Experiment: LB Aerobic with Ramoplanin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yliE and yliF are separated by 7 nucleotides yliF and yliG are separated by 46 nucleotides
b0833: yliE - conserved inner membrane protein (NCBI), at 872,202 to 874,550
yliE
b0834: yliF - predicted diguanylate cyclase (NCBI), at 874,558 to 875,886
yliF
b0835: yliG - predicted SAM-dependent methyltransferase (NCBI), at 875,933 to 877,258
yliG
Position (kb) 874
875
876 Strain fitness (log2 ratio) -2
-1
0
1
2 at 873.585 kb on + strand, within yliE at 873.607 kb on + strand, within yliE at 873.607 kb on + strand, within yliE at 873.607 kb on + strand, within yliE at 873.607 kb on + strand, within yliE at 873.615 kb on - strand, within yliE at 873.615 kb on - strand, within yliE at 873.616 kb on + strand, within yliE at 873.616 kb on + strand, within yliE at 873.616 kb on + strand, within yliE at 873.624 kb on - strand, within yliE at 873.630 kb on + strand, within yliE at 873.631 kb on + strand, within yliE at 873.631 kb on + strand, within yliE at 873.638 kb on + strand, within yliE at 873.638 kb on + strand, within yliE at 873.638 kb on - strand, within yliE at 873.656 kb on + strand, within yliE at 873.656 kb on + strand, within yliE at 873.672 kb on + strand, within yliE at 873.706 kb on + strand, within yliE at 873.707 kb on + strand, within yliE at 873.717 kb on + strand, within yliE at 873.721 kb on + strand, within yliE at 873.830 kb on + strand, within yliE at 873.838 kb on + strand, within yliE at 873.838 kb on + strand, within yliE at 873.838 kb on + strand, within yliE at 873.838 kb on + strand, within yliE at 873.838 kb on + strand, within yliE at 873.846 kb on - strand, within yliE at 873.846 kb on - strand, within yliE at 873.846 kb on - strand, within yliE at 873.863 kb on + strand, within yliE at 873.881 kb on - strand, within yliE at 873.881 kb on - strand, within yliE at 873.881 kb on - strand, within yliE at 873.883 kb on - strand, within yliE at 873.888 kb on + strand, within yliE at 873.888 kb on + strand, within yliE at 873.895 kb on + strand, within yliE at 873.896 kb on - strand, within yliE at 873.897 kb on + strand, within yliE at 873.903 kb on - strand, within yliE at 873.904 kb on + strand, within yliE at 873.907 kb on + strand, within yliE at 873.915 kb on - strand, within yliE at 873.926 kb on - strand, within yliE at 873.957 kb on - strand, within yliE at 873.957 kb on - strand, within yliE at 874.001 kb on + strand, within yliE at 874.021 kb on + strand, within yliE at 874.021 kb on + strand, within yliE at 874.051 kb on + strand, within yliE at 874.064 kb on + strand, within yliE at 874.064 kb on + strand, within yliE at 874.081 kb on + strand, within yliE at 874.081 kb on + strand, within yliE at 874.112 kb on + strand, within yliE at 874.112 kb on + strand, within yliE at 874.112 kb on + strand, within yliE at 874.151 kb on - strand, within yliE at 874.153 kb on + strand, within yliE at 874.153 kb on + strand, within