Experiment: LB Anaerobic with Spiramycin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydaU and ydaV are separated by 6 nucleotides ydaV and ydaW overlap by 29 nucleotides ydaW and rzpR are separated by 0 nucleotides rzpR and rzoR overlap by 245 nucleotides rzoR and trkG are separated by 196 nucleotides
b1359: ydaU - Rac prophage; conserved protein (NCBI), at 1,419,143 to 1,420,000
ydaU
b1360: ydaV - Rac prophage; predicted DNA replication protein (NCBI), at 1,420,007 to 1,420,753
ydaV
b1361: ydaW - orf, hypothetical protein (VIMSS), at 1,420,725 to 1,421,224
ydaW
b1362: rzpR - putative Rac prophage endopeptidase (VIMSS), at 1,421,225 to 1,421,668
rzpR
b4528: rzoR - Rac prophage; predicted lipoprotein (NCBI), at 1,421,424 to 1,421,609
rzoR
b1363: trkG - Rac prophage; potassium transporter subunit (NCBI), at 1,421,806 to 1,423,263
trkG
Position (kb)
1420
1421
1422 Strain fitness (log2 ratio)
-1
0
1
2 at 1419.797 kb on + strand, within ydaU at 1419.910 kb on + strand, within ydaU at 1419.933 kb on - strand at 1419.988 kb on - strand at 1420.068 kb on - strand at 1420.436 kb on - strand, within ydaV at 1420.589 kb on + strand, within ydaV at 1420.831 kb on - strand, within ydaW at 1421.276 kb on - strand, within rzpR at 1421.536 kb on + strand at 1421.536 kb on + strand at 1421.602 kb on + strand at 1421.612 kb on + strand, within rzpR at 1421.612 kb on + strand, within rzpR at 1421.623 kb on + strand, within rzpR at 1421.623 kb on + strand, within rzpR at 1421.638 kb on + strand at 1421.638 kb on + strand at 1421.641 kb on + strand at 1421.672 kb on + strand at 1421.672 kb on + strand at 1421.813 kb on + strand at 1421.838 kb on + strand at 1421.838 kb on + strand at 1421.841 kb on + strand at 1421.841 kb on + strand at 1421.846 kb on + strand at 1421.847 kb on + strand at 1421.848 kb on + strand at 1421.848 kb on + strand at 1421.859 kb on + strand at 1421.875 kb on + strand at 1421.891 kb on + strand at 1421.895 kb on + strand at 1421.983 kb on + strand, within trkG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM remove 1,419,797 + ydaU b1359 0.76 +0.9 1,419,910 + ydaU b1359 0.89 -0.8 1,419,933 - +0.2 1,419,988 - +0.2 1,420,068 - -0.3 1,420,436 - ydaV b1360 0.57 -0.8 1,420,589 + ydaV b1360 0.78 -0.1 1,420,831 - ydaW b1361 0.21 -0.9 1,421,276 - rzpR b1362 0.11 +0.2 1,421,536 + -0.2 1,421,536 + -0.1 1,421,602 + -0.3 1,421,612 + rzpR b1362 0.87 +0.7 1,421,612 + rzpR b1362 0.87 +0.5 1,421,623 + rzpR b1362 0.90 +0.4 1,421,623 + rzpR b1362 0.90 +0.5 1,421,638 + +0.9 1,421,638 + -1.1 1,421,641 + +1.1 1,421,672 + +0.7 1,421,672 + -0.3 1,421,813 + +1.2 1,421,838 + +1.2 1,421,838 + -1.6 1,421,841 + +2.6 1,421,841 + -0.3 1,421,846 + -0.3 1,421,847 + -0.2 1,421,848 + -0.6 1,421,848 + -0.3 1,421,859 + +1.2 1,421,875 + -0.3 1,421,891 + -0.8 1,421,895 + +0.2 1,421,983 + trkG b1363 0.12 +1.2
Or see this region's nucleotide sequence