Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dicC and dicA are separated by 83 nucleotides dicA and ydfA are separated by 166 nucleotides ydfA and ydfB are separated by 1 nucleotides ydfB and ydfC are separated by 29 nucleotides ydfC and dicF are separated by 340 nucleotides dicF and dicB are separated by 174 nucleotides dicB and ydfD overlap by 4 nucleotides
b1569: dicC - Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI), at 1,645,644 to 1,645,874
dicC
b1570: dicA - Qin prophage; predicted regulator for DicB (NCBI), at 1,645,958 to 1,646,365
dicA
b1571: ydfA - Qin prophage; predicted protein (NCBI), at 1,646,532 to 1,646,687
ydfA
b1572: ydfB - orf, hypothetical protein (VIMSS), at 1,646,689 to 1,646,817
ydfB
b1573: ydfC - Qin prophage; predicted protein (RefSeq), at 1,646,847 to 1,647,065
ydfC
b1574: dicF - antisense RNA DicF (NCBI), at 1,647,406 to 1,647,458
dicF
b1575: dicB - Qin prophage; cell division inhibition protein (NCBI), at 1,647,633 to 1,647,821
dicB
b1576: ydfD - Qin prophage; predicted protein (NCBI), at 1,647,818 to 1,648,009
ydfD
Position (kb)
1646
1647
1648 Strain fitness (log2 ratio)
-2
-1
0
1 at 1645.940 kb on + strand at 1646.751 kb on - strand, within ydfB at 1646.887 kb on - strand, within ydfC at 1646.997 kb on - strand, within ydfC at 1647.366 kb on - strand at 1647.500 kb on - strand at 1647.653 kb on + strand, within dicB at 1647.700 kb on + strand, within dicB at 1647.709 kb on + strand, within dicB at 1647.775 kb on - strand, within dicB at 1647.880 kb on - strand, within ydfD at 1647.900 kb on - strand, within ydfD at 1647.957 kb on - strand, within ydfD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 1,645,940 + +0.1 1,646,751 - ydfB b1572 0.48 -0.2 1,646,887 - ydfC b1573 0.18 +0.9 1,646,997 - ydfC b1573 0.68 +0.4 1,647,366 - -0.2 1,647,500 - +0.4 1,647,653 + dicB b1575 0.11 -1.6 1,647,700 + dicB b1575 0.35 -0.2 1,647,709 + dicB b1575 0.40 -2.2 1,647,775 - dicB b1575 0.75 +1.4 1,647,880 - ydfD b1576 0.32 +1.4 1,647,900 - ydfD b1576 0.43 +1.4 1,647,957 - ydfD b1576 0.72 +1.4
Or see this region's nucleotide sequence