yliE at 874.153 kb on + strand, within yliE at 874.153 kb on + strand, within yliE at 874.153 kb on - strand, within yliE at 874.154 kb on + strand, within yliE at 874.154 kb on + strand, within yliE at 874.154 kb on + strand, within yliE at 874.155 kb on - strand, within yliE at 874.156 kb on + strand, within yliE at 874.156 kb on + strand, within yliE at 874.159 kb on + strand, within yliE at 874.159 kb on + strand, within yliE at 874.161 kb on - strand, within yliE at 874.161 kb on - strand, within yliE at 874.164 kb on - strand, within yliE at 874.165 kb on + strand, within yliE at 874.179 kb on + strand, within yliE at 874.179 kb on + strand, within yliE at 874.231 kb on + strand, within yliE at 874.231 kb on + strand, within yliE at 874.236 kb on + strand, within yliE at 874.237 kb on + strand, within yliE at 874.241 kb on + strand, within yliE at 874.243 kb on + strand, within yliE at 874.244 kb on + strand, within yliE at 874.244 kb on + strand, within yliE at 874.244 kb on + strand, within yliE at 874.244 kb on + strand, within yliE at 874.245 kb on - strand, within yliE at 874.251 kb on + strand, within yliE at 874.252 kb on - strand, within yliE at 874.252 kb on - strand, within yliE at 874.252 kb on - strand, within yliE at 874.252 kb on - strand, within yliE at 874.252 kb on - strand, within yliE at 874.259 kb on + strand, within yliE at 874.268 kb on + strand, within yliE at 874.278 kb on + strand, within yliE at 874.278 kb on + strand, within yliE at 874.310 kb on + strand, within yliE at 874.310 kb on + strand, within yliE at 874.312 kb on + strand, within yliE at 874.312 kb on + strand, within yliE at 874.312 kb on + strand, within yliE at 874.312 kb on + strand, within yliE at 874.312 kb on + strand, within yliE at 874.312 kb on + strand, within yliE at 874.312 kb on + strand, within yliE at 874.320 kb on - strand at 874.385 kb on - strand at 874.394 kb on + strand at 874.394 kb on + strand at 874.394 kb on + strand at 874.422 kb on + strand at 874.430 kb on - strand at 874.437 kb on + strand at 874.439 kb on - strand at 874.470 kb on + strand at 874.476 kb on + strand at 874.477 kb on - strand at 874.478 kb on - strand at 874.481 kb on + strand at 874.481 kb on + strand at 874.481 kb on + strand at 874.484 kb on - strand at 874.484 kb on - strand at 874.486 kb on + strand at 874.489 kb on - strand at 874.490 kb on + strand at 874.493 kb on + strand at 874.493 kb on + strand at 874.493 kb on + strand at 874.496 kb on - strand at 874.498 kb on - strand at 874.505 kb on + strand at 874.517 kb on - strand at 874.554 kb on + strand at 874.554 kb on + strand at 874.621 kb on - strand at 874.623 kb on + strand at 874.632 kb on - strand at 874.634 kb on + strand at 874.655 kb on + strand at 874.657 kb on + strand at 874.657 kb on + strand at 874.659 kb on + strand at 874.659 kb on + strand at 874.659 kb on + strand at 874.659 kb on + strand at 874.667 kb on + strand at 874.667 kb on + strand at 874.667 kb on + strand at 874.667 