Experiment: LB Aerobic with Ramoplanin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insH-7 and wbbK are separated by 479 nucleotides wbbK and wbbJ overlap by 16 nucleotides wbbJ and wbbI overlap by 20 nucleotides
b2030: insH-7 - IS5 transposase and trans-activator (NCBI), at 2,099,919 to 2,100,935
insH-7
b2032: wbbK - lipopolysaccharide biosynthesis protein (NCBI), at 2,101,415 to 2,102,533
wbbK
b2033: wbbJ - predicted acyl transferase (NCBI), at 2,102,518 to 2,103,108
wbbJ
b2034: wbbI - hypothetical protein (NCBI), at 2,103,089 to 2,104,081
wbbI
Position (kb)
2101
2102
2103 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2101.063 kb on + strand at 2101.087 kb on - strand at 2101.138 kb on + strand at 2101.139 kb on + strand at 2101.204 kb on - strand at 2101.204 kb on - strand at 2101.253 kb on - strand at 2101.253 kb on - strand at 2101.257 kb on - strand at 2101.263 kb on - strand at 2101.263 kb on - strand at 2101.284 kb on - strand at 2101.296 kb on - strand at 2101.330 kb on - strand at 2101.338 kb on - strand at 2101.339 kb on - strand at 2101.385 kb on - strand at 2101.386 kb on - strand at 2101.600 kb on - strand, within wbbK at 2101.637 kb on - strand, within wbbK at 2101.638 kb on - strand, within wbbK at 2101.651 kb on - strand, within wbbK at 2101.657 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.672 kb on - strand, within wbbK at 2101.791 kb on - strand, within wbbK at 2101.827 kb on - strand, within wbbK at 2101.827 kb on - strand, within wbbK at 2101.832 kb on - strand, within wbbK at 2101.832 kb on - strand, within wbbK at 2101.833 kb on - strand, within wbbK at 2101.921 kb on - strand, within wbbK at 2101.921 kb on - strand, within wbbK at 2101.950 kb on - strand, within wbbK at 2101.961 kb on - strand, within wbbK at 2102.023 kb on - strand, within wbbK at 2102.023 kb on - strand, within wbbK at 2102.032 kb on - strand, within wbbK at 2102.134 kb on - strand, within wbbK at 2102.134 kb on - strand, within wbbK at 2102.166 kb on - strand, within wbbK at 2102.166 kb on - strand, within wbbK at 2102.211 kb on - strand, within wbbK at 2102.211 kb on - strand, within wbbK at 2102.211 kb on - strand, within wbbK at 2102.211 kb on - strand, within wbbK at 2102.242 kb on - strand, within wbbK at 2102.242 kb on - strand, within wbbK at 2102.253 kb on - strand, within wbbK at 2102.280 kb on - strand, within wbbK at 2102.350 kb on - strand, within wbbK at 2102.351 kb on - strand, within wbbK at 2102.358 kb on - strand, within wbbK at 2102.406 kb on - strand, within wbbK at 2102.414 kb on - strand, within wbbK at 2102.418 kb on - strand, within wbbK at 2102.500 kb on - strand at 2102.587 