Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycfZ and ymfA overlap by 4 nucleotides ymfA and potD are separated by 57 nucleotides potD and potC overlap by 4 nucleotides potC and potB overlap by 4 nucleotides
b1121: ycfZ - predicted inner membrane protein (NCBI), at 1,179,702 to 1,180,490
ycfZ
b1122: ymfA - orf, hypothetical protein (VIMSS), at 1,180,487 to 1,180,948
ymfA
b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052
potD
b1124: potC - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,049 to 1,182,843
potC
b1125: potB - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,840 to 1,183,667
potB
Position (kb)
1181
1182
1183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1180.234 kb on - strand, within ycfZ at 1180.389 kb on - strand, within ycfZ at 1180.389 kb on - strand, within ycfZ at 1180.438 kb on + strand at 1180.457 kb on + strand at 1180.739 kb on - strand, within ymfA at 1180.755 kb on - strand, within ymfA at 1180.755 kb on - strand, within ymfA at 1180.763 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.778 kb on - strand, within ymfA at 1180.796 kb on + strand, within ymfA at 1180.796 kb on + strand, within ymfA at 1180.799 kb on - strand, within ymfA at 1180.800 kb on - strand, within ymfA at 1180.803 kb on - strand, within ymfA at 1180.809 kb on + strand, within ymfA at 1180.813 kb on + strand, within ymfA at 1180.817 kb on - strand, within ymfA at 1180.834 kb on - strand, within ymfA at 1180.838 kb on - strand, within ymfA at 1180.854 kb on - strand, within ymfA at 1180.869 kb on - strand, within ymfA at 1180.881 kb on + strand, within ymfA at 1180.881 kb on + strand, within ymfA at 1180.890 kb on + strand, within ymfA at 1180.894 kb on + strand, within ymfA at 1180.894 kb on + strand, within ymfA at 1180.894 kb on + strand, within ymfA at 1180.900 kb on + strand, within ymfA at 1180.900 kb on + strand, within ymfA at 1180.902 kb on - strand at 1180.908 kb on - strand at 1180.937 kb on + strand at 1180.945 kb on - strand at 1180.945 kb on - strand at 1180.953 kb on - strand at 1180.959 kb on - strand at 1180.988 kb on - strand at 1180.988 kb on - strand at 1180.989 kb on - strand at 1180.989 kb on - strand at 1181.036 kb on - strand at 1181.046 kb on - strand at 1181.101 kb on + strand at 1181.101 kb on + strand at 1181.170 kb on - strand, within potD at 1181.280 kb on - strand, within potD at 1181.280 kb on - strand, within potD at 1181.421 kb on + strand, within potD at 1181.480 kb on - strand, within potD at 1181.522 kb on - strand, within potD at 1181.539 kb on + strand, within potD at 1181.547 kb on - strand, within potD at 1181.547 kb on - strand, within potD at 1181.556 kb on + strand, within potD at 1181.556 kb on + strand, within potD at 1181.581 kb on - strand, within potD at 1181.684 kb on - strand, within potD at 1181.721 kb on - strand, within potD at 1181.839 kb on - strand, within potD at 1181.865 kb on - strand, within potD at 1181.918 kb on + strand, within potD at 1182.003 kb on + strand at 1182.395 kb on + strand, within potC at 