Strain Fitness in Escherichia coli BW25113 around b2113

Experiment: LB Anaerobic with Spiramycin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyehD and yehE are separated by 292 nucleotidesyehE and mrp are separated by 262 nucleotidesmrp and metG are separated by 131 nucleotides b2111: yehD - predicted fimbrial-like adhesin protein (NCBI), at 2,189,702 to 2,190,244 yehD b2112: yehE - hypothetical protein (NCBI), at 2,190,537 to 2,190,818 yehE b2113: mrp - putative ATPase (VIMSS), at 2,191,081 to 2,192,190 mrp b2114: metG - methionyl-tRNA synthetase (NCBI), at 2,192,322 to 2,194,355 metG Position (kb) 2191 2192 2193Strain fitness (log2 ratio) -2 -1 0 1at 2190.294 kb on - strandat 2190.302 kb on - strandat 2190.520 kb on - strandat 2190.566 kb on - strand, within yehEat 2190.567 kb on - strand, within yehEat 2190.581 kb on + strand, within yehEat 2190.587 kb on - strand, within yehEat 2190.587 kb on - strand, within yehEat 2190.587 kb on - strand, within yehEat 2190.595 kb on - strand, within yehEat 2190.595 kb on - strand, within yehEat 2190.598 kb on - strand, within yehEat 2190.598 kb on - strand, within yehEat 2190.601 kb on - strandat 2190.608 kb on - strand, within yehEat 2190.608 kb on - strand, within yehEat 2190.610 kb on - strand, within yehEat 2190.610 kb on - strand, within yehEat 2190.610 kb on - strand, within yehEat 2190.637 kb on - strand, within yehEat 2190.637 kb on - strand, within yehEat 2190.641 kb on + strand, within yehEat 2190.643 kb on + strand, within yehEat 2190.644 kb on - strand, within yehEat 2190.648 kb on + strand, within yehEat 2190.649 kb on + strand, within yehEat 2190.651 kb on - strand, within yehEat 2190.651 kb on - strand, within yehEat 2190.651 kb on - strand, within yehEat 2190.668 kb on + strand, within yehEat 2190.677 kb on - strand, within yehEat 2190.677 kb on - strand, within yehEat 2190.679 kb on - strand, within yehEat 2190.680 kb on + strand, within yehEat 2190.682 kb on + strand, within yehEat 2190.690 kb on - strand, within yehEat 2190.694 kb on + strand, within yehEat 2190.695 kb on - strand, within yehEat 2190.696 kb on - strand, within yehEat 2190.697 kb on + strand, within yehEat 2190.700 kb on - strand, within yehEat 2190.700 kb on - strand, within yehEat 2190.700 kb on - strand, within yehEat 2190.704 kb on + strand, within yehEat 2190.705 kb on + strand, within yehEat 2190.708 kb on - strand, within yehEat 2190.710 kb on - strand, within yehEat 2190.711 kb on - strand, within yehEat 2190.712 kb on - strand, within yehEat 2190.715 kb on - strand, within yehEat 2190.715 kb on - strand, within yehEat 2190.739 kb on - strand, within yehEat 2190.756 kb on + strand, within yehEat 2190.817 kb on + strandat 2190.825 kb on - strandat 2190.830 kb on - strandat 2190.839 kb on - strandat 2190.839 kb on - strandat 2190.840 kb on + strandat 2190.844 kb on - strandat 2190.854 kb on - strandat 2190.860 kb on - strandat 2190.872 kb on - strandat 2190.872 kb on - strandat 2190.906 kb on + strandat 2190.906 kb on + strandat 2190.906 kb on - strandat 2190.906 kb on - strandat 2190.933 kb on - strandat 2190.933 kb on - strandat 2190.941 kb on + strandat 2190.993 kb on - strandat 2191.047 kb on - strandat 2191.073 kb on - strandat 2191.139 kb on + strandat 2191.284 kb on - strand, within mrpat 2191.284 kb on - strand, within mrpat 2191.307 kb on - strand, within