kb on - strand, within wbbJ at 2102.589 kb on - strand, within wbbJ at 2102.640 kb on - strand, within wbbJ at 2102.686 kb on - strand, within wbbJ at 2102.694 kb on - strand, within wbbJ at 2102.702 kb on - strand, within wbbJ at 2102.769 kb on - strand, within wbbJ at 2102.769 kb on - strand, within wbbJ at 2102.783 kb on - strand, within wbbJ at 2102.828 kb on - strand, within wbbJ at 2102.828 kb on - strand, within wbbJ at 2102.833 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.871 kb on - strand, within wbbJ at 2102.876 kb on - strand, within wbbJ at 2102.933 kb on - strand, within wbbJ at 2102.939 kb on - strand, within wbbJ at 2102.939 kb on - strand, within wbbJ at 2102.939 kb on - strand, within wbbJ at 2102.996 kb on - strand, within wbbJ at 2103.057 kb on - strand at 2103.059 kb on - strand at 2103.116 kb on - strand at 2103.126 kb on - strand at 2103.126 kb on - strand at 2103.126 kb on - strand at 2103.137 kb on - strand at 2103.215 kb on - strand, within wbbI at 2103.215 kb on - strand, within wbbI at 2103.215 kb on - strand, within wbbI at 2103.215 kb on - strand, within wbbI at 2103.242 kb on - strand, within wbbI at 2103.251 kb on - strand, within wbbI at 2103.251 kb on - strand, within wbbI at 2103.252 kb on - strand, within wbbI at 2103.349 kb on - strand, within wbbI at 2103.362 kb on - strand, within wbbI at 2103.382 kb on - strand, within wbbI at 2103.382 kb on - strand, within wbbI at 2103.392 kb on - strand, within wbbI at 2103.393 kb on - strand, within wbbI at 2103.436 kb on - strand, within wbbI at 2103.436 kb on - strand, within wbbI at 2103.449 kb on - strand, within wbbI at 2103.490 kb on - strand, within wbbI at 2103.490 kb on - strand, within wbbI
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Ramoplanin 0.000125 mM remove 2,101,063 + +1.5 2,101,087 - +2.1 2,101,138 + -2.0 2,101,139 + +1.5 2,101,204 - +0.7 2,101,204 - -0.1 2,101,253 - -0.1 2,101,253 - +2.5 2,101,257 - +0.8 2,101,263 - +0.5 2,101,263 - -1.5 2,101,284 - -1.5 2,101,296 - -0.5 2,101,330 - -1.1 2,101,338 - +0.8 2,101,339 - +1.2 2,101,385 - -1.3 2,101,386 - -0.5 2,101,600 - wbbK b2032 0.17 -1.5 2,101,637 - wbbK b2032 0.20 +0.8 2,101,638 - wbbK b2032 0.20 -1.0 2,101,651 - wbbK b2032 0.21 +2.8 2,101,657 - wbbK b2032 0.22 -0.1 2,101,659 - wbbK b2032 0.22 -0.1 2,101,659 - wbbK b2032 0.22 +1.0 2,101,659 - wbbK b2032 0.22 -1.1 2,101,659 - wbbK b2032 0.22 -1.1 2,101,659 - wbbK b2032 0.22 -0.7 2,101,670 - wbbK b2032 0.23 +0.1 2,101,670 - wbbK b2032 0.23 +0.9 2,101,670 - wbbK b2032 0.23 -1.5 2,101,670 - wbbK b2032 0.23 +0.5 2,101,670 - wbbK b2032 0.23 -0.5 2,101,672 - wbbK b2032 0.23 -0.3 2,101,791 - wbbK b2032 