Experiment: LB Anaerobic with Spiramycin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt crcA and cspE are separated by 174 nucleotides cspE and crcB are separated by 53 nucleotides crcB and ybeM are separated by 92 nucleotides ybeM and tatE are separated by 128 nucleotides tatE and lipA are separated by 100 nucleotides
b0622: crcA - palmitoyl transferase for Lipid A (NCBI), at 655,780 to 656,340
crcA
b0623: cspE - cold shock protein E (NCBI), at 656,515 to 656,724
cspE
b0624: crcB - camphor resistance protein CrcB (NCBI), at 656,778 to 657,161
crcB
b4581: ybeM - no description, at 657,254 to 658,041
ybeM
b0627: tatE - twin arginine translocase protein E (NCBI), at 658,170 to 658,373
tatE
b0628: lipA - lipoyl synthase (NCBI), at 658,474 to 659,439
lipA
Position (kb) 657
658
659 Strain fitness (log2 ratio) -1
0
1 at 656.284 kb on - strand, within crcA at 656.298 kb on + strand at 656.428 kb on + strand at 656.535 kb on + strand at 656.553 kb on + strand, within cspE at 656.648 kb on + strand, within cspE at 656.813 kb on + strand at 656.927 kb on + strand, within crcB at 656.935 kb on - strand, within crcB at 656.992 kb on + strand, within crcB at 657.065 kb on - strand, within crcB at 657.121 kb on + strand, within crcB at 657.208 kb on + strand at 657.425 kb on - strand, within ybeM at 657.782 kb on + strand, within ybeM at 657.836 kb on + strand, within ybeM at 657.836 kb on + strand, within ybeM at 657.836 kb on - strand, within ybeM at 657.898 kb on - strand, within ybeM at 657.917 kb on + strand, within ybeM at 657.945 kb on + strand, within ybeM at 657.963 kb on + strand at 657.963 kb on + strand at 658.011 kb on - strand at 658.163 kb on - strand at 658.218 kb on + strand, within tatE at 658.234 kb on - strand, within tatE at 658.234 kb on - strand, within tatE at 658.262 kb on + strand, within tatE at 658.262 kb on + strand, within tatE at 658.385 kb on + strand at 658.460 kb on - strand at 658.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM remove 656,284 - crcA b0622 0.90 -0.8 656,298 + -0.9 656,428 + -1.3 656,535 + +0.0 656,553 + cspE b0623 0.18 -1.3 656,648 + cspE b0623 0.63 -0.5 656,813 + +0.5 656,927 + crcB b0624 0.39 -0.2 656,935 - crcB b0624 0.41 +0.6 656,992 + crcB b0624 0.56 +1.0 657,065 - crcB b0624 0.75 -0.4 657,121 + crcB b0624 0.89 +1.0 657,208 + -0.2 657,425 - ybeM b4581 0.22 +0.7 657,782 + ybeM b4581 0.67 +0.2 657,836 + ybeM b4581 0.74 +0.2 657,836 + ybeM b4581 0.74 +1.3 657,836 - ybeM b4581 0.74 +0.6 657,898 - ybeM b4581 0.82 -0.1 657,917 + ybeM b4581 0.84 +0.2 657,945 + ybeM b4581 0.88 +0.3 657,963 + -0.2 657,963 + +1.1 658,011 - -1.3 658,163 - +0.1 658,218 + tatE b0627 0.24 +0.0 658,234 - tatE b0627 0.31 -1.1 658,234 - tatE b0627 0.31 -0.1 658,262 + tatE b0627 0.45 +0.5 658,262 + tatE b0627 0.45 -0.5 658,385 + +0.2 658,460 - -0.3 658,460 - +0.8
Or see this region's nucleotide sequence