mrpat 2191.307 kb on - strand, within mrpat 2191.329 kb on + strand, within mrpat 2191.329 kb on + strand, within mrpat 2191.334 kb on + strand, within mrpat 2191.345 kb on + strand, within mrpat 2191.450 kb on + strand, within mrpat 2191.689 kb on - strand, within mrpat 2191.689 kb on - strand, within mrpat 2191.755 kb on - strand, within mrpat 2191.810 kb on + strand, within mrpat 2191.923 kb on - strandat 2191.961 kb on + strand, within mrpat 2191.989 kb on - strand, within mrpat 2192.094 kb on + strandat 2192.094 kb on + strandat 2192.201 kb on + strandat 2192.251 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM
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2,190,294 - +0.3
2,190,302 - +1.3
2,190,520 - +0.9
2,190,566 - yehE b2112 0.10 -1.1
2,190,567 - yehE b2112 0.11 +0.3
2,190,581 + yehE b2112 0.16 +1.3
2,190,587 - yehE b2112 0.18 +0.4
2,190,587 - yehE b2112 0.18 -0.0
2,190,587 - yehE b2112 0.18 -0.6
2,190,595 - yehE b2112 0.21 +0.4
2,190,595 - yehE b2112 0.21 +0.4
2,190,598 - yehE b2112 0.22 +0.6
2,190,598 - yehE b2112 0.22 -0.1
2,190,601 - +0.3
2,190,608 - yehE b2112 0.25 +0.8
2,190,608 - yehE b2112 0.25 +0.1
2,190,610 - yehE b2112 0.26 -0.2
2,190,610 - yehE b2112 0.26 +0.4
2,190,610 - yehE b2112 0.26 +0.2
2,190,637 - yehE b2112 0.35 -0.0
2,190,637 - yehE b2112 0.35 +0.7
2,190,641 + yehE b2112 0.37 +1.3
2,190,643 + yehE b2112 0.38 +1.3
2,190,644 - yehE b2112 0.38 -0.3
2,190,648 + yehE b2112 0.39 +1.3
2,190,649 + yehE b2112 0.40 +1.3
2,190,651 - yehE b2112 0.40 -0.2
2,190,651 - yehE b2112 0.40 +1.3
2,190,651 - yehE b2112 0.40 +0.3
2,190,668 + yehE b2112 0.46 +1.3
2,190,677 - yehE b2112 0.50 -0.1
2,190,677 - yehE b2112 0.50 +0.3
2,190,679 - yehE b2112 0.50 -0.4
2,190,680 + yehE b2112 0.51 -0.7
2,190,682 + yehE b2112 0.51 +1.3
2,190,690 - yehE b2112 0.54 +0.2
2,190,694 + yehE b2112 0.56 +0.3
2,190,695 - yehE b2112 0.56 -0.2
2,190,696 - yehE b2112 0.56 -0.5
2,190,697 + yehE b2112 0.57 -2.2
2,190,700 - yehE b2112 0.58 +0.4
2,190,700 - yehE b2112 0.58 -0.5
2,190,700 - yehE b2112 0.58 -0.6
2,190,704 + yehE b2112 0.59 +0.3
2,190,705 + yehE b2112 0.60 +0.2
2,190,708 - yehE b2112 0.61 +0.3
2,190,710 - yehE b2112 0.61 +0.1
2,190,711 - yehE b2112 0.62 -1.3
2,190,712 - yehE b2112 0.62 +0.4
2,190,715 - yehE b2112 0.63 +0.3
2,190,715 - yehE b2112 0.63 -2.1
2,190,739 - yehE b2112 0.72 -0.4
2,190,756 + yehE b2112 0.78 -0.1
2,190,817 + -1.0
2,190,825 - -0.6
2,190,830 - +0.3
2,190,839 - -0.9
2,190,839 - -0.4
2,190,840 + -0.0
2,190,844 - -0.7
2,190,854 - -0.6
2,190,860 - -1.0
2,190,872 - -1.0
2,190,872 - +0.1
2,190,906 + -0.5
2,190,906 + -0.3
2,190,906 - -0.4
2,190,906 - +0.3
2,190,933 - +0.2
2,190,933 - +0.6
2,190,941 + -0.0
2,190,993 - +0.0
2,191,047 - -0.4
2,191,073 - -0.3
2,191,139 + +0.1
2,191,284 - mrp b2113 0.18 -0.9
2,191,284 - mrp b2113 0.18 +1.3
2,191,307 - mrp b2113 0.20 +0.7
2,191,307 - mrp b2113 0.20 +0.5
2,191,329 + mrp b2113 0.22 +1.6
2,191,329 + mrp b2113 0.22 +0.9
2,191,334 + mrp b2113 0.23 +1.2
2,191,345 + mrp b2113 0.24 -0.0
2,191,450 + mrp b2113 0.33 -0.5
2,191,689 - mrp b2113 0.55 -0.0
2,191,689 - mrp b2113 0.55 -0.0
2,191,755 - mrp b2113 0.61 +0.6
2,191,810 + mrp b2113 0.66 -0.4
2,191,923 - -0.1
2,191,961 + mrp b2113 0.79 +0.2
2,191,989 - mrp b2113 0.82 +1.2
2,192,094 + +0.1
2,192,094 + -0.7
2,192,201 + -0.2
2,192,251 - -0.6

Or see this region's nucleotide sequence