Strain Fitness in Escherichia coli BW25113 around b3587

Experiment: LB Anaerobic with Spiramycin 0.00003125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Spiramycin 0.00003125 mM
3,751,147	+	yiaV	b3586	0.14	+1.0
3,751,202	+	yiaV	b3586	0.19	+1.4
3,751,202	+	yiaV	b3586	0.19	-0.4
3,751,256	-	yiaV	b3586	0.24	+0.4
3,751,362	+	yiaV	b3586	0.33	+0.4
3,751,385	-	yiaV	b3586	0.35	+0.2
3,751,401	+	yiaV	b3586	0.36	+0.6
3,751,418	+	yiaV	b3586	0.38	-0.4
3,751,418	+	yiaV	b3586	0.38	-0.3
3,751,423	+	yiaV	b3586	0.38	+0.9
3,751,440	+	yiaV	b3586	0.40	+1.1
3,751,440	+	yiaV	b3586	0.40	+0.6
3,751,467	-	yiaV	b3586	0.42	-0.5
3,751,534	+	yiaV	b3586	0.48	+0.0
3,751,534	+	yiaV	b3586	0.48	-0.3
3,751,534	+	yiaV	b3586	0.48	-0.3
3,751,535	+	yiaV	b3586	0.48	+0.4
3,751,542	-	yiaV	b3586	0.49	+0.6
3,751,542	-	yiaV	b3586	0.49	-1.5
3,751,586	+	yiaV	b3586	0.53	+0.0
3,751,586	+	yiaV	b3586	0.53	-0.3
3,751,683	+	yiaV	b3586	0.61	+0.6
3,751,683	+	yiaV	b3586	0.61	-0.8
3,751,683	-	yiaV	b3586	0.61	+0.8
3,751,683	-	yiaV	b3586	0.61	+0.4
3,751,691	-	yiaV	b3586	0.62	-1.0
3,751,691	-	yiaV	b3586	0.62	-0.5
3,751,699	-	yiaV	b3586	0.63	-1.5
3,751,699	-	yiaV	b3586	0.63	+0.5
3,751,739	-	yiaV	b3586	0.66	+0.4
3,751,747	+	yiaV	b3586	0.67	+0.4
3,751,751	-	yiaV	b3586	0.67	-0.1
3,751,756	-	yiaV	b3586	0.68	-0.3
3,751,756	-	yiaV	b3586	0.68	+0.1
3,751,756	-	yiaV	b3586	0.68	-0.1
3,751,772	+	yiaV	b3586	0.69	+0.2
3,751,772	+	yiaV	b3586	0.69	-0.4
3,751,780	-	yiaV	b3586	0.70	+0.4
3,751,783	+	yiaV	b3586	0.70	-1.5
3,751,790	+	yiaV	b3586	0.71	+1.4
3,751,805	+	yiaV	b3586	0.72	-0.0
3,751,822	+	yiaV	b3586	0.74	-0.4
3,751,822	+	yiaV	b3586	0.74	-0.3
3,751,842	+	yiaV	b3586	0.75	+0.6
3,751,842	+	yiaV	b3586	0.75	-0.4
3,751,843	+	yiaV	b3586	0.75	+0.4
3,751,851	-	yiaV	b3586	0.76	-0.9
3,751,863	+	yiaV	b3586	0.77	+0.0
3,751,863	+	yiaV	b3586	0.77	-1.1
3,751,871	-	yiaV	b3586	0.78	-0.6
3,751,896	+	yiaV	b3586	0.80	+0.6
3,751,896	+	yiaV	b3586	0.80	-1.6
3,751,898	+	yiaV	b3586	0.80	-0.5
3,751,900	+	yiaV	b3586	0.80	+0.4
3,751,904	-	yiaV	b3586	0.81	-0.5
3,751,908	-	yiaV	b3586	0.81	+0.1
3,751,908	-	yiaV	b3586	0.81	-0.6
3,751,910	+	yiaV	b3586	0.81	-0.4
3,751,910	+	yiaV	b3586	0.81	-0.2
3,751,910	+	yiaV	b3586	0.81	-1.4
3,751,910	+	yiaV	b3586	0.81	-1.4
3,751,914	-	yiaV	b3586	0.82	-0.1
3,751,915	+	yiaV	b3586	0.82	+1.4
3,751,918	-	yiaV	b3586	0.82	-0.8
3,751,918	-	yiaV	b3586	0.82	+0.4
3,751,919	-	yiaV	b3586	0.82	+2.0
3,751,919	-	yiaV	b3586	0.82	+0.6
3,751,919	-	yiaV	b3586	0.82	-1.0
3,751,921	+	yiaV	b3586	0.82	-0.2
3,751,924	-	yiaV	b3586	0.82	+1.0
3,751,924	-	yiaV	b3586	0.82	+2.0
3,751,961	+	yiaV	b3586	0.86	+0.1
3,751,961	+	yiaV	b3586	0.86	-0.9
