Strain Fitness in Escherichia coli BW25113 around b1831

Experiment: LB Anaerobic with Spiramycin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntprc and proQ are separated by 19 nucleotidesproQ and yebR are separated by 96 nucleotidesyebR and yebS are separated by 129 nucleotides b1830: prc - carboxy-terminal protease for penicillin-binding protein 3 (NCBI), at 1,910,792 to 1,912,840 prc b1831: proQ - putative solute/DNA competence effector (NCBI), at 1,912,860 to 1,913,558 proQ b1832: yebR - free methionine-(R)-sulfoxide reductase (RefSeq), at 1,913,655 to 1,914,152 yebR b1833: yebS - conserved inner membrane protein (NCBI), at 1,914,282 to 1,915,565 yebS Position (kb) 1912 1913 1914Strain fitness (log2 ratio) -2 -1 0 1at 1912.082 kb on + strand, within prcat 1912.123 kb on - strand, within prcat 1912.140 kb on + strand, within prcat 1912.140 kb on + strand, within prcat 1912.166 kb on + strand, within prcat 1912.166 kb on + strand, within prcat 1912.759 kb on + strandat 1912.759 kb on + strandat 1912.794 kb on - strandat 1912.972 kb on + strand, within proQat 1912.972 kb on + strand, within proQat 1913.231 kb on - strand, within proQat 1913.263 kb on - strand, within proQat 1913.263 kb on - strand, within proQat 1913.405 kb on + strand, within proQat 1913.405 kb on + strand, within proQat 1913.413 kb on - strand, within proQat 1913.725 kb on - strand, within yebRat 1913.752 kb on + strand, within yebRat 1913.752 kb on + strand, within yebRat 1913.760 kb on - strand, within yebRat 1913.790 kb on - strand, within yebRat 1913.790 kb on - strand, within yebRat 1913.864 kb on - strand, within yebRat 1913.864 kb on - strand, within yebRat 1913.964 kb on - strand, within yebRat 1913.964 kb on - strand, within yebRat 1913.965 kb on - strand, within yebRat 1913.984 kb on + strand, within yebRat 1914.464 kb on + strand, within yebS

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM
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1,912,082 + prc b1830 0.63 -1.8
1,912,123 - prc b1830 0.65 -1.8
1,912,140 + prc b1830 0.66 -0.8
1,912,140 + prc b1830 0.66 -1.1
1,912,166 + prc b1830 0.67 -1.9
1,912,166 + prc b1830 0.67 -1.3
1,912,759 + -2.3
1,912,759 + -2.0
1,912,794 - -1.3
1,912,972 + proQ b1831 0.16 -2.1
1,912,972 + proQ b1831 0.16 -1.1
1,913,231 - proQ b1831 0.53 -1.8
1,913,263 - proQ b1831 0.58 -1.8
1,913,263 - proQ b1831 0.58 -1.6
1,913,405 + proQ b1831 0.78 -1.3
1,913,405 + proQ b1831 0.78 +0.2
1,913,413 - proQ b1831 0.79 -1.6
1,913,725 - yebR b1832 0.14 +0.1
1,913,752 + yebR b1832 0.19 +1.0
1,913,752 + yebR b1832 0.19 +0.0
1,913,760 - yebR b1832 0.21 +0.4
1,913,790 - yebR b1832 0.27 +0.2
1,913,790 - yebR b1832 0.27 -0.7
1,913,864 - yebR b1832 0.42 -1.6
1,913,864 - yebR b1832 0.42 +0.6
1,913,964 - yebR b1832 0.62 +0.8
1,913,964 - yebR b1832 0.62 +1.5
1,913,965 - yebR b1832 0.62 -0.2
1,913,984 + yebR b1832 0.66 -0.8
1,914,464 + yebS b1833 0.14 +0.7

Or see this region's nucleotide sequence