Experiment: KB with Quercetin 360 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3416 and Psyr_3417 are separated by 125 nucleotides Psyr_3417 and Psyr_3418 overlap by 1 nucleotides Psyr_3418 and Psyr_3419 are separated by 64 nucleotides Psyr_3419 and Psyr_3420 are separated by 10 nucleotides Psyr_3420 and Psyr_3421 are separated by 2 nucleotides
Psyr_3416: Psyr_3416 - Cytochrome c oxidase cbb3-type, subunit III, at 4,080,189 to 4,081,166
_3416
Psyr_3417: Psyr_3417 - Prevent-host-death protein, at 4,081,292 to 4,081,543
_3417
Psyr_3418: Psyr_3418 - PilT protein, N-terminal, at 4,081,543 to 4,081,995
_3418
Psyr_3419: Psyr_3419 - 4Fe-4S ferredoxin, iron-sulfur binding protein, at 4,082,060 to 4,083,472
_3419
Psyr_3420: Psyr_3420 - conserved hypothetical protein, at 4,083,483 to 4,084,019
_3420
Psyr_3421: Psyr_3421 - Copper-translocating P-type ATPase:Heavy metal translocating P-type ATPase, at 4,084,022 to 4,086,496
_3421
Position (kb) 4082
4083
4084 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4081.261 kb on - strand at 4081.269 kb on + strand at 4081.269 kb on + strand at 4081.269 kb on + strand at 4081.270 kb on - strand at 4081.421 kb on + strand, within Psyr_3417 at 4081.421 kb on + strand, within Psyr_3417 at 4081.681 kb on - strand, within Psyr_3418 at 4081.748 kb on + strand, within Psyr_3418 at 4081.748 kb on + strand, within Psyr_3418 at 4081.748 kb on + strand, within Psyr_3418 at 4081.748 kb on + strand, within Psyr_3418 at 4081.748 kb on + strand, within Psyr_3418 at 4081.749 kb on - strand, within Psyr_3418 at 4081.749 kb on - strand, within Psyr_3418 at 4081.749 kb on - strand, within Psyr_3418 at 4081.749 kb on - strand, within Psyr_3418 at 4081.749 kb on - strand, within Psyr_3418 at 4081.749 kb on - strand, within Psyr_3418 at 4081.975 kb on + strand at 4082.129 kb on + strand at 4082.130 kb on - strand at 4082.385 kb on - strand, within Psyr_3419 at 4082.411 kb on - strand, within Psyr_3419 at 4082.690 kb on + strand, within Psyr_3419 at 4082.691 kb on - strand, within Psyr_3419 at 4082.691 kb on - strand, within Psyr_3419 at 4082.695 kb on + strand, within Psyr_3419 at 4082.696 kb on - strand, within Psyr_3419 at 4082.722 kb on - strand, within Psyr_3419 at 4082.722 kb on - strand, within Psyr_3419 at 4082.722 kb on - strand, within Psyr_3419 at 4082.729 kb on + strand, within Psyr_3419 at 4082.729 kb on + strand, within Psyr_3419 at 4082.730 kb on - strand, within Psyr_3419 at 4082.730 kb on - strand, within Psyr_3419 at 4082.792 kb on + strand, within Psyr_3419 at 4082.997 kb on - strand, within Psyr_3419 at 4083.018 kb on - strand, within Psyr_3419 at 4083.254 kb on + strand, within Psyr_3419 at 4083.463 kb on + strand at 4083.463 kb on + strand at 4083.464 kb on - strand at 4083.627 kb on + strand, within Psyr_3420 at 4083.627 kb on + strand, within Psyr_3420 at 4083.627 kb on + strand, within Psyr_3420 at 4083.627 kb on + strand, within Psyr_3420 at 4083.627 kb on + strand, within Psyr_3420 at 4083.627 kb on + strand, within Psyr_3420 at 4083.627 kb on + strand, within Psyr_3420 at 4083.628 kb on - strand, within Psyr_3420 at 4083.628 kb on - strand, within Psyr_3420 at 4083.630 kb on + strand, within Psyr_3420 at 4084.058 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Quercetin 360 ug/ml remove 4,081,261 - +0.1 4,081,269 + -0.5 4,081,269 + -1.1 4,081,269 + +1.2 4,081,270 - +0.2 4,081,421 + Psyr_3417 0.51 +1.1 4,081,421 + Psyr_3417 0.51 -1.2 4,081,681 - Psyr_3418 0.30 -0.6 4,081,748 + Psyr_3418 0.45 -0.2 4,081,748 + Psyr_3418 0.45 -0.9 4,081,748 + Psyr_3418 0.45 -0.6 4,081,748 + Psyr_3418 0.45 -0.2 4,081,748 + Psyr_3418 0.45 -2.2 4,081,749 - Psyr_3418 0.45 -0.1 4,081,749 - Psyr_3418 0.45 +2.1 4,081,749 - Psyr_3418 0.45 -1.0 4,081,749 - Psyr_3418 0.45 -0.0 4,081,749 - Psyr_3418 0.45 -2.7 4,081,749 - Psyr_3418 0.45 +1.4 4,081,975 + -0.2 4,082,129 + +0.4 4,082,130 - +2.2 4,082,385 - Psyr_3419 0.23 -0.0 4,082,411 - Psyr_3419 0.25 -0.6 4,082,690 + Psyr_3419 0.45 -0.0 4,082,691 - Psyr_3419 0.45 -1.3 4,082,691 - Psyr_3419 0.45 -0.2 4,082,695 + Psyr_3419 0.45 -2.0 4,082,696 - Psyr_3419 0.45 +1.0 4,082,722 - Psyr_3419 0.47 -0.1 4,082,722 - Psyr_3419 0.47 -1.3 4,082,722 - Psyr_3419 0.47 -3.3 4,082,729 + Psyr_3419 0.47 -1.3 4,082,729 + Psyr_3419 0.47 -1.3 4,082,730 - Psyr_3419 0.47 +1.0 4,082,730 - Psyr_3419 0.47 -0.6 4,082,792 + Psyr_3419 0.52 -2.5 4,082,997 - Psyr_3419 0.66 +2.0 4,083,018 - Psyr_3419 0.68 -2.0 4,083,254 + Psyr_3419 0.85 -1.3 4,083,463 + -1.6 4,083,463 + +0.4 4,083,464 - -2.4 4,083,627 + Psyr_3420 0.27 +0.4 4,083,627 + Psyr_3420 0.27 -0.1 4,083,627 + Psyr_3420 0.27 +0.4 4,083,627 + Psyr_3420 0.27 -0.6 4,083,627 + Psyr_3420 0.27 -1.3 4,083,627 + Psyr_3420 0.27 -0.3 4,083,627 + Psyr_3420 0.27 +0.8 4,083,628 - Psyr_3420 0.27 +0.6 4,083,628 - Psyr_3420 0.27 +0.3 4,083,630 + Psyr_3420 0.27 +0.8 4,084,058 + +0.2
Or see this region's nucleotide sequence