3,751,961	+	yiaV	b3586	0.86	+1.6
3,751,962	+	yiaV	b3586	0.86	-1.8
3,751,969	+	yiaV	b3586	0.86	+0.1
3,751,969	+	yiaV	b3586	0.86	+2.0
3,751,974	-	yiaV	b3586	0.87	+1.0
3,752,012	+				+0.4
3,752,029	+				+2.0
3,752,039	-				+1.0
3,752,040	-				-0.3
3,752,040	-				+1.0
3,752,076	-				+0.0
3,752,077	-				-0.4
3,752,082	-				+1.7
3,752,098	-				-0.0
3,752,098	-				+1.6
3,752,125	-				+1.2
3,752,133	-				+0.2
3,752,180	-	yiaW	b3587	0.16	-0.5
3,752,180	-	yiaW	b3587	0.16	+0.7
3,752,185	-	yiaW	b3587	0.18	-0.7
3,752,185	-	yiaW	b3587	0.18	-1.0
3,752,195	-	yiaW	b3587	0.21	+0.5
3,752,195	-	yiaW	b3587	0.21	+0.1
3,752,208	+	yiaW	b3587	0.25	-0.4
3,752,230	+	yiaW	b3587	0.31	-2.4
3,752,230	+	yiaW	b3587	0.31	-1.0
3,752,230	+	yiaW	b3587	0.31	-1.6
3,752,230	+	yiaW	b3587	0.31	+1.0
3,752,230	+	yiaW	b3587	0.31	-0.4
3,752,230	+	yiaW	b3587	0.31	+2.0
3,752,230	+	yiaW	b3587	0.31	+0.4
3,752,230	+	yiaW	b3587	0.31	-0.0
3,752,231	-	yiaW	b3587	0.32	-1.2
3,752,234	+	yiaW	b3587	0.33	+2.0
3,752,238	-	yiaW	b3587	0.34	+1.0
3,752,238	-	yiaW	b3587	0.34	-1.4
3,752,238	-	yiaW	b3587	0.34	+0.8
3,752,238	-	yiaW	b3587	0.34	+2.0
3,752,238	-	yiaW	b3587	0.34	-1.3
3,752,238	-	yiaW	b3587	0.34	-1.5
3,752,240	+	yiaW	b3587	0.35	+2.0
3,752,245	+	yiaW	b3587	0.36	+0.4
3,752,255	+	yiaW	b3587	0.39	-0.7
3,752,255	+	yiaW	b3587	0.39	+1.0
3,752,291	+	yiaW	b3587	0.50	-0.8
3,752,298	-	yiaW	b3587	0.52	+2.0
3,752,302	+	yiaW	b3587	0.54	-1.0
3,752,329	+	yiaW	b3587	0.62	+2.0
3,752,330	+	yiaW	b3587	0.62	-0.4
3,752,330	+	yiaW	b3587	0.62	-0.8
3,752,334	+	yiaW	b3587	0.64	-1.0
3,752,392	+	yiaW	b3587	0.81	+2.0
3,752,547	-				-0.7
3,752,547	-				+0.1
3,752,650	-				+0.6
3,752,650	-				+0.0
3,752,661	+				+1.0
3,752,669	-				-2.0
3,752,669	-				-0.2
3,752,675	+				+2.0
3,752,683	-				-1.0
3,752,706	-				+2.0
3,752,706	-				+2.0
3,752,721	-				-0.0
3,752,721	-				+0.5
3,752,727	-				+0.2
3,752,734	+				-0.7
3,752,742	-				+0.0
3,752,742	-				+1.2
3,752,747	-				-0.2
3,752,771	+				-0.4
3,752,800	+				-0.0
3,752,817	+				+1.8
3,752,817	+				-0.3
3,752,821	+				+0.7
3,752,822	+				-0.3
3,752,822	+				+0.2
3,752,842	-				-0.1
3,752,877	+				+0.1
3,752,895	+				-0.8
3,752,900	+				-0.4
3,752,900	+				-0.6
3,752,903	-				-0.6
3,752,903	-				-0.1
3,752,903	-				-0.2
3,752,927	+				-0.2
3,752,927	+				-0.7
3,752,969	-				-1.2
3,752,970	-				-0.3
3,752,999	+				-0.5
3,753,018	+				-0.2
3,753,021	-				+0.4
3,753,027	+				-0.2
3,753,067	-				-0.8
3,753,071	+				-0.7
3,753,130	+				+1.5
3,753,130	+				+0.2
3,753,163	-	aldB	b3588	0.11	-0.3
3,753,174	-	aldB	b3588	0.12	-0.6
3,753,224	+	aldB	b3588	0.15	+0.1
3,753,373	-	aldB	b3588	0.24	+0.2
3,753,373	-	aldB	b3588	0.24	-0.3