kb on + strand at 874.667 kb on + strand at 874.667 kb on + strand at 874.672 kb on + strand at 874.674 kb on - strand at 874.675 kb on - strand at 874.741 kb on + strand, within yliF at 874.758 kb on + strand, within yliF at 874.758 kb on + strand, within yliF at 874.768 kb on - strand, within yliF at 874.776 kb on + strand, within yliF at 874.787 kb on + strand, within yliF at 874.787 kb on + strand, within yliF at 874.798 kb on + strand, within yliF at 874.806 kb on - strand, within yliF at 874.806 kb on - strand, within yliF at 874.836 kb on + strand, within yliF at 874.854 kb on + strand, within yliF at 874.874 kb on + strand, within yliF at 874.875 kb on + strand, within yliF at 874.882 kb on - strand, within yliF at 874.882 kb on - strand, within yliF at 874.906 kb on + strand, within yliF at 874.906 kb on + strand, within yliF at 874.937 kb on - strand, within yliF at 874.981 kb on + strand, within yliF at 874.985 kb on + strand, within yliF at 874.993 kb on - strand, within yliF at 874.994 kb on - strand, within yliF at 874.994 kb on - strand, within yliF at 874.994 kb on - strand, within yliF at 875.006 kb on + strand, within yliF at 875.029 kb on + strand, within yliF at 875.064 kb on + strand, within yliF at 875.070 kb on + strand, within yliF at 875.095 kb on + strand, within yliF at 875.101 kb on - strand, within yliF at 875.114 kb on - strand, within yliF at 875.198 kb on + strand, within yliF at 875.198 kb on + strand, within yliF at 875.278 kb on + strand, within yliF at 875.278 kb on + strand, within yliF at 875.286 kb on + strand, within yliF at 875.287 kb on + strand, within yliF at 875.292 kb on - strand, within yliF at 875.298 kb on + strand, within yliF at 875.299 kb on - strand, within yliF at 875.300 kb on + strand, within yliF at 875.303 kb on + strand, within yliF at 875.309 kb on + strand, within yliF at 875.320 kb on - strand, within yliF at 875.382 kb on - strand, within yliF at 875.426 kb on + strand, within yliF at 875.430 kb on + strand, within yliF at 875.443 kb on + strand, within yliF at 875.446 kb on + strand, within yliF at 875.451 kb on - strand, within yliF at 875.451 kb on - strand, within yliF at 875.451 kb on - strand, within yliF at 875.457 kb on + strand, within yliF at 875.465 kb on - strand, within yliF at 875.465 kb on - strand, within yliF at 875.495 kb on + strand, within yliF at 875.530 kb on + strand, within yliF at 875.566 kb on + strand, within yliF at 875.573 kb on + strand, within yliF at 875.593 kb on + strand, within yliF at 875.596 kb on - strand, within yliF at 875.643 kb on + strand, within yliF at 875.643 kb on + strand, within yliF at 875.643 kb on - strand, within yliF at 875.645 kb on + strand, within yliF at 875.651 kb on - strand, within yliF at 875.651 kb on - strand, within yliF at 875.651 kb on - strand, within yliF at 875.658 kb on + strand, within yliF at 875.658 kb on + strand, within yliF at 875.699 kb on + strand, within yliF at 875.701 kb on + strand, within yliF