Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rssB and galU are separated by 201 nucleotides galU and hns are separated by 143 nucleotides
b1235: rssB - response regulator of RpoS (NCBI), at 1,289,465 to 1,290,478
rssB
b1236: galU - glucose-1-phosphate uridylyltransferase (NCBI), at 1,290,680 to 1,291,588
galU
b1237: hns - global DNA-binding transcriptional dual regulator H-NS (NCBI), at 1,291,732 to 1,292,145
hns
Position (kb)
1290
1291
1292 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1289.686 kb on + strand, within rssB at 1289.691 kb on - strand, within rssB at 1289.753 kb on - strand, within rssB at 1289.753 kb on - strand, within rssB at 1289.829 kb on + strand, within rssB at 1289.841 kb on - strand, within rssB at 1289.841 kb on - strand, within rssB at 1289.903 kb on + strand, within rssB at 1289.903 kb on + strand, within rssB at 1289.936 kb on + strand, within rssB at 1289.940 kb on + strand, within rssB at 1289.940 kb on + strand, within rssB at 1289.962 kb on + strand, within rssB at 1290.051 kb on - strand, within rssB at 1290.082 kb on - strand, within rssB at 1290.153 kb on + strand, within rssB at 1290.202 kb on - strand, within rssB at 1290.327 kb on - strand, within rssB at 1290.359 kb on - strand, within rssB at 1290.359 kb on - strand, within rssB at 1290.401 kb on + strand at 1290.426 kb on + strand at 1290.431 kb on + strand at 1290.538 kb on - strand at 1290.593 kb on + strand at 1290.594 kb on - strand at 1290.594 kb on - strand at 1290.602 kb on + strand at 1290.606 kb on + strand at 1290.609 kb on - strand at 1290.609 kb on - strand at 1290.612 kb on + strand at 1290.612 kb on + strand at 1290.612 kb on + strand at 1290.622 kb on + strand at 1290.664 kb on + strand at 1290.672 kb on - strand at 1290.672 kb on - strand at 1290.672 kb on - strand at 1290.672 kb on - strand at 1290.737 kb on + strand at 1290.755 kb on + strand at 1290.775 kb on - strand, within galU at 1290.785 kb on - strand, within galU at 1290.903 kb on + strand, within galU at 1290.903 kb on + strand, within galU at 1290.969 kb on + strand, within galU at 1291.001 kb on + strand, within galU at 1291.027 kb on - strand, within galU at 1291.030 kb on + strand, within galU at 1291.044 kb on - strand, within galU at 1291.047 kb on + strand, within galU at 1291.048 kb on + strand, within galU at 1291.048 kb on + strand, within galU at 1291.048 kb on + strand at 1291.131 kb on + strand, within galU at 1291.139 kb on + strand, within galU at 1291.157 kb on + strand, within galU at 1291.182 kb on + strand, within galU at 1291.182 kb on + strand, within galU at 1291.209 kb on + strand, within galU at 1291.209 kb on + strand, within galU at 1291.319 kb on + strand at 1291.375 kb on + strand, within galU at 1291.375 kb on + strand at 1291.375 kb on + strand, within galU at 1291.412 kb on + strand, within galU at 1291.413 kb on + strand, within galU at 1291.460 kb on + strand, within galU at 1291.558 kb on + strand at 1291.558 kb on + strand at 1291.565 kb on + strand at 1291.565 kb on + strand at 1291.605 kb on + strand at 1291.605 kb on + strand at 1291.607 kb on - strand at 1291.625 kb on - strand at 1291.642 kb on - strand at 1291.646 kb on + strand at 1291.886 kb on - strand, within hns at 1292.167 kb on - strand at 1292.218 kb on + strand at 1292.319 kb on - strand at 1292.353 kb on - strand at 1292.383 kb on + strand at 1292.394 kb on + strand