Experiment: KB with Novobiocin 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3255 and Psyr_3256 overlap by 4 nucleotides Psyr_3256 and Psyr_3257 overlap by 4 nucleotides Psyr_3257 and Psyr_3258 are separated by 18 nucleotides Psyr_3258 and Psyr_3259 are separated by 443 nucleotides Psyr_3259 and Psyr_3260 are separated by 19 nucleotides
Psyr_3255: Psyr_3255 - multisubunit potassium/proton antiporter, PhaE subunit, at 3,900,749 to 3,901,237
_3255
Psyr_3256: Psyr_3256 - multisubunit potassium/proton antiporter, PhaF subunit, at 3,901,234 to 3,901,512
_3256
Psyr_3257: Psyr_3257 - multisubunit potassium/proton antiporter, PhaG subunit, at 3,901,509 to 3,901,889
_3257
Psyr_3258: Psyr_3258 - conserved hypothetical protein, at 3,901,908 to 3,902,375
_3258
Psyr_3259: Psyr_3259 - conserved hypothetical protein, at 3,902,819 to 3,903,058
_3259
Psyr_3260: Psyr_3260 - conserved domain protein, at 3,903,078 to 3,904,562
_3260
Position (kb)
3901
3902
3903 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3900.908 kb on - strand, within Psyr_3255 at 3900.940 kb on - strand, within Psyr_3255 at 3901.205 kb on + strand at 3901.205 kb on + strand at 3901.205 kb on + strand at 3901.205 kb on + strand at 3901.364 kb on - strand, within Psyr_3256 at 3901.364 kb on - strand, within Psyr_3256 at 3901.369 kb on - strand, within Psyr_3256 at 3901.369 kb on - strand, within Psyr_3256 at 3901.420 kb on + strand, within Psyr_3256 at 3901.420 kb on + strand, within Psyr_3256 at 3901.420 kb on + strand, within Psyr_3256 at 3901.421 kb on - strand, within Psyr_3256 at 3901.421 kb on - strand, within Psyr_3256 at 3901.526 kb on + strand at 3901.526 kb on + strand at 3901.526 kb on + strand at 3901.526 kb on + strand at 3901.549 kb on + strand, within Psyr_3257 at 3901.701 kb on + strand, within Psyr_3257 at 3901.701 kb on + strand, within Psyr_3257 at 3901.701 kb on + strand, within Psyr_3257 at 3901.702 kb on - strand, within Psyr_3257 at 3901.702 kb on - strand, within Psyr_3257 at 3901.702 kb on - strand, within Psyr_3257 at 3901.910 kb on - strand at 3902.194 kb on - strand, within Psyr_3258 at 3902.254 kb on + strand, within Psyr_3258 at 3902.254 kb on + strand, within Psyr_3258 at 3902.254 kb on + strand, within Psyr_3258 at 3902.254 kb on + strand, within Psyr_3258 at 3902.254 kb on + strand, within Psyr_3258 at 3902.254 kb on + strand, within Psyr_3258 at 3902.255 kb on - strand, within Psyr_3258 at 3902.255 kb on - strand, within Psyr_3258 at 3902.255 kb on - strand, within Psyr_3258 at 3902.363 kb on + strand at 3902.363 kb on + strand at 3902.364 kb on - strand at 3902.367 kb on + strand at 3902.378 kb on + strand at 3902.378 kb on + strand at 3902.379 kb on - strand at 3902.443 kb on + strand at 3902.563 kb on - strand at 3902.564 kb on + strand at 3902.564 kb on + strand at 3902.564 kb on + strand at 3902.564 kb on + strand at 3902.565 kb on - strand at 3902.565 kb on - strand at 3902.565 kb on - strand at 3902.565 kb on - strand at 3902.565 kb on - strand at 3902.691 kb on + strand at 3902.691 kb on + strand at 3902.691 kb on + strand at 3902.691 kb