Experiment: LB Aerobic with Ramoplanin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt caiE and caiD are separated by 5 nucleotides caiD and caiC are separated by 108 nucleotides caiC and caiB are separated by 73 nucleotides
b0035: caiE - possible synthesis of cofactor for carnitine racemase and dehydratase (VIMSS), at 34,781 to 35,371
caiE
b0036: caiD - crotonobetainyl CoA hydratase (RefSeq), at 35,377 to 36,162
caiD
b0037: caiC - predicted crotonobetaine CoA ligase:carnitine CoA ligase (RefSeq), at 36,271 to 37,824
caiC
b0038: caiB - crotonobetainyl-CoA:carnitineCoA-transferase (NCBI), at 37,898 to 39,115
caiB
Position (kb) 36
37
38 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 35.272 kb on - strand, within caiE at 35.312 kb on - strand, within caiE at 35.312 kb on - strand, within caiE at 35.312 kb on - strand, within caiE at 35.349 kb on - strand at 35.428 kb on + strand at 35.467 kb on - strand, within caiD at 35.501 kb on + strand, within caiD at 35.618 kb on - strand, within caiD at 35.640 kb on - strand, within caiD at 35.731 kb on + strand, within caiD at 35.743 kb on + strand, within caiD at 35.857 kb on + strand, within caiD at 35.907 kb on - strand, within caiD at 35.924 kb on - strand, within caiD at 35.924 kb on - strand, within caiD at 35.935 kb on - strand, within caiD at 35.937 kb on + strand, within caiD at 35.937 kb on + strand, within caiD at 35.940 kb on + strand, within caiD at 35.941 kb on - strand, within caiD at 35.963 kb on + strand, within caiD at 35.989 kb on + strand, within caiD at 35.989 kb on - strand, within caiD at 35.989 kb on - strand, within caiD at 35.994 kb on + strand, within caiD at 35.994 kb on - strand, within caiD at 36.004 kb on + strand, within caiD at 36.014 kb on - strand, within caiD at 36.046 kb on + strand, within caiD at 36.052 kb on + strand, within caiD at 36.089 kb on - strand at 36.104 kb on - strand at 36.139 kb on + strand at 36.139 kb on + strand at 36.147 kb on - strand at 36.150 kb on + strand at 36.158 kb on - strand at 36.191 kb on + strand at 36.191 kb on + strand at 36.203 kb on - strand at 36.203 kb on - strand at 36.249 kb on + strand at 36.254 kb on + strand at 36.257 kb on - strand at 36.326 kb on - strand at 36.330 kb on + strand at 36.339 kb on - strand at 36.339 kb on - strand at 36.344 kb on - strand at 36.405 kb on - strand at 36.405 kb on - strand at 36.405 kb on - strand at 36.406 kb on - strand at 36.410 kb on + strand at 36.418 kb on - strand at 36.418 kb on - strand at 36.423 kb on - strand at 36.428 kb on - strand, within caiC at 36.431 kb on + strand, within caiC at 36.441 kb on + strand, within caiC at 36.453 kb on - strand, within caiC at 36.456 kb on + strand, within caiC at 36.460 kb on - strand, within caiC at 36.471 kb on + strand, within caiC at 36.471 kb on + strand, within caiC at 36.522 kb on - strand, within caiC at 36.522 kb on - strand, within caiC at 36.530 kb on - strand, within caiC at 36.530 kb on - strand, within caiC at 36.532 kb on - strand, within caiC at 36.532 kb on - strand, within caiC at 36.542 kb on - strand, within caiC at 36.542 kb on - strand, within caiC at 36.555 kb on - strand, within caiC at 36.565 kb on - strand, within caiC at 36.565 kb on - strand, within caiC at 36.576 kb on - strand, within