Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt oppD and oppF overlap by 4 nucleotides oppF and b1248 are separated by 52 nucleotides b1248 and cls are separated by 34 nucleotides
b1246: oppD - oligopeptide transporter ATP-binding component (NCBI), at 1,302,778 to 1,303,791
oppD
b1247: oppF - oligopeptide transporter subunit (NCBI), at 1,303,788 to 1,304,792
oppF
b1248: b1248 - orf, hypothetical protein (VIMSS), at 1,304,845 to 1,305,174
b1248
b1249: cls - cardiolipin synthetase (NCBI), at 1,305,209 to 1,306,669
cls
Position (kb)
1303
1304
1305 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1302.812 kb on + strand at 1302.812 kb on + strand at 1302.860 kb on + strand at 1302.933 kb on + strand, within oppD at 1303.045 kb on + strand, within oppD at 1303.071 kb on + strand, within oppD at 1303.132 kb on + strand, within oppD at 1303.142 kb on + strand, within oppD at 1303.155 kb on + strand, within oppD at 1303.165 kb on + strand, within oppD at 1303.201 kb on - strand, within oppD at 1303.342 kb on - strand, within oppD at 1303.359 kb on + strand, within oppD at 1303.403 kb on - strand, within oppD at 1303.405 kb on + strand, within oppD at 1303.434 kb on - strand, within oppD at 1303.439 kb on + strand, within oppD at 1303.439 kb on + strand, within oppD at 1303.517 kb on + strand, within oppD at 1303.563 kb on + strand, within oppD at 1303.571 kb on - strand, within oppD at 1303.902 kb on + strand, within oppF at 1303.914 kb on - strand, within oppF at 1303.981 kb on + strand, within oppF at 1303.981 kb on + strand, within oppF at 1304.158 kb on + strand, within oppF at 1304.294 kb on + strand, within oppF at 1304.294 kb on + strand, within oppF at 1304.367 kb on + strand, within oppF at 1304.720 kb on + strand at 1304.780 kb on - strand at 1304.780 kb on - strand at 1305.078 kb on - strand, within b1248 at 1305.078 kb on - strand, within b1248 at 1305.176 kb on + strand at 1305.186 kb on + strand at 1305.539 kb on + strand, within cls at 1305.539 kb on + strand, within cls at 1305.576 kb on - strand, within cls at 1305.588 kb on - strand, within cls at 1305.588 kb on - strand, within cls at 1305.625 kb on - strand, within cls at 1305.641 kb on + strand, within cls at 1305.641 kb on + strand, within cls
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,302,812 + -0.0 1,302,812 + -0.4 1,302,860 + +0.6 1,302,933 + oppD b1246 0.15 -0.0 1,303,045 + oppD b1246 0.26 +0.4 1,303,071 + oppD b1246 0.29 -0.6 1,303,132 + oppD b1246 0.35 +0.3 1,303,142 + oppD b1246 0.36 +0.8 1,303,155 + oppD b1246 0.37 -0.8 1,303,165 + oppD b1246 0.38 +2.0 1,303,201 - oppD b1246 0.42 -1.8 1,303,342 - oppD b1246 0.56 -0.8 1,303,359 + oppD b1246 0.57 -0.6 1,303,403 - oppD b1246 0.62 -0.4 1,303,405 + oppD b1246 0.62 -1.8 1,303,434 - oppD b1246 0.65 -2.0 1,303,439 + oppD b1246 0.65 -0.4 1,303,439 + oppD b1246 0.65 +1.1 1,303,517 + oppD b1246 0.73 -0.1 1,303,563 + oppD b1246 0.77 -0.9 1,303,571 - oppD b1246 0.78 -1.3 1,303,902 + oppF b1247 0.11 -0.3 1,303,914 - oppF b1247 0.13 +0.1 1,303,981 + oppF b1247 0.19 +0.1 1,303,981 + oppF b1247 0.19 -0.8 1,304,158 + oppF b1247 0.37 -1.6 1,304,294 + oppF b1247 0.50 -2.5 1,304,294 + oppF b1247 0.50 -2.0 1,304,367 + oppF b1247 0.58 +0.6 1,304,720 + +1.2 1,304,780 - -0.4 1,304,780 - -1.4 1,305,078 - b1248 b1248 0.71 -0.1 1,305,078 - b1248 b1248 0.71 +0.3 1,305,176 + -2.1 1,305,186 + -0.3 1,305,539 + cls b1249 0.23 +0.7 1,305,539 + cls b1249 0.23 +1.1 1,305,576 - cls b1249 0.25 -0.5 1,305,588 - cls b1249 0.26 -0.6 1,305,588 - cls b1249 0.26 -1.8 1,305,625 - cls b1249 0.28 -0.7 1,305,641 + cls b1249 0.30 +0.7 1,305,641 + cls b1249 0.30 -0.0
Or see this region's nucleotide sequence