Experiment: 1,4-Butanediol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4538 and Atu4539 are separated by 12 nucleotides Atu4539 and Atu4540 are separated by 2 nucleotides Atu4540 and Atu4541 overlap by 4 nucleotides Atu4541 and Atu4542 are separated by 3 nucleotides
Atu4538: Atu4538 - 3-carboxy-cis,cis-muconate cycloisomerase, at 1,684,759 to 1,685,820
Atu4538
Atu4539: Atu4539 - protocatechuate 3,4-dioxygenase alpha chain, at 1,685,833 to 1,686,453
Atu4539
Atu4540: Atu4540 - protocatechuate 3,4-dioxygenase subunit beta, at 1,686,456 to 1,687,196
Atu4540
Atu4541: Atu4541 - carboxymuconolactone decarboxylase, at 1,687,193 to 1,687,627
Atu4541
Atu4542: Atu4542 - beta-ketoadipate enol-lactone hydrolase, at 1,687,631 to 1,688,428
Atu4542
Position (kb)
1686
1687
1688 Strain fitness (log2 ratio)
-2
-1
0
1 at 1685.601 kb on + strand, within Atu4538 at 1685.687 kb on + strand, within Atu4538 at 1685.689 kb on - strand, within Atu4538 at 1685.727 kb on - strand at 1685.744 kb on + strand at 1685.752 kb on - strand at 1685.760 kb on - strand at 1685.781 kb on + strand at 1685.789 kb on - strand at 1685.893 kb on - strand at 1685.912 kb on + strand, within Atu4539 at 1685.958 kb on - strand, within Atu4539 at 1685.984 kb on + strand, within Atu4539 at 1686.012 kb on - strand, within Atu4539 at 1686.014 kb on - strand, within Atu4539 at 1686.055 kb on - strand, within Atu4539 at 1686.092 kb on - strand, within Atu4539 at 1686.238 kb on - strand, within Atu4539 at 1686.247 kb on + strand, within Atu4539 at 1686.268 kb on + strand, within Atu4539 at 1686.268 kb on + strand, within Atu4539 at 1686.268 kb on + strand, within Atu4539 at 1686.268 kb on + strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.276 kb on - strand, within Atu4539 at 1686.452 kb on - strand at 1686.485 kb on + strand at 1686.511 kb on + strand at 1686.543 kb on - strand, within Atu4540 at 1686.543 kb on - strand, within Atu4540 at 1686.558 kb on - strand, within Atu4540 at 1686.630 kb on - strand, within Atu4540 at 1687.003 kb on - strand, within Atu4540 at 1687.134 kb on - strand at 1687.311 kb on + strand, within Atu4541 at 1687.319 kb on + strand, within Atu4541 at 1687.327 kb on - strand, within Atu4541 at 1687.356 kb on + strand, within Atu4541 at 1687.377 kb on + strand, within Atu4541 at 1687.385 kb on - strand, within Atu4541 at 1687.559 kb on + strand, within Atu4541 at 1687.581 kb on + strand, within Atu4541 at 1687.581 kb on + strand, within Atu4541 at 1687.669 kb on + strand at 1687.687 kb on - strand at 1687.690 kb on + strand at 1687.690 kb on + strand at 1687.715 kb on + strand, within Atu4542 at 1687.780 kb on + strand, within Atu4542 at 1687.830 kb on + strand, within Atu4542 at 1687.848 kb on - strand, within Atu4542 at 1687.948 kb on + strand, within Atu4542 at 1687.956 kb on - strand, within Atu4542 at 1687.956 kb on - strand, within Atu4542 at 1687.956 kb on - strand, within Atu4542 at 1687.991 kb on - strand, within Atu4542 at 1688.039 kb on + strand, within Atu4542 at 1688.094 kb on + strand, within Atu4542
Per-strain Table
Position Strand Gene LocusTag Fraction 1,4-Butanediol (C) remove 1,685,601 + Atu4538 0.79 -0.2 1,685,687 + Atu4538 0.87 -0.1 1,685,689 - Atu4538 0.88 -0.3 1,685,727 - -0.9 1,685,744 + -0.0 1,685,752 - -1.0 1,685,760 - -0.5 1,685,781 + +0.1 1,685,789 - -0.6 1,685,893 - +0.0 1,685,912 + Atu4539 0.13 +0.1 1,685,958 - Atu4539 0.20 +0.5 1,685,984 + Atu4539 0.24 +0.4 1,686,012 - Atu4539 0.29 +0.3 1,686,014 - Atu4539 0.29 -1.7 1,686,055 - Atu4539 0.36 -0.2 1,686,092 - Atu4539 0.42 -0.2 1,686,238 - Atu4539 0.65 -0.4 1,686,247 + Atu4539 0.67 +0.5 1,686,268 + Atu4539 0.70 +0.1 1,686,268 + Atu4539 0.70 -1.4 1,686,268 + Atu4539 0.70 -1.5 1,686,268 + Atu4539 0.70 +0.3 1,686,276 - Atu4539 0.71 +0.1 1,686,276 - Atu4539 0.71 +0.3 1,686,276 - Atu4539 0.71 -1.5 1,686,276 - Atu4539 0.71 +0.4 1,686,276 - Atu4539 0.71 +0.4 1,686,452 - +0.5 1,686,485 + +0.1 1,686,511 + -0.2 1,686,543 - Atu4540 0.12 +0.9 1,686,543 - Atu4540 0.12 +0.4 1,686,558 - Atu4540 0.14 +0.0 1,686,630 - Atu4540 0.23 +0.5 1,687,003 - Atu4540 0.74 -0.2 1,687,134 - +0.3 1,687,311 + Atu4541 0.27 -2.5 1,687,319 + Atu4541 0.29 -0.8 1,687,327 - Atu4541 0.31 -1.0 1,687,356 + Atu4541 0.37 -1.0 1,687,377 + Atu4541 0.42 -2.4 1,687,385 - Atu4541 0.44 +0.1 1,687,559 + Atu4541 0.84 -0.4 1,687,581 + Atu4541 0.89 -1.2 1,687,581 + Atu4541 0.89 -0.6 1,687,669 + -0.7 1,687,687 - -0.2 1,687,690 + +0.4 1,687,690 + +0.0 1,687,715 + Atu4542 0.11 -0.2 1,687,780 + Atu4542 0.19 +0.4 1,687,830 + Atu4542 0.25 -0.5 1,687,848 - Atu4542 0.27 -0.9 1,687,948 + Atu4542 0.40 -0.2 1,687,956 - Atu4542 0.41 -0.1 1,687,956 - Atu4542 0.41 +0.2 1,687,956 - Atu4542 0.41 +0.7 1,687,991 - Atu4542 0.45 +0.2 1,688,039 + Atu4542 0.51 +0.0 1,688,094 + Atu4542 0.58 +0.1
Or see this region's nucleotide sequence