at 1292.401 kb on + strand at 1292.409 kb on - strand at 1292.417 kb on - strand at 1292.419 kb on - strand at 1292.469 kb on - strand at 1292.469 kb on - strand at 1292.481 kb on + strand at 1292.497 kb on - strand at 1292.504 kb on + strand at 1292.504 kb on + strand at 1292.508 kb on + strand at 1292.512 kb on - strand at 1292.512 kb on - strand at 1292.519 kb on + strand at 1292.522 kb on - strand at 1292.522 kb on - strand at 1292.527 kb on - strand at 1292.527 kb on - strand at 1292.532 kb on - strand at 1292.537 kb on - strand at 1292.549 kb on - strand at 1292.549 kb on - strand at 1292.552 kb on + strand at 1292.552 kb on - strand at 1292.562 kb on + strand at 1292.572 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,289,686 + rssB b1235 0.22 -1.2 1,289,691 - rssB b1235 0.22 +1.0 1,289,753 - rssB b1235 0.28 +0.2 1,289,753 - rssB b1235 0.28 +0.2 1,289,829 + rssB b1235 0.36 -0.7 1,289,841 - rssB b1235 0.37 +0.2 1,289,841 - rssB b1235 0.37 -1.1 1,289,903 + rssB b1235 0.43 +0.3 1,289,903 + rssB b1235 0.43 -0.4 1,289,936 + rssB b1235 0.46 +0.3 1,289,940 + rssB b1235 0.47 +0.7 1,289,940 + rssB b1235 0.47 -1.6 1,289,962 + rssB b1235 0.49 +0.0 1,290,051 - rssB b1235 0.58 +0.6 1,290,082 - rssB b1235 0.61 -0.0 1,290,153 + rssB b1235 0.68 -0.3 1,290,202 - rssB b1235 0.73 +1.2 1,290,327 - rssB b1235 0.85 -2.5 1,290,359 - rssB b1235 0.88 +0.2 1,290,359 - rssB b1235 0.88 -0.0 1,290,401 + -0.9 1,290,426 + +1.6 1,290,431 + +0.2 1,290,538 - +1.6 1,290,593 + +1.0 1,290,594 - -0.8 1,290,594 - +2.2 1,290,602 + -0.0 1,290,606 + -0.6 1,290,609 - -1.4 1,290,609 - -1.8 1,290,612 + -0.9 1,290,612 + -0.6 1,290,612 + +0.1 1,290,622 + -0.2 1,290,664 + -0.2 1,290,672 - -0.4 1,290,672 - +2.2 1,290,672 - -0.0 1,290,672 - -0.2 1,290,737 + +0.6 1,290,755 + -0.9 1,290,775 - galU b1236 0.10 -0.8 1,290,785 - galU b1236 0.12 -0.4 1,290,903 + galU b1236 0.25 -0.8 1,290,903 + galU b1236 0.25 -1.4 1,290,969 + galU b1236 0.32 +0.8 1,291,001 + galU b1236 0.35 -0.4 1,291,027 - galU b1236 0.38 +2.6 1,291,030 + galU b1236 0.39 -1.2 1,291,044 - galU b1236 0.40 +1.6 1,291,047 + galU b1236 0.40 -0.4 1,291,048 + galU b1236 0.40 -0.8 1,291,048 + galU b1236 0.40 -0.4 1,291,048 + -0.0 1,291,131 + galU b1236 0.50 +1.6 1,291,139 + galU b1236 0.50 -1.6 1,291,157 + galU b1236 0.52 -1.6 1,291,182 + galU b1236 0.55 -0.0 1,291,182 + galU b1236 0.55 -1.9 1,291,209 + galU b1236 0.58 +0.6 1,291,209 + galU b1236 0.58 -0.8 1,291,319 + +1.6 1,291,375 + galU b1236 0.76 +0.6 1,291,375 + +0.6 1,291,375 + galU b1236 0.76 -1.6 1,291,412 + galU b1236 0.81 -0.9 1,291,413 + galU b1236 0.81 -0.4 1,291,460 + galU b1236 0.86 -0.4 1,291,558 + +0.4 1,291,558 + -0.2 1,291,565 + -0.4 1,291,565 + -0.3 1,291,605 + +0.4 1,291,605 + -0.4 1,291,607 - +1.6 1,291,625 - +1.6 1,291,642 - +0.5 1,291,646 + -0.0 1,291,886 - hns b1237 0.37 +0.6 1,292,167 - +0.6 1,292,218 + -0.0 1,292,319 - -1.5 1,292,353 - -0.2 1,292,383 + -0.0 1,292,394 + +0.1 1,292,401 + +1.6 1,292,409 - +2.1 1,292,417 - +0.6 1,292,419 - -0.0 1,292,469 - +1.0 1,292,469 - -1.0 1,292,481 + -0.0 1,292,497 - +0.8 1,292,504 + -0.2 1,292,504 + -1.0 1,292,508 + +2.0 1,292,512 - +1.6 1,292,512 - +0.8 1,292,519 + +1.6 1,292,522 - -1.6 1,292,522 - +0.2 1,292,527 - -0.0 1,292,527 - +0.1 1,292,532 - -0.8 1,292,537 - +1.6 1,292,549 - -1.2 1,292,549 - -1.2 1,292,552 + +0.3 1,292,552 - +0.2 1,292,562 + +0.6 1,292,572 + +1.6
Or see this region's nucleotide sequence