1182.474 kb on - strand, within potC at 1182.476 kb on - strand, within potC at 1182.524 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.589 kb on + strand, within potC at 1182.668 kb on + strand, within potC at 1182.691 kb on + strand, within potC at 1182.700 kb on + strand, within potC at 1182.730 kb on + strand, within potC at 1182.910 kb on + strand at 1182.910 kb on + strand at 1182.914 kb on - strand at 1183.032 kb on + strand, within potB at 1183.032 kb on + strand, within potB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,180,234 - ycfZ b1121 0.67 +1.6 1,180,389 - ycfZ b1121 0.87 +1.6 1,180,389 - ycfZ b1121 0.87 +1.6 1,180,438 + +1.6 1,180,457 + +1.6 1,180,739 - ymfA b1122 0.55 -0.0 1,180,755 - ymfA b1122 0.58 -2.1 1,180,755 - ymfA b1122 0.58 +0.3 1,180,763 - ymfA b1122 0.60 +1.6 1,180,777 - ymfA b1122 0.63 -0.8 1,180,777 - ymfA b1122 0.63 +2.2 1,180,777 - ymfA b1122 0.63 +0.6 1,180,777 - ymfA b1122 0.63 -0.8 1,180,777 - ymfA b1122 0.63 -0.4 1,180,777 - ymfA b1122 0.63 -0.0 1,180,778 - ymfA b1122 0.63 -0.5 1,180,796 + ymfA b1122 0.67 +0.6 1,180,796 + ymfA b1122 0.67 +0.6 1,180,799 - ymfA b1122 0.68 +0.0 1,180,800 - ymfA b1122 0.68 +0.0 1,180,803 - ymfA b1122 0.68 +0.1 1,180,809 + ymfA b1122 0.70 +0.6 1,180,813 + ymfA b1122 0.71 -0.4 1,180,817 - ymfA b1122 0.71 +0.4 1,180,834 - ymfA b1122 0.75 -0.4 1,180,838 - ymfA b1122 0.76 +0.6 1,180,854 - ymfA b1122 0.79 -0.4 1,180,869 - ymfA b1122 0.83 +0.0 1,180,881 + ymfA b1122 0.85 +0.7 1,180,881 + ymfA b1122 0.85 +0.4 1,180,890 + ymfA b1122 0.87 -0.7 1,180,894 + ymfA b1122 0.88 -0.3 1,180,894 + ymfA b1122 0.88 -0.3 1,180,894 + ymfA b1122 0.88 -0.2 1,180,900 + ymfA b1122 0.89 -0.1 1,180,900 + ymfA b1122 0.89 -0.9 1,180,902 - -0.4 1,180,908 - +0.0 1,180,937 + -0.7 1,180,945 - +0.6 1,180,945 - +0.6 1,180,953 - +0.6 1,180,959 - -0.5 1,180,988 - +1.6 1,180,988 - +1.0 1,180,989 - -0.7 1,180,989 - -1.4 1,181,036 - -0.7 1,181,046 - -2.2 1,181,101 + +1.6 1,181,101 + +1.6 1,181,170 - potD b1123 0.16 +0.6 1,181,280 - potD b1123 0.26 -0.4 1,181,280 - potD b1123 0.26 -0.6 1,181,421 + potD b1123 0.40 -1.1 1,181,480 - potD b1123 0.45 +0.6 1,181,522 - potD b1123 0.49 +0.9 1,181,539 + potD b1123 0.51 -0.1 1,181,547 - potD b1123 0.52 -0.7 1,181,547 - potD b1123 0.52 -1.8 1,181,556 + potD b1123 0.53 +0.6 1,181,556 + potD b1123 0.53 -0.5 1,181,581 - potD b1123 0.55 +0.0 1,181,684 - potD b1123 0.65 -1.2 1,181,721 - potD b1123 0.68 -0.7 1,181,839 - potD b1123 0.80 +0.2 1,181,865 - potD b1123 0.82 -1.4 1,181,918 + potD b1123 0.87 -1.1 1,182,003 + +0.5 1,182,395 + potC b1124 0.44 -0.7 1,182,474 - potC b1124 0.53 -0.3 1,182,476 - potC b1124 0.54 -0.7 1,182,524 + potC b1124 0.60 -0.7 1,182,581 + potC b1124 0.67 +0.2 1,182,581 + potC b1124 0.67 -0.4 1,182,589 + potC b1124 0.68 +0.2 1,182,668 + potC b1124 0.78 -0.6 1,182,691 + potC b1124 0.81 -0.4 1,182,700 + potC b1124 0.82 -1.3 1,182,730 + potC b1124 0.86 -0.1 1,182,910 + -1.1 1,182,910 + -0.6 1,182,914 - -0.6 1,183,032 + potB b1125 0.23 -0.9 1,183,032 + potB b1125 0.23 +0.4
Or see this region's nucleotide sequence