Experiment: LB Anaerobic with Spiramycin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gshA and yqaA are separated by 72 nucleotides yqaA and yqaB overlap by 4 nucleotides yqaB and argQ are separated by 280 nucleotides argQ and argZ are separated by 198 nucleotides argZ and argY are separated by 62 nucleotides argY and argV are separated by 198 nucleotides
b2688: gshA - glutamate--cysteine ligase (NCBI), at 2,812,905 to 2,814,461
gshA
b2689: yqaA - conserved inner membrane protein (NCBI), at 2,814,534 to 2,814,962
yqaA
b2690: yqaB - predicted hydrolase (NCBI), at 2,814,959 to 2,815,525
yqaB
b2691: argQ - tRNA-Arg (NCBI), at 2,815,806 to 2,815,882
argQ
b2692: argZ - tRNA-Arg (NCBI), at 2,816,081 to 2,816,157
argZ
b2693: argY - tRNA-Arg (NCBI), at 2,816,220 to 2,816,296
argY
b2694: argV - tRNA-Arg (NCBI), at 2,816,495 to 2,816,571
argV
Position (kb)
2814
2815
2816 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2814.108 kb on - strand, within gshA at 2814.115 kb on + strand, within gshA at 2814.131 kb on - strand, within gshA at 2814.131 kb on - strand, within gshA at 2814.139 kb on + strand, within gshA at 2814.139 kb on + strand, within gshA at 2814.144 kb on + strand, within gshA at 2814.191 kb on + strand, within gshA at 2814.233 kb on + strand, within gshA at 2814.233 kb on + strand, within gshA at 2814.303 kb on + strand, within gshA at 2814.308 kb on + strand at 2814.308 kb on + strand at 2814.316 kb on - strand at 2814.331 kb on - strand at 2814.373 kb on + strand at 2814.552 kb on + strand at 2814.552 kb on + strand at 2814.709 kb on - strand, within yqaA at 2814.709 kb on - strand, within yqaA at 2814.745 kb on - strand, within yqaA at 2814.798 kb on - strand, within yqaA at 2814.900 kb on + strand, within yqaA at 2814.900 kb on + strand, within yqaA at 2814.981 kb on - strand at 2814.981 kb on - strand at 2814.983 kb on + strand at 2814.983 kb on + strand at 2814.991 kb on + strand at 2815.035 kb on + strand, within yqaB at 2815.035 kb on + strand, within yqaB at 2815.051 kb on + strand, within yqaB at 2815.059 kb on + strand, within yqaB at 2815.152 kb on + strand, within yqaB at 2815.153 kb on + strand, within yqaB at 2815.194 kb on - strand, within yqaB at 2815.279 kb on + strand, within yqaB at 2815.381 kb on - strand, within yqaB at 2815.430 kb on + strand, within yqaB at 2815.430 kb on + strand, within yqaB at 2815.430 kb on + strand, within yqaB at 2815.460 kb on + strand, within yqaB at 2815.472 kb on + strand at 2815.478 kb on - strand at 2815.480 kb on - strand at 2815.514 kb on - strand at 2815.529 kb on - strand at 2815.922 kb on + strand at 2815.996 kb on + strand at 2816.067 kb on - strand at 2816.132 kb on + strand, within argZ at 2816.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM remove 2,814,108 - gshA b2688 0.77 +0.1 2,814,115 + gshA b2688 0.78 +0.9 2,814,131 - gshA b2688 0.79 +1.2 2,814,131 - gshA b2688 0.79 -0.8 2,814,139 + gshA b2688 0.79 +2.7 2,814,139 + gshA b2688 0.79 +1.2 2,814,144 + gshA b2688 0.80 +0.9 2,814,191 + gshA b2688 0.83 +0.7 2,814,233 + gshA b2688 0.85 -0.1 2,814,233 + gshA b2688 0.85 +2.9 2,814,303 + gshA b2688 0.90 +1.1 2,814,308 + -0.5 2,814,308 + +0.5 2,814,316 - +1.5 2,814,331 - +0.8 2,814,373 + +1.2 2,814,552 + +1.0 2,814,552 + +0.2 2,814,709 - yqaA b2689 0.41 -0.7 2,814,709 - yqaA b2689 0.41 -0.1 2,814,745 - yqaA b2689 0.49 -1.4 2,814,798 - yqaA b2689 0.62 -0.6 2,814,900 + yqaA b2689 0.85 +0.0 2,814,900 + yqaA b2689 0.85 +0.7 2,814,981 - +0.9 2,814,981 - -0.6 2,814,983 + -0.7 2,814,983 + +0.8 2,814,991 + -0.1 2,815,035 + yqaB b2690 0.13 +0.8 2,815,035 + yqaB b2690 0.13 +2.6 2,815,051 + yqaB b2690 0.16 +0.2 2,815,059 + yqaB b2690 0.18 +0.1 2,815,152 + yqaB b2690 0.34 +1.9 2,815,153 + yqaB b2690 0.34 -0.2 2,815,194 - yqaB b2690 0.41 -1.0 2,815,279 + yqaB b2690 0.56 -1.1 2,815,381 - yqaB b2690 0.74 +0.2 2,815,430 + yqaB b2690 0.83 +1.5 2,815,430 + yqaB b2690 0.83 -0.0 2,815,430 + yqaB b2690 0.83 -0.8 2,815,460 + yqaB b2690 0.88 +0.7 2,815,472 + +0.3 2,815,478 - -0.5 2,815,480 - -2.5 2,815,514 - -0.4 2,815,529 - +1.5 2,815,922 + +0.5 2,815,996 + -0.1 2,816,067 - -0.4 2,816,132 + argZ b2692 0.66 -0.1 2,816,388 - -0.2
Or see this region's nucleotide sequence