, within yddA at 1576.260 kb on + strand, within yddA at 1576.310 kb on - strand, within yddA at 1576.313 kb on - strand, within yddA at 1576.327 kb on + strand, within yddA at 1576.459 kb on + strand, within yddA at 1576.497 kb on + strand, within yddA at 1576.617 kb on - strand, within yddA at 1576.629 kb on - strand, within yddA at 1576.629 kb on - strand, within yddA at 1576.629 kb on - strand, within yddA at 1576.629 kb on - strand, within yddA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,572,286 - pqqL b1494 0.66 +1.2 1,572,507 + pqqL b1494 0.74 +0.8 1,572,546 + pqqL b1494 0.76 +0.6 1,572,619 + pqqL b1494 0.78 +0.6 1,572,619 + pqqL b1494 0.78 +0.2 1,572,686 + pqqL b1494 0.81 +1.3 1,572,789 - pqqL b1494 0.84 +3.1 1,572,789 - pqqL b1494 0.84 +0.6 1,572,792 - pqqL b1494 0.84 +2.2 1,572,876 + pqqL b1494 0.87 +0.6 1,572,877 + pqqL b1494 0.87 +0.0 1,572,882 + pqqL b1494 0.88 -0.8 1,572,882 + pqqL b1494 0.88 +0.8 1,572,944 - pqqL b1494 0.90 -0.2 1,573,008 - -0.6 1,573,066 - +0.8 1,573,066 - -1.8 1,573,074 - +0.0 1,573,074 - +1.1 1,573,084 - -0.1 1,573,193 + +2.3 1,573,278 - -0.7 1,573,297 + -0.2 1,573,311 + -0.2 1,573,322 - -0.3 1,573,361 + -0.7 1,573,439 + +0.8 1,573,445 + +0.0 1,573,445 + +0.8 1,573,566 + yddB b1495 0.12 -0.8 1,573,576 + yddB b1495 0.13 -0.7 1,573,595 + yddB b1495 0.14 -0.2 1,573,595 + yddB b1495 0.14 +0.3 1,573,622 + yddB b1495 0.15 +0.5 1,573,727 + yddB b1495 0.19 -0.8 1,573,748 + yddB b1495 0.20 -1.2 1,573,753 + yddB b1495 0.20 +1.0 1,573,824 - yddB b1495 0.23 +0.9 1,573,860 + yddB b1495 0.25 -1.0 1,573,954 - -0.2 1,573,965 - yddB b1495 0.29 +1.3 1,573,990 - yddB b1495 0.30 -0.4 1,574,031 - yddB b1495 0.32 +0.1 1,574,031 - yddB b1495 0.32 +0.6 1,574,063 - yddB b1495 0.33 +0.5 1,574,072 + yddB b1495 0.34 +1.3 1,574,074 - yddB b1495 0.34 -0.2 1,574,271 - yddB b1495 0.42 -0.4 1,574,277 + yddB b1495 0.42 -2.4 1,574,282 + yddB b1495 0.43 -0.7 1,574,285 - yddB b1495 0.43 +0.2 1,574,372 + yddB b1495 0.46 -0.7 1,574,381 - yddB b1495 0.47 +0.3 1,574,383 + yddB b1495 0.47 -0.5 1,574,392 - yddB b1495 0.47 -0.7 1,574,392 - yddB b1495 0.47 -0.5 1,574,392 - yddB b1495 0.47 -0.8 1,574,409 + yddB b1495 0.48 -1.5 1,574,443 - yddB b1495 0.49 +1.3 1,574,516 - yddB b1495 0.52 -0.1 1,574,540 - yddB b1495 0.53 -0.7 1,574,554 - yddB b1495 0.54 -1.2 1,574,647 + yddB b1495 0.58 -1.0 1,574,647 + yddB b1495 0.58 -0.2 1,574,670 + yddB b1495 0.59 -0.7 1,574,846 - yddB b1495 0.66 +0.3 1,574,898 + yddB b1495 0.69 +1.3 1,574,906 - yddB b1495 0.69 -1.0 1,574,929 - yddB b1495 0.70 +1.3 1,575,055 - yddB b1495 0.75 -1.2 1,575,055 - yddB b1495 0.75 +0.8 1,575,063 + yddB b1495 0.76 +1.3 1,575,064 - yddB b1495 0.76 +0.1 1,575,069 - yddB b1495 0.76 +0.3 1,575,132 + yddB b1495 0.78 -0.2 1,575,132 + yddB b1495 0.78 -0.2 1,575,153 + yddB b1495 0.79 +0.3 1,575,226 + yddB b1495 0.82 +1.3 1,575,243 - yddB b1495 0.83 -0.2 1,575,259 - yddB b1495 0.84 -0.5 1,575,261 + yddB b1495 0.84 -0.2 1,575,269 - yddB b1495 0.84 -1.7 1,575,283 - yddB b1495 0.85 -1.8 1,575,283 - yddB b1495 0.85 -0.8 1,575,296 - yddB b1495 0.85 +0.8 1,575,362 + yddB b1495 0.88 +1.3 1,575,416 + +1.3 1,575,431 + -1.2 1,575,432 - -0.2 1,575,502 - +0.3 1,575,529 + -1.0 1,575,529 + -0.7 1,575,532 + +0.5 1,575,532 + -0.2 1,575,542 - +0.3 1,575,575 - +1.3 1,575,646 + +1.3 1,575,728 - -0.7 1,575,733 + +1.3 1,575,740 - +1.3 1,575,742 - -0.1 1,575,754 - -0.2 1,575,754 - +0.3 1,575,754 - -0.6 1,575,754 - +1.3 1,575,802 - +0.3 1,575,802 - +1.3 1,575,810 - -0.1 1,575,880 - yddA b1496 0.12 -0.2 1,575,944 - yddA b1496 0.16 -1.1 1,575,964 + yddA b1496 0.17 +1.3 1,575,966 - yddA b1496 0.17 -0.2 1,575,977 - yddA b1496 0.18 +2.0 1,575,996 - yddA b1496 0.19 -0.5 1,576,039 - yddA b1496 0.21 -2.0 1,576,066 + yddA b1496 0.23 +1.3 1,576,070 - yddA b1496 0.23 -0.7 1,576,110 - yddA b1496 0.25 +0.8 1,576,110 - yddA b1496 0.25 +1.3 1,576,122 - yddA b1496 0.26 +1.3 1,576,245 - yddA b1496 0.33 -0.2 1,576,260 + yddA b1496 0.34 +1.3 1,576,310 - yddA b1496 0.37 +1.3 1,576,313 - yddA b1496 0.37 +1.3 1,576,327 + yddA b1496 0.38 +1.3 1,576,459 + yddA b1496 0.46 +1.3 1,576,497 + yddA b1496 0.48 +1.3 1,576,617 - yddA b1496 0.56 +0.3 1,576,629 - yddA b1496 0.56 -0.2 1,576,629 - yddA b1496 0.56 +1.3 1,576,629 - yddA b1496 0.56 -1.5 1,576,629 - yddA b1496 0.56 +1.3
Or see this region's nucleotide sequence