Experiment: LB Aerobic with Ramoplanin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yggL and yggH overlap by 1 nucleotides yggH and mutY are separated by 160 nucleotides mutY and yggX are separated by 27 nucleotides yggX and mltC are separated by 64 nucleotides
b2959: yggL - orf, hypothetical protein (VIMSS), at 3,099,829 to 3,100,155
yggL
b2960: yggH - tRNA(m7G46)-methyltransferase (NCBI), at 3,100,155 to 3,100,874
yggH
b2961: mutY - adenine DNA glycosylase (NCBI), at 3,101,035 to 3,102,087
mutY
b2962: yggX - hypothetical protein (NCBI), at 3,102,115 to 3,102,390
yggX
b2963: mltC - membrane-bound lytic murein transglycosylase C (VIMSS), at 3,102,455 to 3,103,534
mltC
Position (kb)
3101
3102
3103 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3100.069 kb on - strand, within yggL at 3100.069 kb on - strand, within yggL at 3100.096 kb on + strand, within yggL at 3100.181 kb on + strand at 3100.187 kb on - strand at 3100.187 kb on - strand at 3100.189 kb on - strand at 3100.189 kb on - strand at 3100.250 kb on + strand, within yggH at 3100.250 kb on + strand, within yggH at 3100.258 kb on - strand, within yggH at 3100.258 kb on - strand, within yggH at 3100.258 kb on - strand, within yggH at 3100.260 kb on - strand, within yggH at 3100.393 kb on - strand, within yggH at 3100.406 kb on - strand, within yggH at 3100.441 kb on + strand, within yggH at 3100.441 kb on + strand, within yggH at 3100.474 kb on + strand, within yggH at 3100.521 kb on - strand, within yggH at 3100.521 kb on - strand, within yggH at 3100.568 kb on - strand, within yggH at 3100.600 kb on + strand, within yggH at 3100.600 kb on + strand, within yggH at 3100.616 kb on - strand, within yggH at 3100.827 kb on - strand at 3100.829 kb on - strand at 3100.974 kb on + strand at 3100.974 kb on + strand at 3100.974 kb on + strand at 3101.012 kb on - strand at 3101.070 kb on - strand at 3101.070 kb on - strand at 3101.122 kb on + strand at 3101.122 kb on + strand at 3101.124 kb on + strand at 3101.124 kb on + strand at 3101.251 kb on + strand, within mutY at 3101.251 kb on + strand, within mutY at 3101.259 kb on + strand, within mutY at 3101.259 kb on + strand, within mutY at 3101.430 kb on + strand, within mutY at 3101.430 kb on + strand, within mutY at 3101.456 kb on - strand, within mutY at 3101.462 kb on - strand, within mutY at 3101.462 kb on - strand, within mutY at 3101.483 kb on - strand, within mutY at 3101.483 kb on - strand, within mutY at 3101.483 kb on - strand, within mutY at 3101.483 kb on - strand, within mutY at 3101.561 kb on + strand, within mutY at 3101.605 kb on + strand, within mutY at 3101.620 kb on + strand, within mutY at 3101.811 kb on - strand, within mutY at 3101.911 kb on + strand, within mutY at 3101.942 kb on + strand, within mutY at 3102.040 kb on + strand at 3102.152 kb on + strand, within yggX at 3102.350 kb on - strand, within yggX at 3102.350 kb on - strand, within yggX at 3102.352 kb on + strand, within yggX at 3102.352 kb on + strand, within yggX at 3102.352 kb on - strand, within yggX at 3102.443 kb on - strand at 3102.604 kb on + strand, within mltC at 3102.604 kb on + strand, within mltC at 3102.612 kb on - strand, within mltC at 3102.622 kb on - strand, within mltC at 3102.623 kb on - strand, within mltC at 3102.623 kb on - strand, within mltC at 3102.631 kb on - strand, within mltC at 3102.636 kb on + strand, within mltC at 3102.636 kb on + strand, within mltC at 3102.636 kb on + strand, within mltC at 3102.638 kb on + strand, within mltC at 3102.638 kb on + strand, within mltC at 3102.644 kb on - strand, within mltC at 3102.644 kb on - strand, within mltC at 3102.646 kb on - strand, within mltC at 3102.648 kb on + strand, within mltC at 3102.648 kb on + strand, within mltC at 3102.676 kb on - strand, within mltC at 3102.694 kb on + strand, within mltC at 3102.700 kb on - strand, within mltC at 3102.700 kb on - strand, within mltC at 3102.729 kb on + strand, within mltC at 3102.729 kb on + strand, within mltC at 