Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycfF and ycfL are separated by 2 nucleotides ycfL and ycfM are separated by 13 nucleotides ycfM and ycfN overlap by 20 nucleotides ycfN and nagZ are separated by 10 nucleotides
b1103: ycfF - orf, hypothetical protein (VIMSS), at 1,161,108 to 1,161,467
ycfF
b1104: ycfL - hypothetical protein (NCBI), at 1,161,470 to 1,161,847
ycfL
b1105: ycfM - predicted outer membrane lipoprotein (NCBI), at 1,161,861 to 1,162,502
ycfM
b1106: ycfN - thiamin kinase (NCBI), at 1,162,483 to 1,163,307
ycfN
b1107: nagZ - beta-hexosaminidase (NCBI), at 1,163,318 to 1,164,343
nagZ
Position (kb)
1161
1162
1163 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1161.023 kb on + strand at 1161.023 kb on + strand at 1161.034 kb on + strand at 1161.048 kb on - strand at 1161.048 kb on - strand at 1161.180 kb on - strand, within ycfF at 1161.414 kb on - strand, within ycfF at 1161.508 kb on - strand, within ycfL at 1161.648 kb on - strand, within ycfL at 1161.659 kb on + strand, within ycfL at 1161.996 kb on + strand, within ycfM at 1162.014 kb on + strand, within ycfM at 1162.014 kb on + strand, within ycfM at 1162.190 kb on - strand, within ycfM at 1162.347 kb on - strand, within ycfM at 1162.456 kb on - strand at 1162.640 kb on + strand, within ycfN at 1162.873 kb on - strand, within ycfN at 1162.875 kb on + strand, within ycfN at 1162.992 kb on - strand, within ycfN at 1163.024 kb on + strand, within ycfN at 1163.057 kb on - strand, within ycfN at 1163.213 kb on - strand, within ycfN at 1163.219 kb on + strand, within ycfN at 1163.220 kb on + strand, within ycfN at 1163.313 kb on + strand at 1163.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,161,023 + +0.6 1,161,023 + +2.1 1,161,034 + -0.2 1,161,048 - +0.3 1,161,048 - +0.2 1,161,180 - ycfF b1103 0.20 +1.2 1,161,414 - ycfF b1103 0.85 +0.3 1,161,508 - ycfL b1104 0.10 -0.2 1,161,648 - ycfL b1104 0.47 +0.1 1,161,659 + ycfL b1104 0.50 +0.1 1,161,996 + ycfM b1105 0.21 -0.6 1,162,014 + ycfM b1105 0.24 -2.5 1,162,014 + ycfM b1105 0.24 -0.9 1,162,190 - ycfM b1105 0.51 -0.9 1,162,347 - ycfM b1105 0.76 -1.0 1,162,456 - -0.6 1,162,640 + ycfN b1106 0.19 -1.5 1,162,873 - ycfN b1106 0.47 -0.2 1,162,875 + ycfN b1106 0.48 +0.8 1,162,992 - ycfN b1106 0.62 +0.2 1,163,024 + ycfN b1106 0.66 -0.0 1,163,057 - ycfN b1106 0.70 -0.6 1,163,213 - ycfN b1106 0.88 -0.2 1,163,219 + ycfN b1106 0.89 +0.6 1,163,220 + ycfN b1106 0.89 +0.6 1,163,313 + +0.5 1,163,324 - +1.0
Or see this region's nucleotide sequence