Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chaA and chaB are separated by 269 nucleotides chaB and chaC are separated by 157 nucleotides chaC and ychN are separated by 43 nucleotides
b1216: chaA - calcium/sodium:proton antiporter (NCBI), at 1,269,972 to 1,271,072
chaA
b1217: chaB - cation transport regulator (NCBI), at 1,271,342 to 1,271,572
chaB
b1218: chaC - regulatory protein for cation transport (RefSeq), at 1,271,730 to 1,272,425
chaC
b1219: ychN - hypothetical protein (NCBI), at 1,272,469 to 1,272,822
ychN
Position (kb)
1271
1272 Strain fitness (log2 ratio)
-1
0
1 at 1270.415 kb on - strand, within chaA at 1270.552 kb on + strand, within chaA at 1270.588 kb on + strand, within chaA at 1270.615 kb on + strand, within chaA at 1270.622 kb on - strand, within chaA at 1270.637 kb on + strand, within chaA at 1270.639 kb on + strand, within chaA at 1270.704 kb on - strand, within chaA at 1270.704 kb on - strand, within chaA at 1270.750 kb on - strand, within chaA at 1270.755 kb on - strand, within chaA at 1270.780 kb on - strand, within chaA at 1270.790 kb on - strand, within chaA at 1270.790 kb on - strand, within chaA at 1270.878 kb on + strand, within chaA at 1270.878 kb on + strand, within chaA at 1270.972 kb on - strand at 1270.972 kb on - strand at 1270.977 kb on - strand at 1270.986 kb on + strand at 1270.988 kb on + strand at 1270.994 kb on + strand at 1270.994 kb on + strand at 1271.045 kb on - strand at 1271.054 kb on + strand at 1271.074 kb on + strand at 1271.074 kb on + strand at 1271.083 kb on - strand at 1271.083 kb on - strand at 1271.145 kb on + strand at 1271.248 kb on + strand at 1271.254 kb on - strand at 1271.256 kb on - strand at 1271.342 kb on - strand at 1271.347 kb on + strand at 1271.381 kb on + strand, within chaB at 1271.381 kb on + strand, within chaB at 1271.475 kb on - strand, within chaB at 1271.723 kb on + strand at 1271.892 kb on + strand, within chaC at 1271.892 kb on + strand, within chaC at 1271.932 kb on - strand, within chaC at 1272.143 kb on - strand, within chaC at 1272.159 kb on - strand, within chaC at 1272.288 kb on - strand, within chaC at 1272.522 kb on - strand, within ychN at 1272.532 kb on - strand, within ychN
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,270,415 - chaA b1216 0.40 -0.2 1,270,552 + chaA b1216 0.53 -0.1 1,270,588 + chaA b1216 0.56 -0.2 1,270,615 + chaA b1216 0.58 -0.5 1,270,622 - chaA b1216 0.59 +0.4 1,270,637 + chaA b1216 0.60 +0.2 1,270,639 + chaA b1216 0.61 -0.8 1,270,704 - chaA b1216 0.66 +0.3 1,270,704 - chaA b1216 0.66 -0.2 1,270,750 - chaA b1216 0.71 -0.6 1,270,755 - chaA b1216 0.71 -1.1 1,270,780 - chaA b1216 0.73 +0.5 1,270,790 - chaA b1216 0.74 -0.2 1,270,790 - chaA b1216 0.74 +0.3 1,270,878 + chaA b1216 0.82 +0.4 1,270,878 + chaA b1216 0.82 +0.6 1,270,972 - -0.6 1,270,972 - -1.7 1,270,977 - -0.6 1,270,986 + -0.3 1,270,988 + +0.3 1,270,994 + +0.4 1,270,994 + +0.4 1,271,045 - +0.1 1,271,054 + -0.0 1,271,074 + -0.1 1,271,074 + -0.3 1,271,083 - +1.6 1,271,083 - -1.2 1,271,145 + -0.2 1,271,248 + +0.1 1,271,254 - -0.7 1,271,256 - -0.8 1,271,342 - -0.0 1,271,347 + +0.8 1,271,381 + chaB b1217 0.17 -0.7 1,271,381 + chaB b1217 0.17 -0.0 1,271,475 - chaB b1217 0.58 +1.3 1,271,723 + +1.1 1,271,892 + chaC b1218 0.23 +1.1 1,271,892 + chaC b1218 0.23 -0.1 1,271,932 - chaC b1218 0.29 -0.4 1,272,143 - chaC b1218 0.59 -0.0 1,272,159 - chaC b1218 0.62 -0.2 1,272,288 - chaC b1218 0.80 +0.5 1,272,522 - ychN b1219 0.15 +0.1 1,272,532 - ychN b1219 0.18 -0.2
Or see this region's nucleotide sequence