Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kch and yciI are separated by 299 nucleotides yciI and tonB are separated by 223 nucleotides tonB and yciA are separated by 39 nucleotides
b1250: kch - voltage-gated potassium channel (NCBI), at 1,307,040 to 1,308,293
kch
b1251: yciI - orf, hypothetical protein (VIMSS), at 1,308,593 to 1,308,889
yciI
b1252: tonB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 1,309,113 to 1,309,832
tonB
b1253: yciA - predicted hydrolase (NCBI), at 1,309,872 to 1,310,270
yciA
Position (kb)
1308
1309 Strain fitness (log2 ratio)
-2
-1
0
1 at 1307.613 kb on + strand, within kch at 1307.613 kb on + strand, within kch at 1307.620 kb on - strand, within kch at 1307.820 kb on - strand, within kch at 1307.827 kb on - strand, within kch at 1307.895 kb on - strand, within kch at 1307.994 kb on - strand, within kch at 1307.994 kb on - strand, within kch at 1308.003 kb on + strand, within kch at 1308.045 kb on + strand, within kch at 1308.061 kb on - strand, within kch at 1308.061 kb on - strand, within kch at 1308.068 kb on + strand, within kch at 1308.103 kb on + strand, within kch at 1308.110 kb on + strand, within kch at 1308.256 kb on - strand at 1308.285 kb on + strand at 1308.298 kb on - strand at 1308.300 kb on - strand at 1308.380 kb on + strand at 1308.656 kb on - strand, within yciI at 1308.663 kb on - strand, within yciI at 1308.679 kb on - strand, within yciI at 1308.679 kb on - strand, within yciI at 1308.732 kb on + strand, within yciI at 1308.741 kb on - strand, within yciI at 1308.744 kb on + strand, within yciI at 1308.744 kb on + strand, within yciI at 1309.193 kb on + strand, within tonB at 1309.201 kb on - strand, within tonB at 1309.201 kb on - strand, within tonB at 1309.201 kb on - strand, within tonB at 1309.474 kb on + strand, within tonB at 1309.533 kb on - strand, within tonB at 1309.533 kb on - strand, within tonB at 1309.610 kb on + strand, within tonB at 1309.705 kb on - strand, within tonB at 1309.705 kb on - strand, within tonB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,307,613 + kch b1250 0.46 +0.9 1,307,613 + kch b1250 0.46 -0.7 1,307,620 - kch b1250 0.46 -0.8 1,307,820 - kch b1250 0.62 +0.5 1,307,827 - kch b1250 0.63 -0.3 1,307,895 - kch b1250 0.68 -0.3 1,307,994 - kch b1250 0.76 +0.2 1,307,994 - kch b1250 0.76 +0.6 1,308,003 + kch b1250 0.77 +1.5 1,308,045 + kch b1250 0.80 +0.5 1,308,061 - kch b1250 0.81 -1.9 1,308,061 - kch b1250 0.81 -0.1 1,308,068 + kch b1250 0.82 +1.5 1,308,103 + kch b1250 0.85 -1.1 1,308,110 + kch b1250 0.85 +1.5 1,308,256 - +0.1 1,308,285 + +0.8 1,308,298 - +1.5 1,308,300 - -0.1 1,308,380 + +1.5 1,308,656 - yciI b1251 0.21 -1.5 1,308,663 - yciI b1251 0.24 -1.1 1,308,679 - yciI b1251 0.29 -0.5 1,308,679 - yciI b1251 0.29 -0.1 1,308,732 + yciI b1251 0.47 -0.1 1,308,741 - yciI b1251 0.50 -1.5 1,308,744 + yciI b1251 0.51 +0.5 1,308,744 + yciI b1251 0.51 +0.3 1,309,193 + tonB b1252 0.11 -1.7 1,309,201 - tonB b1252 0.12 +0.5 1,309,201 - tonB b1252 0.12 +0.1 1,309,201 - tonB b1252 0.12 -0.8 1,309,474 + tonB b1252 0.50 -0.2 1,309,533 - tonB b1252 0.58 -0.1 1,309,533 - tonB b1252 0.58 -1.7 1,309,610 + tonB b1252 0.69 -1.0 1,309,705 - tonB b1252 0.82 -0.5 1,309,705 - tonB b1252 0.82 +0.3
Or see this region's nucleotide sequence