at 875.701 kb on + strand, within yliF at 875.701 kb on + strand, within yliF at 875.707 kb on - strand, within yliF at 875.717 kb on - strand, within yliF at 875.785 kb on - strand at 875.798 kb on + strand at 875.799 kb on - strand at 875.807 kb on - strand at 875.807 kb on - strand at 875.811 kb on - strand at 875.811 kb on - strand at 875.838 kb on + strand at 875.838 kb on + strand at 875.839 kb on + strand at 875.839 kb on + strand at 875.847 kb on - strand at 875.849 kb on - strand at 875.855 kb on - strand at 875.855 kb on - strand at 875.855 kb on - strand at 875.871 kb on + strand at 875.879 kb on - strand at 875.985 kb on - strand at 875.997 kb on - strand at 875.997 kb on - strand at 876.196 kb on - strand, within yliG at 876.369 kb on - strand, within yliG at 876.423 kb on - strand, within yliG at 876.439 kb on - strand, within yliG at 876.439 kb on - strand, within yliG at 876.546 kb on + strand, within yliG at 876.546 kb on + strand, within yliG at 876.551 kb on + strand, within yliG at 876.584 kb on - strand, within yliG at 876.629 kb on + strand, within yliG at 876.713 kb on + strand, within yliG at 876.716 kb on - strand, within yliG at 876.729 kb on + strand, within yliG at 876.729 kb on + strand, within yliG at 876.763 kb on - strand, within yliG at 876.766 kb on - strand, within yliG at 876.845 kb on - strand, within yliG at 876.877 kb on + strand, within yliG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Ramoplanin 0.000125 mM remove 873,585 + yliE b0833 0.59 +0.5 873,607 + yliE b0833 0.60 -1.1 873,607 + yliE b0833 0.60 -0.7 873,607 + yliE b0833 0.60 -0.1 873,607 + yliE b0833 0.60 +1.1 873,615 - yliE b0833 0.60 +1.5 873,615 - yliE b0833 0.60 +1.5 873,616 + yliE b0833 0.60 +0.2 873,616 + yliE b0833 0.60 -1.2 873,616 + yliE b0833 0.60 +0.8 873,624 - yliE b0833 0.61 +0.5 873,630 + yliE b0833 0.61 -1.8 873,631 + yliE b0833 0.61 +0.5 873,631 + yliE b0833 0.61 -0.4 873,638 + yliE b0833 0.61 +1.8 873,638 + yliE b0833 0.61 -0.7 873,638 - yliE b0833 0.61 +1.5 873,656 + yliE b0833 0.62 -1.6 873,656 + yliE b0833 0.62 -0.5 873,672 + yliE b0833 0.63 -0.9 873,706 + yliE b0833 0.64 -0.8 873,707 + yliE b0833 0.64 -0.9 873,717 + yliE b0833 0.64 +0.9 873,721 + yliE b0833 0.65 -0.3 873,830 + yliE b0833 0.69 +0.9 873,838 + yliE b0833 0.70 -0.5 873,838 + yliE b0833 0.70 -0.1 873,838 + yliE b0833 0.70 +1.1 873,838 + yliE b0833 0.70 -1.1 873,838 + yliE b0833 0.70 +1.3 873,846 - yliE b0833 0.70 +0.5 873,846 - yliE b0833 0.70 +0.5 873,846 - yliE b0833 0.70 +1.5 873,863 + yliE b0833 0.71 +0.2 873,881 - yliE b0833 0.71 +1.5 873,881 - yliE b0833 0.71 +1.5 873,881 - yliE b0833 0.71 -0.8 873,883 - yliE b0833 0.72 -0.1 873,888 + yliE b0833 0.72 -0.1 873,888 + yliE b0833 0.72 +1.5 873,895 + yliE b0833 0.72 -1.4 873,896 - yliE b0833 0.72 -2.4 873,897 + yliE b0833 0.72 -0.3 873,903 - yliE b0833 0.72 +0.3 873,904 + yliE b0833 0.72 -0.1 873,907 + yliE b0833 0.73 +0.7 873,915 - yliE b0833 0.73 -0.5 873,926 - yliE b0833 0.73 +0.5 873,957 - yliE b0833 