on + strand at 3902.692 kb on - strand at 3902.692 kb on - strand at 3902.698 kb on + strand at 3902.698 kb on + strand at 3902.698 kb on + strand at 3902.699 kb on - strand at 3902.699 kb on - strand at 3902.699 kb on - strand at 3902.699 kb on - strand at 3902.699 kb on - strand at 3902.699 kb on - strand at 3902.699 kb on - strand at 3902.775 kb on + strand at 3902.798 kb on - strand at 3902.830 kb on + strand at 3902.830 kb on + strand at 3902.831 kb on - strand at 3902.831 kb on - strand at 3902.831 kb on - strand at 3902.836 kb on + strand at 3902.836 kb on + strand at 3902.837 kb on - strand at 3903.184 kb on - strand at 3903.184 kb on - strand at 3903.184 kb on - strand at 3903.184 kb on - strand at 3903.214 kb on + strand at 3903.214 kb on + strand at 3903.215 kb on - strand at 3903.215 kb on - strand at 3903.228 kb on + strand, within Psyr_3260 at 3903.229 kb on - strand, within Psyr_3260 at 3903.229 kb on - strand, within Psyr_3260
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 7.75 ug/ml remove 3,900,908 - Psyr_3255 0.33 +0.1 3,900,940 - Psyr_3255 0.39 +0.3 3,901,205 + +0.5 3,901,205 + -3.5 3,901,205 + -0.9 3,901,205 + +0.7 3,901,364 - Psyr_3256 0.47 -0.3 3,901,364 - Psyr_3256 0.47 +0.2 3,901,369 - Psyr_3256 0.48 +1.6 3,901,369 - Psyr_3256 0.48 -0.8 3,901,420 + Psyr_3256 0.67 -0.3 3,901,420 + Psyr_3256 0.67 +0.7 3,901,420 + Psyr_3256 0.67 +2.7 3,901,421 - Psyr_3256 0.67 -1.3 3,901,421 - Psyr_3256 0.67 +0.2 3,901,526 + -0.2 3,901,526 + -0.8 3,901,526 + +1.0 3,901,526 + -0.8 3,901,549 + Psyr_3257 0.10 -0.2 3,901,701 + Psyr_3257 0.50 -2.6 3,901,701 + Psyr_3257 0.50 +0.1 3,901,701 + Psyr_3257 0.50 -0.4 3,901,702 - Psyr_3257 0.51 +1.4 3,901,702 - Psyr_3257 0.51 -1.4 3,901,702 - Psyr_3257 0.51 +0.8 3,901,910 - +0.5 3,902,194 - Psyr_3258 0.61 +1.6 3,902,254 + Psyr_3258 0.74 -3.6 3,902,254 + Psyr_3258 0.74 -0.5 3,902,254 + Psyr_3258 0.74 -0.6 3,902,254 + Psyr_3258 0.74 +2.1 3,902,254 + Psyr_3258 0.74 -2.0 3,902,254 + Psyr_3258 0.74 -2.6 3,902,255 - Psyr_3258 0.74 +0.5 3,902,255 - Psyr_3258 0.74 +0.3 3,902,255 - Psyr_3258 0.74 -1.7 3,902,363 + +0.9 3,902,363 + +1.1 3,902,364 - -0.7 3,902,367 + -1.7 3,902,378 + -0.5 3,902,378 + -0.9 3,902,379 - -2.3 3,902,443 + -0.3 3,902,563 - +1.0 3,902,564 + -1.7 3,902,564 + -1.3 3,902,564 + -1.4 3,902,564 + -0.1 3,902,565 - +0.2 3,902,565 - +0.1 3,902,565 - +0.2 3,902,565 - -1.5 3,902,565 - -0.2 3,902,691 + -0.1 3,902,691 + +0.4 3,902,691 + -2.2 3,902,691 + +0.4 3,902,692 - +0.9 3,902,692 - -0.3 3,902,698 + +0.6 3,902,698 + +2.3 3,902,698 + -0.6 3,902,699 - -1.6 3,902,699 - -0.2 3,902,699 - -0.4 3,902,699 - +0.0 3,902,699 - +0.6 3,902,699 - +1.1 3,902,699 - +0.5 3,902,775 + +0.4 3,902,798 - +0.2 3,902,830 + +0.0 3,902,830 + -0.5 3,902,831 - +2.3 3,902,831 - +0.6 3,902,831 - +0.7 3,902,836 + +0.1 3,902,836 + -0.4 3,902,837 - +0.5 3,903,184 - -0.5 3,903,184 - -0.4 3,903,184 - -1.6 3,903,184 - +0.9 3,903,214 + -0.2 3,903,214 + -1.9 3,903,215 - +0.8 3,903,215 - -2.0 3,903,228 + Psyr_3260 0.10 -1.6 3,903,229 - Psyr_3260 0.10 +0.2 3,903,229 - Psyr_3260 0.10 -0.3
Or see this region's nucleotide sequence