Strain Fitness in Escherichia coli BW25113 around b3153

Experiment: LB Anaerobic with Azlocillin 0.00003125 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntyraQ and yraR are separated by 72 nucleotidesyraR and yhbO are separated by 127 nucleotidesyhbO and yhbP overlap by 21 nucleotidesyhbP and yhbQ are separated by 50 nucleotidesyhbQ and yhbS overlap by 14 nucleotides b3151: yraQ - predicted permease (NCBI), at 3,295,120 to 3,296,160 yraQ b3152: yraR - orf, hypothetical protein (VIMSS), at 3,296,233 to 3,296,868 yraR b3153: yhbO - orf, hypothetical protein (VIMSS), at 3,296,996 to 3,297,514 yhbO b3154: yhbP - hypothetical protein (NCBI), at 3,297,494 to 3,297,937 yhbP b3155: yhbQ - hypothetical protein (NCBI), at 3,297,988 to 3,298,290 yhbQ b3156: yhbS - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 3,298,277 to 3,298,780 yhbS Position (kb) 3296 3297 3298Strain fitness (log2 ratio) -3 -2 -1 0 1at 3296.007 kb on + strand, within yraQat 3296.053 kb on - strand, within yraQat 3296.053 kb on - strand, within yraQat 3296.059 kb on - strandat 3296.059 kb on - strandat 3296.196 kb on + strandat 3296.201 kb on + strandat 3296.201 kb on + strandat 3296.221 kb on - strandat 3296.221 kb on - strandat 3296.289 kb on - strandat 3296.289 kb on - strandat 3296.294 kb on - strandat 3296.441 kb on - strand, within yraRat 3296.441 kb on - strand, within yraRat 3296.466 kb on - strand, within yraRat 3296.549 kb on + strand, within yraRat 3296.661 kb on + strand, within yraRat 3296.661 kb on + strand, within yraRat 3296.687 kb on - strand, within yraRat 3296.737 kb on - strand, within yraRat 3296.737 kb on - strand, within yraRat 3296.819 kb on + strandat 3296.819 kb on + strandat 3296.830 kb on + strandat 3296.927 kb on - strandat 3296.992 kb on + strandat 3296.992 kb on + strandat 3297.032 kb on - strandat 3297.149 kb on - strand, within yhbOat 3297.149 kb on - strand, within yhbOat 3297.295 kb on - strand, within yhbOat 3297.383 kb on - strand, within yhbOat 3297.383 kb on - strand, within yhbOat 3297.383 kb on - strand, within yhbOat 3297.395 kb on - strand, within yhbOat 3297.395 kb on - strand, within yhbOat 3297.432 kb on + strand, within yhbOat 3297.491 kb on + strandat 3297.491 kb on + strandat 3297.515 kb on - strandat 3297.553 kb on - strand, within yhbPat 3297.632 kb on + strand, within yhbPat 3297.632 kb on + strand, within yhbPat 3297.639 kb on + strand, within yhbPat 3297.667 kb on + strand, within yhbPat 3297.740 kb on - strand, within yhbPat 3297.740 kb on - strand, within yhbPat 3297.770 kb on + strand, within yhbPat 3297.842 kb on + strand, within yhbPat 3297.852 kb on + strand, within yhbPat 3297.857 kb on + strand, within yhbPat 3297.879 kb on + strand, within yhbPat 3297.889 kb on - strand, within yhbPat 3297.889 kb on - strand, within yhbPat 3297.960 kb on + strandat 3297.967 kb on + strandat 3297.967 kb on + strandat 3297.974 kb on - strandat 3298.077 kb on + strand, within yhbQat 3298.077 kb on + strand, within yhbQat 3298.121 kb on + strand, within yhbQat 3298.129 kb on + strand, within yhbQat 3298.129 kb on + strand, within yhbQat 3298.238 kb on + strand, within yhbQat 3298.238 kb on + strand, within yhbQat 3298.249 kb on + strand, within yhbQat 3298.298 kb on + strandat 3298.298 kb on + strandat 3298.304 kb on - strandat 3298.339 kb on + strand, within yhbSat 3298.411 kb on + strand, within yhbSat 3298.411 kb on + strand, within yhbSat 3298.427 kb on + strand, within yhbSat 3298.435 kb on - strand, within yhbSat 3298.459 kb on - strand, within yhbSat 3298.483 kb on - strand, within yhbSat 3298.485 kb on - strand, within yhbSat 3298.495 kb on - strand, within yhbSat 3298.497 kb on + strand, within yhbSat 3298.504 kb on - strand, within yhbS