0.75 +1.5 873,957 - yliE b0833 0.75 +1.5 874,001 + yliE b0833 0.77 +1.5 874,021 + yliE b0833 0.77 -1.9 874,021 + yliE b0833 0.77 +0.1 874,051 + yliE b0833 0.79 -2.2 874,064 + yliE b0833 0.79 +1.5 874,064 + yliE b0833 0.79 +0.9 874,081 + yliE b0833 0.80 +1.5 874,081 + yliE b0833 0.80 +0.5 874,112 + yliE b0833 0.81 +1.1 874,112 + yliE b0833 0.81 -1.1 874,112 + yliE b0833 0.81 -0.3 874,151 - yliE b0833 0.83 +0.8 874,153 + yliE b0833 0.83 -1.0 874,153 + yliE b0833 0.83 +1.5 874,153 + yliE b0833 0.83 -0.8 874,153 + yliE b0833 0.83 -1.1 874,153 - yliE b0833 0.83 +1.5 874,154 + yliE b0833 0.83 -0.5 874,154 + yliE b0833 0.83 +0.2 874,154 + yliE b0833 0.83 +0.5 874,155 - yliE b0833 0.83 +1.5 874,156 + yliE b0833 0.83 -0.2 874,156 + yliE b0833 0.83 +0.9 874,159 + yliE b0833 0.83 -1.3 874,159 + yliE b0833 0.83 +0.8 874,161 - yliE b0833 0.83 +0.5 874,161 - yliE b0833 0.83 +0.5 874,164 - yliE b0833 0.84 +1.5 874,165 + yliE b0833 0.84 -0.8 874,179 + yliE b0833 0.84 -0.2 874,179 + yliE b0833 0.84 +0.5 874,231 + yliE b0833 0.86 +1.5 874,231 + yliE b0833 0.86 -0.5 874,236 + yliE b0833 0.87 -1.3 874,237 + yliE b0833 0.87 +0.5 874,241 + yliE b0833 0.87 -0.5 874,243 + yliE b0833 0.87 +0.5 874,244 + yliE b0833 0.87 -0.8 874,244 + yliE b0833 0.87 -1.3 874,244 + yliE b0833 0.87 +0.5 874,244 + yliE b0833 0.87 -1.3 874,245 - yliE b0833 0.87 -0.3 874,251 + yliE b0833 0.87 +1.5 874,252 - yliE b0833 0.87 -0.1 874,252 - yliE b0833 0.87 +1.9 874,252 - yliE b0833 0.87 +0.5 874,252 - yliE b0833 0.87 +0.5 874,252 - yliE b0833 0.87 -0.6 874,259 + yliE b0833 0.88 -0.8 874,268 + yliE b0833 0.88 -1.9 874,278 + yliE b0833 0.88 -0.2 874,278 + yliE b0833 0.88 -1.3 874,310 + yliE b0833 0.90 -1.4 874,310 + yliE b0833 0.90 +0.5 874,312 + yliE b0833 0.90 -0.9 874,312 + yliE b0833 0.90 +1.0 874,312 + yliE b0833 0.90 +1.0 874,312 + yliE b0833 0.90 -0.5 874,312 + yliE b0833 0.90 -1.6 874,312 + yliE b0833 0.90 -1.4 874,312 + yliE b0833 0.90 +0.5 874,320 - +1.5 874,385 - +1.5 874,394 + +1.5 874,394 + +1.5 874,394 + -0.0 874,422 + -1.0 874,430 - +0.5 874,437 + -0.8 874,439 - +1.1 874,470 + +0.4 874,476 + +1.2 874,477 - +0.5 874,478 - +0.7 874,481 + -0.0 874,481 + -0.2 874,481 + -0.0 874,484 - -0.0 874,484 - +0.5 874,486 + -0.5 874,489 - -1.0 874,490 + -0.9 874,493 + -0.5 874,493 + -0.8 874,493 + +1.5 874,496 - +0.5 874,498 - +1.5 874,505 + +0.3 874,517 - +1.5 874,554 + +1.5 874,554 + -0.5 874,621 - +1.1 874,623 + +0.5 874,632 - -0.6 874,634 + -1.5 874,655 + +0.6 874,657 + -0.3 874,657 + -1.5 874,659 + -0.0 874,659 + +0.7 874,659 + -0.5 874,659 + -0.2 874,667 + -0.4 874,667 + -0.5 874,667 + +0.2 874,667 + -1.0 874,667 + -0.1 874,667 + -0.1 874,672 + -1.3 874,674 - +0.5 874,675 - +1.5 874,741 + yliF b0834 0.14 +0.5 874,758 + yliF b0834 0.15 -1.0 874,758 + yliF b0834 0.15 +1.5 874,768 - yliF b0834 0.16 +1.5 874,776 + yliF b0834 0.16 +0.2 874,787 + yliF b0834 0.17 +0.5 874,787 + yliF b0834 0.17 +1.5 874,798 + yliF b0834 0.18 -0.9 874,806 - yliF b0834 0.19 +1.5 874,806 - yliF b0834 0.19 +1.5 