Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potG and potH are separated by 9 nucleotides potH and potI overlap by 4 nucleotides potI and ybjO are separated by 59 nucleotides
b0855: potG - ATP-binding component of putrescine transport system (VIMSS), at 894,214 to 895,347
potG
b0856: potH - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 895,357 to 896,310
potH
b0857: potI - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 896,307 to 897,152
potI
b0858: ybjO - predicted inner membrane protein (NCBI), at 897,212 to 897,700
ybjO
Position (kb) 895
896
897 Strain fitness (log2 ratio) -2
-1
0
1 at 894.396 kb on + strand, within potG at 894.541 kb on - strand, within potG at 894.691 kb on - strand, within potG at 894.746 kb on - strand, within potG at 894.755 kb on + strand, within potG at 894.755 kb on + strand, within potG at 894.797 kb on - strand, within potG at 894.816 kb on + strand, within potG at 894.847 kb on - strand, within potG at 894.877 kb on + strand, within potG at 894.922 kb on + strand, within potG at 894.922 kb on + strand, within potG at 894.929 kb on - strand, within potG at 895.067 kb on + strand, within potG at 895.099 kb on - strand, within potG at 895.099 kb on - strand, within potG at 895.246 kb on + strand at 895.325 kb on + strand at 895.325 kb on + strand at 895.461 kb on + strand, within potH at 895.461 kb on + strand, within potH at 895.603 kb on - strand, within potH at 895.694 kb on + strand, within potH at 895.848 kb on + strand, within potH at 895.861 kb on - strand, within potH at 895.930 kb on + strand, within potH at 895.937 kb on - strand, within potH at 895.937 kb on - strand, within potH at 896.095 kb on - strand, within potH at 896.425 kb on + strand, within potI at 896.425 kb on + strand, within potI at 896.429 kb on + strand, within potI at 896.429 kb on + strand, within potI at 896.497 kb on - strand, within potI at 896.497 kb on - strand, within potI at 896.607 kb on - strand, within potI at 896.607 kb on - strand, within potI at 897.041 kb on + strand, within potI at 897.053 kb on - strand, within potI at 897.084 kb on - strand at 897.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 894,396 + potG b0855 0.16 -0.4 894,541 - potG b0855 0.29 -0.1 894,691 - potG b0855 0.42 -0.5 894,746 - potG b0855 0.47 +0.3 894,755 + potG b0855 0.48 -2.1 894,755 + potG b0855 0.48 -1.3 894,797 - potG b0855 0.51 +0.3 894,816 + potG b0855 0.53 -0.0 894,847 - potG b0855 0.56 +0.8 894,877 + potG b0855 0.58 -0.2 894,922 + potG b0855 0.62 -0.5 894,922 + potG b0855 0.62 -0.1 894,929 - potG b0855 0.63 +0.7 895,067 + potG b0855 0.75 -0.2 895,099 - potG b0855 0.78 -0.5 895,099 - potG b0855 0.78 +0.1 895,246 + +0.8 895,325 + +0.8 895,325 + +0.5 895,461 + potH b0856 0.11 +0.4 895,461 + potH b0856 0.11 +1.4 895,603 - potH b0856 0.26 +0.8 895,694 + potH b0856 0.35 +1.1 895,848 + potH b0856 0.51 +0.3 895,861 - potH b0856 0.53 +0.6 895,930 + potH b0856 0.60 +0.3 895,937 - potH b0856 0.61 +0.1 895,937 - potH b0856 0.61 +0.9 896,095 - potH b0856 0.77 -0.1 896,425 + potI b0857 0.14 +0.3 896,425 + potI b0857 0.14 +0.8 896,429 + potI b0857 0.14 -0.2 896,429 + potI b0857 0.14 -0.6 896,497 - potI b0857 0.22 +0.1 896,497 - potI b0857 0.22 +0.5 896,607 - potI b0857 0.35 +1.5 896,607 - potI b0857 0.35 -0.2 897,041 + potI b0857 0.87 +0.2 897,053 - potI b0857 0.88 -0.2 897,084 - -0.6 897,084 - -0.1
Or see this region's nucleotide sequence