caiC at 36.576 kb on - strand, within caiC at 36.609 kb on - strand, within caiC at 36.612 kb on - strand, within caiC at 36.612 kb on - strand, within caiC at 36.620 kb on + strand, within caiC at 36.628 kb on - strand, within caiC at 36.628 kb on - strand, within caiC at 36.666 kb on - strand, within caiC at 36.673 kb on - strand, within caiC at 36.759 kb on - strand, within caiC at 36.759 kb on - strand, within caiC at 36.888 kb on + strand, within caiC at 36.888 kb on + strand, within caiC at 36.924 kb on + strand, within caiC at 37.130 kb on + strand, within caiC at 37.155 kb on + strand, within caiC at 37.196 kb on + strand, within caiC at 37.219 kb on - strand, within caiC at 37.338 kb on - strand, within caiC at 37.460 kb on - strand, within caiC at 37.460 kb on - strand, within caiC at 37.463 kb on + strand, within caiC at 37.463 kb on + strand, within caiC at 37.480 kb on + strand, within caiC at 37.497 kb on + strand, within caiC at 37.534 kb on - strand, within caiC at 37.612 kb on + strand, within caiC at 37.623 kb on + strand, within caiC at 37.623 kb on + strand, within caiC at 37.634 kb on - strand, within caiC at 37.634 kb on - strand, within caiC at 37.985 kb on + strand at 37.993 kb on - strand at 37.993 kb on - strand at 38.049 kb on + strand, within caiB at 38.059 kb on - strand, within caiB at 38.098 kb on - strand, within caiB at 38.109 kb on + strand, within caiB at 38.143 kb on - strand, within caiB at 38.144 kb on + strand, within caiB at 38.144 kb on + strand, within caiB at 38.197 kb on - strand, within caiB at 38.197 kb on - strand, within caiB at 38.242 kb on + strand, within caiB at 38.279 kb on - strand, within caiB at 38.279 kb on - strand, within caiB at 38.286 kb on - strand, within caiB at 38.314 kb on + strand, within caiB at 38.325 kb on - strand, within caiB at 38.340 kb on - strand, within caiB at 38.361 kb on - strand, within caiB at 38.361 kb on - strand, within caiB at 38.368 kb on + strand, within caiB at 38.368 kb on - strand, within caiB at 38.372 kb on + strand, within caiB at 38.394 kb on + strand, within caiB at 38.428 kb on - strand, within caiB at 38.482 kb on - strand, within caiB at 38.494 kb on - strand, within caiB at 38.516 kb on - strand, within caiB at 38.564 kb on - strand, within caiB at 38.599 kb on - strand, within caiB at 38.699 kb on - strand, within caiB at 38.724 kb on + strand, within caiB at 38.732 kb on - strand, within caiB at 38.732 kb on - strand, within caiB at 38.771 kb on + strand, within caiB at 38.771 kb on + strand, within caiB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Ramoplanin 0.000125 mM remove 35,272 - caiE b0035 0.83 +0.6 35,312 - caiE b0035 0.90 +0.4 35,312 - caiE b0035 0.90 -2.2 35,312 - caiE b0035 0.90 +2.1 35,349 - -0.8 35,428 + -0.7 35,467 - caiD b0036 0.11 -0.6 35,501 + caiD b0036 0.16 -0.8 35,618 - caiD b0036 0.31 -0.2 35,640 - caiD b0036 0.33 -0.1 35,731 + caiD b0036 0.45 +1.7 35,743 + caiD b0036 0.47 -0.9 35,857 + caiD b0036 0.61 -1.8 35,907 - caiD b0036 0.67 -0.2 35,924 - caiD b0036 0.70 +0.5 35,924 - caiD b0036 0.70 -1.5 35,935 - caiD b0036 0.71 -2.1 35,937 + caiD b0036 0.71 +0.8 35,937 + caiD b0036 0.71 -0.8 35,940 + caiD b0036 0.72 +0.2 35,941 - caiD b0036 0.72 +1.0 35,963 + caiD b0036 0.75 -0.1 35,989 + caiD b0036 0.78 -2.4 35,989 - caiD b0036 0.78 +0.5 35,989 - caiD b0036 0.78 +1.1 35,994 + caiD b0036 0.78 +1.8 35,994 - caiD b0036 0.78 -1.6 36,004 + caiD b0036 0.80 +0.9 36,014 - caiD b0036 0.81 +1.3 36,046 + caiD b0036 0.85 -0.6 36,052 + caiD b0036 0.86 -0.4 36,089 - -1.2 36,104 - -0.2 36,139 + -0.1 36,139 + +1.0 36,147 - -2.2 36,150 + +0.8 36,158 - -0.1 36,191 + +1.8 36,191 + -1.4 36,203 - +0.6 36,203 - +0.6 36,249 + +1.8 36,254 + +1.8 36,257 - +0.2 36,326 - -2.4 36,330 + +0.2 36,339 - -0.1 36,339 - -2.4 36,344 - -0.3 36,405 - -1.8 36,405 - +1.1 36,405 - -0.4 36,406 - -2.4 36,410 + -0.2 36,418 - +0.2 36,418 - +0.1 36,423 - -1.2 36,428 - caiC b0037 0.10 -0.1 36,431 + caiC b0037 0.10 -1.6 36,441 + caiC b0037 0.11 -0.8 36,453 - caiC b0037 0.12 +0.2 36,456 + caiC b0037 0.12 -1.8 36,460 - caiC b0037 0.12 -0.6 36,471 + caiC b0037 0.13 -0.6 36,471 + caiC b0037 0.13 -0.2 36,522 - caiC b0037 0.16 -1.1 36,522 - caiC b0037 0.16 +0.5 36,530 - caiC b0037 0.17 -1.3 36,530 - caiC b0037 0.17 -0.0 36,532 - caiC b0037 0.17 -0.8 36,532 - caiC b0037 0.17 -0.2 36,542 - caiC b0037 0.17 -0.2 36,542 - caiC b0037 0.17 +0.1 36,555 - caiC b0037 0.18 -0.6 36,565 - caiC b0037 0.19 +0.2 36,565 - caiC b0037 0.19 -0.5 36,576 - caiC b0037 0.20 -0.7 36,576 - caiC b0037 0.20 +0.0 36,609 - caiC b0037 0.22 -0.3 36,612 - caiC b0037 0.22 -1.2 36,612 - caiC b0037 0.22 +0.6 36,620 + caiC b0037 0.22 -1.6 36,628 - caiC b0037 0.23 -0.7 36,628 - caiC b0037 0.23 -0.2 36,666 - caiC b0037 0.25 +0.3 36,673 - caiC b0037 0.26 -1.7 36,759 - caiC b0037 0.31 -0.7 36,759 - caiC b0037 0.31 -0.3 36,888 + caiC b0037 0.40 +2.9 36,888 + caiC b0037 0.40 +1.6 36,924 + caiC b0037 0.42 +0.7 37,130 + caiC b0037 0.55 -0.4 37,155 + caiC b0037 0.57 +0.3 37,196 + caiC b0037 0.60 +0.2 37,219 - caiC b0037 0.61 -0.9 37,338 - caiC b0037 0.69 -0.1 37,460 - caiC b0037 0.77 +0.2 37,460 - caiC b0037 0.77 -1.6 37,463 + caiC b0037 0.77 -2.5 37,463 + caiC b0037 0.77 +0.8 37,480 + caiC b0037 0.78 +0.0 37,497 + caiC b0037 0.79 +0.0 37,534 - caiC b0037 0.81 -0.2 37,612 + caiC b0037 0.86 -0.5 37,623 + caiC b0037 0.87 +1.8 37,623 + caiC b0037 0.87 -2.3 37,634 - caiC b0037 0.88 -0.1 37,634 - caiC b0037 0.88 -0.9 37,985 + -0.2 37,993 - -0.7 37,993 - +0.6 38,049 + caiB b0038 0.12 -1.4 38,059 - caiB b0038 0.13 +0.5 38,098 - caiB b0038 0.16 +0.1 38,109 + caiB b0038 0.17 -0.7 38,143 - caiB b0038 0.20 -0.8 38,144 + caiB b0038 0.20 +0.2 38,144 + caiB b0038 0.20 -0.4 38,197 - caiB b0038 0.25 +0.3 38,197 - caiB b0038 0.25 +0.3 38,242 + caiB b0038 0.28 -0.7 38,279 - caiB b0038 0.31 -0.1 38,279 - caiB b0038 0.31 +0.5 38,286 - caiB b0038 0.32 +0.5 38,314 + caiB b0038 0.34 +0.2 38,325 - caiB b0038 0.35 +1.8 38,340 - caiB b0038 0.36 +0.4 38,361 - caiB b0038 0.38 +0.1 38,361 - caiB b0038 0.38 +0.3 38,368 + caiB b0038 0.39 -0.1 38,368 - caiB b0038 0.39 +0.3 38,372 + caiB b0038 0.39 +0.0 38,394 + caiB b0038 0.41 +0.9 38,428 - caiB b0038 0.44 +0.1 38,482 - caiB b0038 0.48 -1.0 38,494 - caiB b0038 0.49 -0.0 38,516 - caiB b0038 0.51 -0.4 38,564 - caiB b0038 0.55 -0.6 38,599 - caiB b0038 0.58 -0.1 38,699 - caiB b0038 0.66 -0.7 38,724 + caiB b0038 0.68 -0.6 38,732 - caiB b0038 0.68 -0.6 38,732 - caiB b0038 0.68 +0.3 38,771 + caiB b0038 0.72 +0.7 38,771 + caiB b0038 0.72 -1.8
Or see this region's nucleotide sequence