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Azlocillin 0.00003125 mM
remove
3,296,007 + yraQ b3151 0.85 -0.5
3,296,053 - yraQ b3151 0.90 +0.1
3,296,053 - yraQ b3151 0.90 +1.2
3,296,059 - -0.7
3,296,059 - -0.3
3,296,196 + +0.1
3,296,201 + +0.3
3,296,201 + +0.3
3,296,221 - -2.0
3,296,221 - -0.2
3,296,289 - -1.2
3,296,289 - -3.1
3,296,294 - -0.1
3,296,441 - yraR b3152 0.33 +0.2
3,296,441 - yraR b3152 0.33 -0.7
3,296,466 - yraR b3152 0.37 -0.5
3,296,549 + yraR b3152 0.50 +0.5
3,296,661 + yraR b3152 0.67 +0.4
3,296,661 + yraR b3152 0.67 +0.4
3,296,687 - yraR b3152 0.71 -0.1
3,296,737 - yraR b3152 0.79 +1.4
3,296,737 - yraR b3152 0.79 -0.6
3,296,819 + +0.8
3,296,819 + +0.6
3,296,830 + -0.3
3,296,927 - -0.0
3,296,992 + -0.9
3,296,992 + +0.1
3,297,032 - +0.2
3,297,149 - yhbO b3153 0.29 -1.3
3,297,149 - yhbO b3153 0.29 +0.3
3,297,295 - yhbO b3153 0.58 -1.1
3,297,383 - yhbO b3153 0.75 -2.2
3,297,383 - yhbO b3153 0.75 -0.9
3,297,383 - yhbO b3153 0.75 -0.7
3,297,395 - yhbO b3153 0.77 +0.2
3,297,395 - yhbO b3153 0.77 -0.4
3,297,432 + yhbO b3153 0.84 -0.6
3,297,491 + +0.7
3,297,491 + -0.0
3,297,515 - -0.3
3,297,553 - yhbP b3154 0.13 -0.2
3,297,632 + yhbP b3154 0.31 +0.6
3,297,632 + yhbP b3154 0.31 -0.9
3,297,639 + yhbP b3154 0.33 -0.4
3,297,667 + yhbP b3154 0.39 -0.8
3,297,740 - yhbP b3154 0.55 +0.4
3,297,740 - yhbP b3154 0.55 -0.2
3,297,770 + yhbP b3154 0.62 -1.5
3,297,842 + yhbP b3154 0.78 -0.3
3,297,852 + yhbP b3154 0.81 -0.1
3,297,857 + yhbP b3154 0.82 +0.4
3,297,879 + yhbP b3154 0.87 -0.0
3,297,889 - yhbP b3154 0.89 +1.3
3,297,889 - yhbP b3154 0.89 -0.1
3,297,960 + -1.0
3,297,967 + -0.2
3,297,967 + +0.6
3,297,974 - -0.8
3,298,077 + yhbQ b3155 0.29 -0.1
3,298,077 + yhbQ b3155 0.29 -0.7
3,298,121 + yhbQ b3155 0.44 +0.1
3,298,129 + yhbQ b3155 0.47 -0.3
3,298,129 + yhbQ b3155 0.47 +0.1
3,298,238 + yhbQ b3155 0.83 -0.1
3,298,238 + yhbQ b3155 0.83 -0.6
3,298,249 + yhbQ b3155 0.86 +0.3
3,298,298 + +1.0
3,298,298 + +0.9
3,298,304 - +0.4
3,298,339 + yhbS b3156 0.12 +0.0
3,298,411 + yhbS b3156 0.27 -0.2
3,298,411 + yhbS b3156 0.27 +1.3
3,298,427 + yhbS b3156 0.30 -0.1
3,298,435 - yhbS b3156 0.31 +0.6
3,298,459 - yhbS b3156 0.36 -0.4
3,298,483 - yhbS b3156 0.41 +0.3
3,298,485 - yhbS b3156 0.41 +0.3
3,298,495 - yhbS b3156 0.43 -0.8
3,298,497 + yhbS b3156 0.44 +0.2
3,298,504 - yhbS b3156 0.45 +0.2

Or see this region's nucleotide sequence