Strain Fitness in Escherichia coli BW25113 around b3068

Experiment: LB Anaerobic with Spiramycin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoD and ygjF are separated by 78 nucleotidesygjF and ileX are separated by 124 nucleotidesileX and yqjH are separated by 53 nucleotides b3067: rpoD - RNA polymerase sigma factor (NCBI), at 3,211,069 to 3,212,910 rpoD b3068: ygjF - G/U mismatch-specific DNA glycosylase (NCBI), at 3,212,989 to 3,213,495 ygjF b3069: ileX - tRNA-Ile (NCBI), at 3,213,620 to 3,213,695 ileX b3070: yqjH - predicted siderophore interacting protein (NCBI), at 3,213,749 to 3,214,513 yqjH Position (kb) 3212 3213 3214Strain fitness (log2 ratio) -1 0 1at 3213.016 kb on + strandat 3213.058 kb on - strand, within ygjFat 3213.069 kb on - strand, within ygjFat 3213.276 kb on - strand, within ygjFat 3213.334 kb on + strand, within ygjFat 3213.334 kb on + strand, within ygjFat 3213.449 kb on - strandat 3213.497 kb on - strandat 3213.497 kb on - strandat 3213.692 kb on + strandat 3213.789 kb on + strandat 3213.868 kb on - strand, within yqjHat 3213.983 kb on - strand, within yqjHat 3213.995 kb on - strand, within yqjHat 3213.998 kb on - strand, within yqjHat 3213.998 kb on - strand, within yqjHat 3214.144 kb on + strand, within yqjHat 3214.168 kb on - strand, within yqjHat 3214.254 kb on - strand, within yqjHat 3214.289 kb on - strand, within yqjHat 3214.334 kb on - strand, within yqjHat 3214.334 kb on - strand, within yqjHat 3214.347 kb on + strand, within yqjHat 3214.383 kb on - strand, within yqjHat 3214.408 kb on - strand, within yqjHat 3214.440 kb on + strandat 3214.448 kb on - strandat 3214.485 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM
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3,213,016 + -0.1
3,213,058 - ygjF b3068 0.14 +1.1
3,213,069 - ygjF b3068 0.16 +0.7
3,213,276 - ygjF b3068 0.57 +1.2
3,213,334 + ygjF b3068 0.68 -0.4
3,213,334 + ygjF b3068 0.68 -0.4
3,213,449 - -0.0
3,213,497 - +0.4
3,213,497 - -0.3
3,213,692 + +0.3
3,213,789 + +1.0
3,213,868 - yqjH b3070 0.16 +0.1
3,213,983 - yqjH b3070 0.31 +0.5
3,213,995 - yqjH b3070 0.32 -1.2
3,213,998 - yqjH b3070 0.33 -0.7
3,213,998 - yqjH b3070 0.33 -1.4
3,214,144 + yqjH b3070 0.52 -0.2
3,214,168 - yqjH b3070 0.55 +0.4
3,214,254 - yqjH b3070 0.66 -0.1
3,214,289 - yqjH b3070 0.71 -0.8
3,214,334 - yqjH b3070 0.76 +0.1
3,214,334 - yqjH b3070 0.76 +0.3
3,214,347 + yqjH b3070 0.78 -0.2
3,214,383 - yqjH b3070 0.83 -0.1
3,214,408 - yqjH b3070 0.86 +0.3
3,214,440 + +0.2
3,214,448 - +0.5
3,214,485 + -0.7

Or see this region's nucleotide sequence