Experiment: KB with Novobiocin 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4634 and Psyr_4635 are separated by 98 nucleotides Psyr_4635 and Psyr_4636 overlap by 10 nucleotides Psyr_4636 and Psyr_4637 are separated by 65 nucleotides
Psyr_4634: Psyr_4634 - Polynucleotide adenylyltransferase:Metal-dependent phosphohydrolase, HD subdomain protein, at 5,495,417 to 5,496,643
_4634
Psyr_4635: Psyr_4635 - 7, 8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPK, at 5,496,742 to 5,497,266
_4635
Psyr_4636: Psyr_4636 - Dihydroneopterin aldolase family:Dihydroneopterin aldolase, at 5,497,257 to 5,497,610
_4636
Psyr_4637: Psyr_4637 - acyl-phosphate glycerol-3-phosphate acyltransferase, at 5,497,676 to 5,498,254
_4637
Position (kb) 5496
5497
5498 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5496.093 kb on + strand, within Psyr_4634 at 5496.093 kb on + strand, within Psyr_4634 at 5496.623 kb on + strand at 5496.623 kb on + strand at 5496.688 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.693 kb on - strand at 5496.693 kb on - strand at 5496.748 kb on - strand at 5496.905 kb on - strand, within Psyr_4635 at 5496.905 kb on - strand, within Psyr_4635 at 5497.652 kb on - strand at 5497.970 kb on + strand, within Psyr_4637 at 5497.970 kb on + strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5498.055 kb on + strand, within Psyr_4637 at 5498.217 kb on + strand at 5498.218 kb on - strand at 5498.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 7.75 ug/ml remove 5,496,093 + Psyr_4634 0.55 -1.0 5,496,093 + Psyr_4634 0.55 +1.2 5,496,623 + +0.4 5,496,623 + -1.0 5,496,688 + -0.7 5,496,692 + -4.0 5,496,692 + +0.2 5,496,692 + -1.4 5,496,692 + -0.4 5,496,692 + -0.0 5,496,692 + -0.3 5,496,692 + -0.6 5,496,692 + -1.2 5,496,693 - -0.4 5,496,693 - -0.5 5,496,748 - -0.4 5,496,905 - Psyr_4635 0.31 -2.9 5,496,905 - Psyr_4635 0.31 +1.7 5,497,652 - -0.6 5,497,970 + Psyr_4637 0.51 +0.4 5,497,970 + Psyr_4637 0.51 +0.1 5,497,971 - Psyr_4637 0.51 +0.7 5,497,971 - Psyr_4637 0.51 +0.2 5,497,971 - Psyr_4637 0.51 +0.3 5,498,055 + Psyr_4637 0.65 +2.9 5,498,217 + -0.6 5,498,218 - +0.3 5,498,218 - +2.5
Or see this region's nucleotide sequence