Experiment: LB Anaerobic with Oxytetracycline 0.00000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fnr and ogt are separated by 194 nucleotides ogt and ydaH are separated by 10 nucleotides ydaH and abgB are separated by 36 nucleotides
b1334: fnr - DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI), at 1,396,798 to 1,397,550
fnr
b1335: ogt - O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI), at 1,397,745 to 1,398,260
ogt
b1336: ydaH - putative pump protein (transport) (VIMSS), at 1,398,271 to 1,399,797
ydaH
b1337: abgB - predicted peptidase, aminobenzoyl-glutamate utilization protein (NCBI), at 1,399,834 to 1,401,279
abgB
Position (kb)
1398
1399
1400 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1397.287 kb on - strand, within fnr at 1397.605 kb on + strand at 1397.605 kb on + strand at 1397.857 kb on - strand, within ogt at 1397.857 kb on - strand, within ogt at 1397.903 kb on + strand, within ogt at 1397.903 kb on + strand, within ogt at 1398.130 kb on + strand, within ogt at 1398.130 kb on + strand, within ogt at 1398.138 kb on + strand, within ogt at 1398.138 kb on + strand, within ogt at 1398.666 kb on + strand, within ydaH at 1398.666 kb on + strand, within ydaH at 1398.781 kb on - strand, within ydaH at 1398.822 kb on - strand, within ydaH at 1398.822 kb on - strand, within ydaH at 1398.846 kb on - strand, within ydaH at 1398.968 kb on - strand, within ydaH at 1398.968 kb on - strand, within ydaH at 1399.281 kb on - strand, within ydaH at 1399.573 kb on + strand, within ydaH at 1399.678 kb on + strand at 1399.678 kb on + strand at 1399.747 kb on + strand at 1399.795 kb on + strand at 1399.937 kb on + strand at 1400.259 kb on + strand, within abgB at 1400.259 kb on + strand, within abgB at 1400.627 kb on + strand, within abgB at 1400.627 kb on + strand, within abgB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Oxytetracycline 0.00000015625 mM remove 1,397,287 - fnr b1334 0.65 -1.0 1,397,605 + -0.6 1,397,605 + +0.2 1,397,857 - ogt b1335 0.22 -0.5 1,397,857 - ogt b1335 0.22 +0.2 1,397,903 + ogt b1335 0.31 -0.7 1,397,903 + ogt b1335 0.31 -0.8 1,398,130 + ogt b1335 0.75 +0.1 1,398,130 + ogt b1335 0.75 +0.5 1,398,138 + ogt b1335 0.76 +0.8 1,398,138 + ogt b1335 0.76 -1.2 1,398,666 + ydaH b1336 0.26 -0.7 1,398,666 + ydaH b1336 0.26 +0.7 1,398,781 - ydaH b1336 0.33 -0.5 1,398,822 - ydaH b1336 0.36 +2.1 1,398,822 - ydaH b1336 0.36 +0.2 1,398,846 - ydaH b1336 0.38 -0.2 1,398,968 - ydaH b1336 0.46 +1.3 1,398,968 - ydaH b1336 0.46 -1.8 1,399,281 - ydaH b1336 0.66 +1.0 1,399,573 + ydaH b1336 0.85 +0.7 1,399,678 + +0.7 1,399,678 + -0.0 1,399,747 + +0.5 1,399,795 + +0.0 1,399,937 + +0.3 1,400,259 + abgB b1337 0.29 +0.5 1,400,259 + abgB b1337 0.29 -0.5 1,400,627 + abgB b1337 0.55 -0.1 1,400,627 + abgB b1337 0.55 +0.6
Or see this region's nucleotide sequence