Strain Fitness in Escherichia coli BW25113 around b3761

Experiment: LB Anaerobic with Spiramycin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrrlC and rrfC are separated by 92 nucleotidesrrfC and aspT are separated by 52 nucleotidesaspT and trpT are separated by 8 nucleotidestrpT and hdfR are separated by 95 nucleotides b3758: rrlC - 23S ribosomal RNA (NCBI), at 3,941,727 to 3,944,630 rrlC b3759: rrfC - 5S ribosomal RNA (NCBI), at 3,944,723 to 3,944,842 rrfC b3760: aspT - tRNA-Asp (NCBI), at 3,944,895 to 3,944,971 aspT b3761: trpT - tRNA-Trp (NCBI), at 3,944,980 to 3,945,055 trpT b4480: hdfR - transcriptional regulator HdfR (NCBI), at 3,945,151 to 3,945,990 hdfR Position (kb) 3944 3945 3946Strain fitness (log2 ratio) -2 -1 0 1at 3944.961 kb on + strand, within aspTat 3944.971 kb on + strandat 3945.046 kb on + strand, within trpTat 3945.298 kb on + strand, within hdfRat 3945.298 kb on + strand, within hdfRat 3945.322 kb on - strand, within hdfRat 3945.322 kb on - strand, within hdfRat 3945.390 kb on - strand, within hdfRat 3945.392 kb on + strand, within hdfRat 3945.392 kb on + strand, within hdfRat 3945.402 kb on - strand, within hdfRat 3945.402 kb on - strand, within hdfRat 3945.543 kb on + strand, within hdfRat 3945.543 kb on + strand, within hdfRat 3945.551 kb on - strand, within hdfRat 3945.744 kb on - strand, within hdfRat 3945.783 kb on + strand, within hdfRat 3945.783 kb on + strand, within hdfRat 3945.897 kb on + strand, within hdfRat 3945.902 kb on + strand, within hdfRat 3945.914 kb on - strandat 3946.030 kb on - strandat 3946.030 kb on - strandat 3946.045 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM
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3,944,961 + aspT b3760 0.86 +0.1
3,944,971 + -0.4
3,945,046 + trpT b3761 0.87 -1.3
3,945,298 + hdfR b4480 0.17 +0.5
3,945,298 + hdfR b4480 0.17 +1.0
3,945,322 - hdfR b4480 0.20 -1.3
3,945,322 - hdfR b4480 0.20 -0.8
3,945,390 - hdfR b4480 0.28 -1.9
3,945,392 + hdfR b4480 0.29 -0.5
3,945,392 + hdfR b4480 0.29 +0.2
3,945,402 - hdfR b4480 0.30 -1.1
3,945,402 - hdfR b4480 0.30 -1.7
3,945,543 + hdfR b4480 0.47 -1.7
3,945,543 + hdfR b4480 0.47 -0.0
3,945,551 - hdfR b4480 0.48 -1.5
3,945,744 - hdfR b4480 0.71 +1.0
3,945,783 + hdfR b4480 0.75 -0.6
3,945,783 + hdfR b4480 0.75 -0.8
3,945,897 + hdfR b4480 0.89 -0.5
3,945,902 + hdfR b4480 0.89 -1.9
3,945,914 - -1.1
3,946,030 - -1.4
3,946,030 - +0.2
3,946,045 + -2.1

Or see this region's nucleotide sequence