Strain Fitness in Escherichia coli BW25113 around b0175

Experiment: LB Anaerobic with Spiramycin 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdxr and ispU are separated by 185 nucleotidesispU and cdsA are separated by 12 nucleotidescdsA and yaeL are separated by 11 nucleotides b0173: dxr - 1-deoxy-D-xylulose 5-phosphate reductoisomerase (NCBI), at 193,521 to 194,717 dxr b0174: ispU - undecaprenyl pyrophosphate synthase (NCBI), at 194,903 to 195,664 ispU b0175: cdsA - CDP-diglyceride synthetase (VIMSS), at 195,677 to 196,534 cdsA b0176: yaeL - zinc metallopeptidase (NCBI), at 196,546 to 197,898 yaeL Position (kb) 195 196 197Strain fitness (log2 ratio) -1 0 1 2 3at 194.726 kb on + strandat 194.748 kb on + strandat 194.767 kb on + strandat 194.767 kb on + strandat 194.767 kb on + strandat 194.767 kb on + strandat 194.803 kb on + strandat 194.803 kb on + strandat 194.872 kb on + strandat 194.872 kb on + strandat 194.872 kb on + strandat 195.682 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM
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194,726 + -0.7
194,748 + +0.6
194,767 + +0.7
194,767 + -0.2
194,767 + -1.0
194,767 + -0.3
194,803 + -0.1
194,803 + -0.6
194,872 + -0.1
194,872 + +0.8
194,872 + -1.4
195,682 + +3.1

Or see this region's nucleotide sequence