874,836 + yliF b0834 0.21 -1.5 874,854 + yliF b0834 0.22 -0.4 874,874 + yliF b0834 0.24 +0.5 874,875 + yliF b0834 0.24 -1.9 874,882 - yliF b0834 0.24 -0.0 874,882 - yliF b0834 0.24 +1.5 874,906 + yliF b0834 0.26 -0.5 874,906 + yliF b0834 0.26 +2.7 874,937 - yliF b0834 0.29 -1.3 874,981 + yliF b0834 0.32 -0.0 874,985 + yliF b0834 0.32 +0.5 874,993 - yliF b0834 0.33 -2.0 874,994 - yliF b0834 0.33 -0.0 874,994 - yliF b0834 0.33 +0.3 874,994 - yliF b0834 0.33 -0.0 875,006 + yliF b0834 0.34 -0.0 875,029 + yliF b0834 0.35 -1.5 875,064 + yliF b0834 0.38 -0.0 875,070 + yliF b0834 0.39 -1.3 875,095 + yliF b0834 0.40 -0.5 875,101 - yliF b0834 0.41 +1.5 875,114 - yliF b0834 0.42 +0.5 875,198 + yliF b0834 0.48 +1.4 875,198 + yliF b0834 0.48 -0.2 875,278 + yliF b0834 0.54 +1.5 875,278 + yliF b0834 0.54 +0.5 875,286 + yliF b0834 0.55 -0.5 875,287 + yliF b0834 0.55 +0.5 875,292 - yliF b0834 0.55 +0.5 875,298 + yliF b0834 0.56 +0.5 875,299 - yliF b0834 0.56 -0.5 875,300 + yliF b0834 0.56 +1.5 875,303 + yliF b0834 0.56 +0.5 875,309 + yliF b0834 0.57 +0.5 875,320 - yliF b0834 0.57 +1.5 875,382 - yliF b0834 0.62 -0.0 875,426 + yliF b0834 0.65 -1.3 875,430 + yliF b0834 0.66 +0.5 875,443 + yliF b0834 0.67 -0.0 875,446 + yliF b0834 0.67 +1.0 875,451 - yliF b0834 0.67 +0.5 875,451 - yliF b0834 0.67 +1.5 875,451 - yliF b0834 0.67 -1.3 875,457 + yliF b0834 0.68 -0.5 875,465 - yliF b0834 0.68 -0.0 875,465 - yliF b0834 0.68 -0.8 875,495 + yliF b0834 0.71 +1.1 875,530 + yliF b0834 0.73 -2.4 875,566 + yliF b0834 0.76 -1.0 875,573 + yliF b0834 0.76 -0.9 875,593 + yliF b0834 0.78 -1.5 875,596 - yliF b0834 0.78 -0.5 875,643 + yliF b0834 0.82 -0.0 875,643 + yliF b0834 0.82 +0.7 875,643 - yliF b0834 0.82 +1.5 875,645 + yliF b0834 0.82 +2.0 875,651 - yliF b0834 0.82 +0.5 875,651 - yliF b0834 0.82 -0.0 875,651 - yliF b0834 0.82 +0.8 875,658 + yliF b0834 0.83 -1.5 875,658 + yliF b0834 0.83 -1.3 875,699 + yliF b0834 0.86 +2.1 875,701 + yliF b0834 0.86 -0.5 875,701 + yliF b0834 0.86 -0.2 875,701 + yliF b0834 0.86 +2.1 875,707 - yliF b0834 0.86 +0.5 875,717 - yliF b0834 0.87 +0.5 875,785 - +1.5 875,798 + +0.5 875,799 - -1.0 875,807 - -1.8 875,807 - -0.0 875,811 - +2.1 875,811 - +1.5 875,838 + +1.0 875,838 + -0.8 875,839 + -0.8 875,839 + -0.9 875,847 - -1.9 875,849 - +1.0 875,855 - -1.6 875,855 - +1.8 875,855 - +1.9 875,871 + -0.7 875,879 - +1.8 875,985 - -1.0 875,997 - +2.1 875,997 - +1.2 876,196 - yliG b0835 0.20 +0.3 876,369 - yliG b0835 0.33 -0.6 876,423 - yliG b0835 0.37 -0.5 876,439 - yliG b0835 0.38 -0.8 876,439 - yliG b0835 0.38 +1.7 876,546 + yliG b0835 0.46 +0.1 876,546 + yliG b0835 0.46 -0.5 876,551 + yliG b0835 0.47 -0.5 876,584 - yliG b0835 0.49 -1.8 876,629 + yliG b0835 0.52 +1.0 876,713 + yliG b0835 0.59 +0.1 876,716 - yliG b0835 0.59 +0.2 876,729 + yliG b0835 0.60 +0.9 876,729 + yliG b0835 0.60 +1.1 876,763 - yliG b0835 0.63 +0.6 876,766 - yliG b0835 0.63 +0.8 876,845 - yliG b0835 0.69 +1.3 876,877 + yliG b0835 0.71 -1.3
Or see this region's nucleotide sequence