Experiment: LB Anaerobic with Spiramycin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispA and xseB overlap by 1 nucleotides xseB and thiI are separated by 205 nucleotides thiI and thiJ are separated by 53 nucleotides thiJ and panE overlap by 38 nucleotides
b0421: ispA - geranyltranstransferase (NCBI), at 439,426 to 440,325
ispA
b0422: xseB - exodeoxyribonuclease VII small subunit (NCBI), at 440,325 to 440,567
xseB
b0423: thiI - thiamine biosynthesis protein ThiI (NCBI), at 440,773 to 442,221
thiI
b0424: thiJ - 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis (VIMSS), at 442,275 to 442,865
thiJ
b0425: panE - 2-dehydropantoate 2-reductase (NCBI), at 442,828 to 443,739
panE
Position (kb) 440
441
442
443 Strain fitness (log2 ratio) -2
-1
0
1
2 at 440.335 kb on - strand at 440.546 kb on - strand at 440.581 kb on - strand at 440.581 kb on - strand at 440.609 kb on + strand at 440.650 kb on + strand at 440.650 kb on + strand at 440.650 kb on - strand at 440.716 kb on - strand at 440.750 kb on - strand at 440.882 kb on + strand at 440.882 kb on + strand at 440.882 kb on + strand at 440.890 kb on - strand at 440.890 kb on - strand at 440.968 kb on + strand, within thiI at 440.968 kb on + strand, within thiI at 440.976 kb on - strand, within thiI at 440.976 kb on - strand, within thiI at 440.976 kb on - strand, within thiI at 441.031 kb on - strand, within thiI at 441.031 kb on - strand, within thiI at 441.040 kb on - strand, within thiI at 441.066 kb on - strand, within thiI at 441.100 kb on + strand, within thiI at 441.430 kb on - strand, within thiI at 441.471 kb on + strand, within thiI at 441.575 kb on - strand, within thiI at 441.575 kb on - strand, within thiI at 441.603 kb on + strand, within thiI at 441.605 kb on - strand, within thiI at 441.711 kb on - strand, within thiI at 441.723 kb on - strand, within thiI at 441.723 kb on - strand, within thiI at 442.032 kb on + strand, within thiI at 442.079 kb on + strand at 442.111 kb on + strand at 442.111 kb on + strand at 442.126 kb on + strand at 442.169 kb on + strand at 442.169 kb on + strand at 442.173 kb on + strand at 442.173 kb on + strand at 442.175 kb on + strand at 442.183 kb on + strand at 442.189 kb on + strand at 442.189 kb on + strand at 442.191 kb on - strand at 442.197 kb on - strand at 442.197 kb on - strand at 442.207 kb on - strand at 442.267 kb on + strand at 442.267 kb on + strand at 442.267 kb on + strand at 442.345 kb on - strand, within thiJ at 442.385 kb on + strand, within thiJ at 442.387 kb on + strand, within thiJ at 442.425 kb on + strand, within thiJ at 442.438 kb on + strand, within thiJ at 442.514 kb on - strand, within thiJ at 442.576 kb on - strand, within thiJ at 442.576 kb on - strand, within thiJ at 442.660 kb on - strand, within thiJ at 442.660 kb on - strand, within thiJ at 442.668 kb on + strand, within thiJ at 442.684 kb on + strand, within thiJ at 442.717 kb on - strand, within thiJ at 442.796 kb on - strand, within thiJ at 442.814 kb on + strand at 442.823 kb on + strand at 442.823 kb on + strand at 442.831 kb on - strand at 442.848 kb on - strand at 442.848 kb on - strand at 442.853 kb on - strand at 442.902 kb on - strand at 442.917 kb on - strand at 443.001 kb on - strand, within panE at 443.091 kb on - strand, within panE at 443.139 kb on + strand, within panE at 443.147 kb on - strand, within panE at 443.147 kb on - strand, within panE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM remove 440,335 - -0.4 440,546 - -0.1 440,581 - -0.6 440,581 - -1.6 440,609 + +0.5 440,650 + -0.0 440,650 + +0.3 440,650 - -0.3 440,716 - +1.5 440,750 - +0.3 440,882 + -0.1 440,882 + -0.2 440,882 + -1.8 440,890 - +0.0 440,890 - -0.5 440,968 + thiI b0423 0.13 -2.1 440,968 + thiI b0423 0.13 -0.7 440,976 - thiI b0423 0.14 -1.1 440,976 - thiI b0423 0.14 -1.5 440,976 - thiI b0423 0.14 -0.4 441,031 - thiI b0423 0.18 +1.5 441,031 - thiI b0423 0.18 -0.5 441,040 - thiI b0423 0.18 +1.7 441,066 - thiI b0423 0.20 -1.0 441,100 + thiI b0423 0.23 -0.3 441,430 - thiI b0423 0.45 -1.5 441,471 + thiI b0423 0.48 -2.0 441,575 - thiI b0423 0.55 -1.7 441,575 - thiI b0423 0.55 -0.4 441,603 + thiI b0423 0.57 -1.5 441,605 - thiI b0423 0.57 -1.9 441,711 - thiI b0423 0.65 -2.2 441,723 - thiI b0423 0.66 +0.2 441,723 - thiI b0423 0.66 +0.5 442,032 + thiI b0423 0.87 -1.5 442,079 + +0.2 442,111 + -0.8 442,111 + +0.1 442,126 + -1.4 442,169 + +1.6 442,169 + +0.0 442,173 + -0.3 442,173 + -0.3 442,175 + -0.2 442,183 + -1.1 442,189 + -0.9 442,189 + -1.0 442,191 - -0.1 442,197 - -0.8 442,197 - -1.4 442,207 - -1.7 442,267 + +1.5 442,267 + -1.6 442,267 + +0.3 442,345 - thiJ b0424 0.12 +0.0 442,385 + thiJ b0424 0.19 +0.7 442,387 + thiJ b0424 0.19 +0.8 442,425 + thiJ b0424 0.25 +0.3 442,438 + thiJ b0424 0.28 -0.1 442,514 - thiJ b0424 0.40 -0.5 442,576 - thiJ b0424 0.51 -0.0 442,576 - thiJ b0424 0.51 -1.0 442,660 - thiJ b0424 0.65 -0.4 442,660 - thiJ b0424 0.65 +2.5 442,668 + thiJ b0424 0.66 +0.3 442,684 + thiJ b0424 0.69 -1.7 442,717 - thiJ b0424 0.75 -0.0 442,796 - thiJ b0424 0.88 +0.1 442,814 + +0.5 442,823 + -0.8 442,823 + +0.5 442,831 - +0.0 442,848 - -1.7 442,848 - -0.2 442,853 - +0.2 442,902 - +0.4 442,917 - +0.2 443,001 - panE b0425 0.19 +0.2 443,091 - panE b0425 0.29 -0.4 443,139 + panE b0425 0.34 -0.1 443,147 - panE b0425 0.35 +0.7 443,147 - panE b0425 0.35 -0.1
Or see this region's nucleotide sequence