3102.737 kb on - strand, within mltC at 3102.737 kb on - strand, within mltC at 3102.789 kb on + strand, within mltC at 3102.816 kb on - strand, within mltC at 3102.858 kb on + strand, within mltC at 3102.868 kb on + strand, within mltC at 3102.960 kb on - strand, within mltC at 3102.964 kb on + strand, within mltC at 3102.964 kb on + strand, within mltC at 3102.973 kb on + strand, within mltC at 3102.974 kb on - strand, within mltC at 3102.974 kb on - strand, within mltC at 3102.985 kb on + strand, within mltC at 3102.985 kb on + strand, within mltC at 3102.992 kb on - strand, within mltC at 3102.995 kb on - strand, within mltC at 3103.017 kb on - strand, within mltC at 3103.024 kb on + strand, within mltC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Ramoplanin 0.000125 mM remove 3,100,069 - yggL b2959 0.73 -0.2 3,100,069 - yggL b2959 0.73 -1.8 3,100,096 + yggL b2959 0.82 -1.5 3,100,181 + -2.0 3,100,187 - -2.4 3,100,187 - -1.9 3,100,189 - -0.8 3,100,189 - -2.5 3,100,250 + yggH b2960 0.13 +0.9 3,100,250 + yggH b2960 0.13 +0.3 3,100,258 - yggH b2960 0.14 -2.9 3,100,258 - yggH b2960 0.14 -1.9 3,100,258 - yggH b2960 0.14 -1.0 3,100,260 - yggH b2960 0.15 -0.9 3,100,393 - yggH b2960 0.33 -2.3 3,100,406 - yggH b2960 0.35 -2.6 3,100,441 + yggH b2960 0.40 -1.9 3,100,441 + yggH b2960 0.40 -2.5 3,100,474 + yggH b2960 0.44 -1.7 3,100,521 - yggH b2960 0.51 -2.3 3,100,521 - yggH b2960 0.51 -1.2 3,100,568 - yggH b2960 0.57 -1.9 3,100,600 + yggH b2960 0.62 -2.0 3,100,600 + yggH b2960 0.62 -1.7 3,100,616 - yggH b2960 0.64 -1.3 3,100,827 - -0.8 3,100,829 - -1.8 3,100,974 + -0.6 3,100,974 + +2.4 3,100,974 + -0.4 3,101,012 - +0.2 3,101,070 - -2.2 3,101,070 - -1.0 3,101,122 + -1.6 3,101,122 + +0.8 3,101,124 + +0.1 3,101,124 + +1.8 3,101,251 + mutY b2961 0.21 -0.1 3,101,251 + mutY b2961 0.21 +0.2 3,101,259 + mutY b2961 0.21 -1.1 3,101,259 + mutY b2961 0.21 -0.8 3,101,430 + mutY b2961 0.38 -0.9 3,101,430 + mutY b2961 0.38 -0.1 3,101,456 - mutY b2961 0.40 +0.4 3,101,462 - mutY b2961 0.41 +0.7 3,101,462 - mutY b2961 0.41 -0.8 3,101,483 - mutY b2961 0.43 -0.4 3,101,483 - mutY b2961 0.43 +0.4 3,101,483 - mutY b2961 0.43 +0.7 3,101,483 - mutY b2961 0.43 -0.5 3,101,561 + mutY b2961 0.50 +0.8 3,101,605 + mutY b2961 0.54 +1.1 3,101,620 + mutY b2961 0.56 +2.9 3,101,811 - mutY b2961 0.74 +1.0 3,101,911 + mutY b2961 0.83 +1.4 3,101,942 + mutY b2961 0.86 +0.2 3,102,040 + -0.0 3,102,152 + yggX b2962 0.13 +1.2 3,102,350 - yggX b2962 0.85 -1.6 3,102,350 - yggX b2962 0.85 -0.9 3,102,352 + yggX b2962 0.86 +0.2 3,102,352 + yggX b2962 0.86 +0.3 3,102,352 - yggX b2962 0.86 -0.1 3,102,443 - -0.8 3,102,604 + mltC b2963 0.14 +0.1 3,102,604 + mltC b2963 0.14 -1.9 3,102,612 - mltC b2963 0.15 -2.0 3,102,622 - mltC b2963 0.15 -0.1 3,102,623 - mltC b2963 0.16 -0.6 3,102,623 - mltC b2963 0.16 -1.3 3,102,631 - mltC b2963 0.16 -1.3 3,102,636 + mltC b2963 0.17 -0.9 3,102,636 + mltC b2963 0.17 -0.9 3,102,636 + mltC b2963 0.17 -0.5 3,102,638 + mltC b2963 0.17 -0.1 3,102,638 + mltC b2963 0.17 -0.9 3,102,644 - mltC b2963 0.17 +0.3 3,102,644 - mltC b2963 0.17 -0.7 3,102,646 - mltC b2963 0.18 -1.9 3,102,648 + mltC b2963 0.18 -3.2 3,102,648 + mltC b2963 0.18 -0.6 3,102,676 - mltC b2963 0.20 -1.3 3,102,694 + mltC b2963 0.22 -2.3 3,102,700 - mltC b2963 0.23 +0.7 3,102,700 - mltC b2963 0.23 -1.9 3,102,729 + mltC b2963 0.25 -0.5 3,102,729 + mltC b2963 0.25 -0.8 3,102,737 - mltC b2963 0.26 +0.3 3,102,737 - mltC b2963 0.26 -1.2 3,102,789 + mltC b2963 0.31 +0.1 3,102,816 - mltC b2963 0.33 -0.6 3,102,858 + mltC b2963 0.37 -1.3 3,102,868 + mltC b2963 0.38 +0.9 3,102,960 - mltC b2963 0.47 -0.8 3,102,964 + mltC b2963 0.47 -0.9 3,102,964 + mltC b2963 0.47 -0.3 3,102,973 + mltC b2963 0.48 -0.5 3,102,974 - mltC b2963 0.48 -0.8 3,102,974 - mltC b2963 0.48 -1.7 3,102,985 + mltC b2963 0.49 -1.6 3,102,985 + mltC b2963 0.49 -2.5 3,102,992 - mltC b2963 0.50 -0.8 3,102,995 - mltC b2963 0.50 -1.5 3,103,017 - mltC b2963 0.52 -0.2 3,103,024 + mltC b2963 0.53 -1.7
Or see this region's nucleotide sequence