Strain Fitness in Escherichia coli BW25113 around b1241

Experiment: LB Anaerobic with Spiramycin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsZ and adhE are separated by 453 nucleotidesadhE and ychE are separated by 476 nucleotides b4573: insZ - no description, at 1,293,649 to 1,294,215 insZ b1241: adhE - fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI), at 1,294,669 to 1,297,344 adhE b1242: ychE - predicted inner membrane protein (NCBI), at 1,297,821 to 1,298,468 ychE Position (kb) 1294 1295 1296 1297 1298Strain fitness (log2 ratio) -2 -1 0 1at 1293.750 kb on + strand, within insZat 1293.800 kb on - strand, within insZat 1293.965 kb on + strand, within insZat 1294.028 kb on - strand, within insZat 1294.043 kb on - strand, within insZat 1294.043 kb on - strand, within insZat 1294.144 kb on - strand, within insZat 1294.175 kb on - strandat 1294.207 kb on + strandat 1294.444 kb on - strandat 1294.479 kb on + strandat 1294.487 kb on - strandat 1294.487 kb on - strandat 1294.504 kb on - strandat 1294.504 kb on - strandat 1294.512 kb on + strandat 1294.770 kb on + strandat 1294.793 kb on - strandat 1294.928 kb on - strandat 1294.933 kb on - strandat 1294.971 kb on - strand, within adhEat 1294.979 kb on - strand, within adhEat 1294.998 kb on - strand, within adhEat 1295.050 kb on - strand, within adhEat 1295.128 kb on - strand, within adhEat 1295.128 kb on - strand, within adhEat 1295.139 kb on + strand, within adhEat 1295.139 kb on + strand, within adhEat 1295.179 kb on + strand, within adhEat 1295.294 kb on + strand, within adhEat 1295.410 kb on - strand, within adhEat 1295.430 kb on - strand, within adhEat 1295.564 kb on - strand, within adhEat 1295.708 kb on + strand, within adhEat 1295.708 kb on + strand, within adhEat 1295.762 kb on - strand, within adhEat 1295.918 kb on + strand, within adhEat 1296.102 kb on - strand, within adhEat 1296.102 kb on - strand, within adhEat 1296.107 kb on + strand, within adhEat 1296.107 kb on + strand, within adhEat 1296.113 kb on + strand, within adhEat 1296.113 kb on + strand, within adhEat 1296.135 kb on - strand, within adhEat 1296.695 kb on - strand, within adhEat 1296.705 kb on + strand, within adhEat 1296.705 kb on + strand, within adhEat 1296.997 kb on - strand, within adhEat 1297.301 kb on + strandat 1297.311 kb on - strandat 1297.349 kb on - strandat 1297.349 kb on - strandat 1297.391 kb on + strandat 1297.500 kb on + strandat 1297.500 kb on + strandat 1297.531 kb on - strandat 1297.531 kb on - strandat 1297.558 kb on - strandat 1297.750 kb on - strandat 1297.787 kb on - strandat 1297.936 kb on - strand, within ychEat 1298.207 kb on - strand, within ychEat 1298.242 kb on - strand, within ychEat 1298.242 kb on - strand, within ychEat 1298.286 kb on - strand, within ychEat 1298.300 kb on + strand, within ychEat 1298.334 kb on - strand, within ychE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM
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1,293,750 + insZ b4573 0.18 -0.7
1,293,800 - insZ b4573 0.27 +0.0
1,293,965 + insZ b4573 0.56 -0.2
1,294,028 - insZ b4573 0.67 -0.5
1,294,043 - insZ b4573 0.69 +1.5
1,294,043 - insZ b4573 0.69 +0.5
1,294,144 - insZ b4573 0.87 -0.5
1,294,175 - +0.9
1,294,207 + -0.3
1,294,444 - -0.3
1,294,479 + -0.2
1,294,487 - +0.6
1,294,487 - +0.3
1,294,504 - -0.4
1,294,504 - -0.1
1,294,512 + -0.6
1,294,770 + -1.4
1,294,793 - -0.6
1,294,928 - -0.7
1,294,933 - -0.5
1,294,971 - adhE b1241 0.11 -1.5
1,294,979 - adhE b1241 0.12 +0.1
1,294,998 - adhE b1241 0.12 -1.2
1,295,050 - adhE b1241 0.14 -1.0
1,295,128 - adhE b1241 0.17 -1.8
1,295,128 - adhE b1241 0.17 -1.1
1,295,139 + adhE b1241 0.18 -1.6
1,295,139 + adhE b1241 0.18 -0.5
1,295,179 + adhE b1241 0.19 -0.6
1,295,294 + adhE b1241 0.23 -1.1
1,295,410 - adhE b1241 0.28 -0.1
1,295,430 - adhE b1241 0.28 -0.2
1,295,564 - adhE b1241 0.33 -0.7
1,295,708 + adhE b1241 0.39 +0.5
1,295,708 + adhE b1241 0.39 -2.7
1,295,762 - adhE b1241 0.41 -0.2
1,295,918 + adhE b1241 0.47 -0.2
1,296,102 - adhE b1241 0.54 -1.5
1,296,102 - adhE b1241 0.54 -0.6
1,296,107 + adhE b1241 0.54 -1.8
1,296,107 + adhE b1241 0.54 -1.0
1,296,113 + adhE b1241 0.54 -0.6
1,296,113 + adhE b1241 0.54 -0.8
1,296,135 - adhE b1241 0.55 -2.1
1,296,695 - adhE b1241 0.76 -0.8
1,296,705 + adhE b1241 0.76 -1.3
1,296,705 + adhE b1241 0.76 -0.5
1,296,997 - adhE b1241 0.87 -0.9
1,297,301 + -0.3
1,297,311 - -0.6
1,297,349 - -0.8
1,297,349 - -0.9
1,297,391 + -0.7
1,297,500 + -0.5
1,297,500 + -0.0
1,297,531 - -0.6
1,297,531 - -0.3
1,297,558 - -0.3
1,297,750 - +0.3
1,297,787 - +0.2
1,297,936 - ychE b1242 0.18 -1.3
1,298,207 - ychE b1242 0.60 +0.5
1,298,242 - ychE b1242 0.65 +0.1
1,298,242 - ychE b1242 0.65 -0.3
1,298,286 - ychE b1242 0.72 +0.3
1,298,300 + ychE b1242 0.74 +0.3
1,298,334 - ychE b1242 0.79 -1.9

Or see this region's nucleotide sequence