0.34 +1.1 2,101,827 - wbbK b2032 0.37 -0.5 2,101,827 - wbbK b2032 0.37 +0.5 2,101,832 - wbbK b2032 0.37 -1.3 2,101,832 - wbbK b2032 0.37 -1.5 2,101,833 - wbbK b2032 0.37 -0.2 2,101,921 - wbbK b2032 0.45 +0.3 2,101,921 - wbbK b2032 0.45 -0.2 2,101,950 - wbbK b2032 0.48 -1.1 2,101,961 - wbbK b2032 0.49 -0.3 2,102,023 - wbbK b2032 0.54 +0.5 2,102,023 - wbbK b2032 0.54 -0.5 2,102,032 - wbbK b2032 0.55 +0.5 2,102,134 - wbbK b2032 0.64 +0.0 2,102,134 - wbbK b2032 0.64 -2.0 2,102,166 - wbbK b2032 0.67 +1.2 2,102,166 - wbbK b2032 0.67 -0.8 2,102,211 - wbbK b2032 0.71 -0.1 2,102,211 - wbbK b2032 0.71 -0.5 2,102,211 - wbbK b2032 0.71 -0.5 2,102,211 - wbbK b2032 0.71 -2.1 2,102,242 - wbbK b2032 0.74 -1.3 2,102,242 - wbbK b2032 0.74 -1.8 2,102,253 - wbbK b2032 0.75 -0.7 2,102,280 - wbbK b2032 0.77 -1.1 2,102,350 - wbbK b2032 0.84 -1.8 2,102,351 - wbbK b2032 0.84 -0.5 2,102,358 - wbbK b2032 0.84 +1.0 2,102,406 - wbbK b2032 0.89 -1.0 2,102,414 - wbbK b2032 0.89 -0.3 2,102,418 - wbbK b2032 0.90 -0.7 2,102,500 - +0.2 2,102,587 - wbbJ b2033 0.12 -1.3 2,102,589 - wbbJ b2033 0.12 -1.1 2,102,640 - wbbJ b2033 0.21 -1.7 2,102,686 - wbbJ b2033 0.28 -2.1 2,102,694 - wbbJ b2033 0.30 -1.1 2,102,702 - wbbJ b2033 0.31 +2.8 2,102,769 - wbbJ b2033 0.42 +1.6 2,102,769 - wbbJ b2033 0.42 +0.5 2,102,783 - wbbJ b2033 0.45 +0.8 2,102,828 - wbbJ b2033 0.52 -0.5 2,102,828 - wbbJ b2033 0.52 -2.0 2,102,833 - wbbJ b2033 0.53 -0.2 2,102,848 - wbbJ b2033 0.56 -1.0 2,102,848 - wbbJ b2033 0.56 -1.7 2,102,848 - wbbJ b2033 0.56 -0.1 2,102,848 - wbbJ b2033 0.56 +0.3 2,102,848 - wbbJ b2033 0.56 -1.2 2,102,848 - wbbJ b2033 0.56 +0.0 2,102,848 - wbbJ b2033 0.56 -0.8 2,102,871 - wbbJ b2033 0.60 +0.0 2,102,876 - wbbJ b2033 0.61 -2.0 2,102,933 - wbbJ b2033 0.70 -1.2 2,102,939 - wbbJ b2033 0.71 -0.5 2,102,939 - wbbJ b2033 0.71 -0.5 2,102,939 - wbbJ b2033 0.71 +0.8 2,102,996 - wbbJ b2033 0.81 -1.3 2,103,057 - -0.2 2,103,059 - +2.1 2,103,116 - +1.6 2,103,126 - -1.7 2,103,126 - +0.0 2,103,126 - -0.9 2,103,137 - -0.4 2,103,215 - wbbI b2034 0.13 +0.2 2,103,215 - wbbI b2034 0.13 -1.1 2,103,215 - wbbI b2034 0.13 +0.3 2,103,215 - wbbI b2034 0.13 -3.0 2,103,242 - wbbI b2034 0.15 -0.6 2,103,251 - wbbI b2034 0.16 -0.8 2,103,251 - wbbI b2034 0.16 -1.5 2,103,252 - wbbI b2034 0.16 +0.4 2,103,349 - wbbI b2034 0.26 -1.5 2,103,362 - wbbI b2034 0.27 -0.4 2,103,382 - wbbI b2034 0.30 -0.9 2,103,382 - wbbI b2034 0.30 -0.2 2,103,392 - wbbI b2034 0.31 -1.2 2,103,393 - wbbI b2034 0.31 +0.4 2,103,436 - wbbI b2034 0.35 -1.7 2,103,436 - wbbI b2034 0.35 -1.0 2,103,449 - wbbI b2034 0.36 +0.7 2,103,490 - wbbI b2034 0.40 -0.1 2,103,490 - wbbI b2034 0.40 -0.0
Or see this region's nucleotide sequence