Strain Fitness in Escherichia coli BW25113 around b4699

Experiment: LB Anaerobic with Spiramycin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydaN and fnrS are separated by 95 nucleotidesfnrS and dbpA are separated by 260 nucleotides b1342: ydaN - zinc transporter (NCBI), at 1,406,074 to 1,407,057 ydaN b4699: fnrS - FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq), at 1,407,153 to 1,407,274 fnrS b1343: dbpA - ATP-dependent RNA helicase, specific for 23S rRNA (NCBI), at 1,407,535 to 1,408,908 dbpA Position (kb) 1407 1408Strain fitness (log2 ratio) -1 0 1at 1406.243 kb on + strand, within ydaNat 1406.396 kb on + strand, within ydaNat 1406.441 kb on + strand, within ydaNat 1406.441 kb on + strand, within ydaNat 1406.471 kb on + strand, within ydaNat 1406.479 kb on - strand, within ydaNat 1406.521 kb on + strand, within ydaNat 1406.521 kb on + strand, within ydaNat 1406.622 kb on + strand, within ydaNat 1406.678 kb on - strand, within ydaNat 1406.727 kb on + strand, within ydaNat 1406.727 kb on + strand, within ydaNat 1406.783 kb on + strand, within ydaNat 1406.812 kb on - strand, within ydaNat 1406.814 kb on - strand, within ydaNat 1406.822 kb on - strand, within ydaNat 1406.876 kb on + strand, within ydaNat 1406.879 kb on - strand, within ydaNat 1406.879 kb on - strand, within ydaNat 1407.150 kb on - strandat 1407.225 kb on - strand, within fnrSat 1407.299 kb on - strandat 1407.384 kb on + strandat 1407.384 kb on + strandat 1407.421 kb on + strandat 1407.429 kb on - strandat 1407.799 kb on + strand, within dbpAat 1408.018 kb on + strand, within dbpAat 1408.018 kb on + strand, within dbpAat 1408.236 kb on + strand, within dbpAat 1408.267 kb on - strand, within dbpA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.00003125 mM
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1,406,243 + ydaN b1342 0.17 -0.1
1,406,396 + ydaN b1342 0.33 -0.0
1,406,441 + ydaN b1342 0.37 +0.4
1,406,441 + ydaN b1342 0.37 +0.8
1,406,471 + ydaN b1342 0.40 +0.4
1,406,479 - ydaN b1342 0.41 -0.6
1,406,521 + ydaN b1342 0.45 -0.6
1,406,521 + ydaN b1342 0.45 +0.1
1,406,622 + ydaN b1342 0.56 +1.1
1,406,678 - ydaN b1342 0.61 -1.3
1,406,727 + ydaN b1342 0.66 +0.0
1,406,727 + ydaN b1342 0.66 +0.1
1,406,783 + ydaN b1342 0.72 +0.0
1,406,812 - ydaN b1342 0.75 -0.4
1,406,814 - ydaN b1342 0.75 +0.2
1,406,822 - ydaN b1342 0.76 +0.2
1,406,876 + ydaN b1342 0.82 +0.6
1,406,879 - ydaN b1342 0.82 +0.8
1,406,879 - ydaN b1342 0.82 +1.2
1,407,150 - +0.0
1,407,225 - fnrS b4699 0.59 -0.6
1,407,299 - -0.4
1,407,384 + +0.2
1,407,384 + +1.2
1,407,421 + +0.6
1,407,429 - -0.6
1,407,799 + dbpA b1343 0.19 -0.8
1,408,018 + dbpA b1343 0.35 +0.2
1,408,018 + dbpA b1343 0.35 -0.5
1,408,236 + dbpA b1343 0.51 -0.0
1,408,267 - dbpA b1343 0.53 +0.1

Or see this region's nucleotide sequence