Experiment: KB with Novobiocin 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3220 and Psyr_3221 are separated by 669 nucleotides Psyr_3221 and Psyr_3222 are separated by 5 nucleotides
Psyr_3220: Psyr_3220 - Aminotransferase, class V, at 3,856,593 to 3,857,783
_3220
Psyr_3221: Psyr_3221 - DegT/DnrJ/EryC1/StrS aminotransferase, at 3,858,453 to 3,859,574
_3221
Psyr_3222: Psyr_3222 - Polysaccharide biosynthesis protein, at 3,859,580 to 3,861,094
_3222
Position (kb)
3858
3859
3860 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3857.472 kb on - strand, within Psyr_3220 at 3857.765 kb on - strand at 3857.765 kb on - strand at 3857.765 kb on - strand at 3857.951 kb on + strand at 3857.956 kb on - strand at 3857.973 kb on - strand at 3857.985 kb on + strand at 3858.026 kb on - strand at 3858.084 kb on + strand at 3858.086 kb on + strand at 3858.086 kb on + strand at 3858.086 kb on + strand at 3858.086 kb on + strand at 3858.086 kb on + strand at 3858.086 kb on + strand at 3858.086 kb on + strand at 3858.086 kb on + strand at 3858.087 kb on - strand at 3858.087 kb on - strand at 3858.087 kb on - strand at 3858.087 kb on - strand at 3858.087 kb on - strand at 3858.088 kb on + strand at 3858.088 kb on + strand at 3858.088 kb on + strand at 3858.088 kb on + strand at 3858.089 kb on - strand at 3858.089 kb on - strand at 3858.089 kb on - strand at 3858.089 kb on - strand at 3858.089 kb on - strand at 3858.089 kb on - strand at 3858.089 kb on - strand at 3858.089 kb on - strand at 3858.114 kb on + strand at 3858.115 kb on - strand at 3858.188 kb on + strand at 3858.188 kb on + strand at 3858.385 kb on + strand at 3858.385 kb on + strand at 3858.386 kb on - strand at 3858.386 kb on - strand at 3858.474 kb on + strand at 3858.474 kb on + strand at 3858.475 kb on - strand at 3858.475 kb on - strand at 3858.475 kb on - strand at 3858.475 kb on - strand at 3858.519 kb on + strand at 3858.519 kb on + strand at 3858.519 kb on + strand at 3858.519 kb on + strand at 3858.520 kb on - strand at 3858.520 kb on - strand at 3858.585 kb on + strand, within Psyr_3221 at 3858.586 kb on - strand, within Psyr_3221 at 3858.606 kb on - strand, within Psyr_3221 at 3858.904 kb on - strand, within Psyr_3221 at 3859.213 kb on - strand, within Psyr_3221 at 3859.213 kb on - strand, within Psyr_3221 at 3859.213 kb on - strand, within Psyr_3221 at 3859.213 kb on - strand, within Psyr_3221 at 3859.281 kb on + strand, within Psyr_3221 at 3859.281 kb on + strand, within Psyr_3221 at 3859.282 kb on - strand, within Psyr_3221 at 3859.282 kb on - strand, within Psyr_3221 at 3859.282 kb on - strand, within Psyr_3221 at 3859.282 kb on - strand, within Psyr_3221 at 3859.366 kb on - strand, within Psyr_3221 at 3859.372 kb on - strand, within Psyr_3221 at 3859.637 kb on + strand at 3859.637 kb on + strand at 3859.637 kb on + strand at 3859.637 kb on + strand at 3859.637 kb on + strand at 3859.637 kb on + strand at 3859.638 kb on - strand at 3859.638 kb on - strand at 3859.638 kb on - strand at 3859.638 kb on - strand at 3859.638 kb on - strand at 3859.638 kb on - strand at 3859.820 kb on + strand, within Psyr_3222 at 3859.821 kb on - strand, within Psyr_3222 at 3859.942 kb on - strand, within Psyr_3222 at 3859.942 kb on - strand, within Psyr_3222 at 3860.003 kb on + strand, within Psyr_3222 at 3860.016 kb on - strand, within Psyr_3222 at 3860.016 kb on - strand, within Psyr_3222 at 3860.558 kb on + strand, within Psyr_3222 at 3860.559 kb on - strand, within Psyr_3222
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 7.75 ug/ml remove 3,857,472 - Psyr_3220 0.74 +0.3 3,857,765 - -0.3 3,857,765 - -0.3 3,857,765 - +0.5 3,857,951 + -1.2 3,857,956 - -1.9 3,857,973 - +0.3 3,857,985 + -2.6 3,858,026 - -2.1 3,858,084 + -3.4 3,858,086 + -2.3 3,858,086 + -0.4 3,858,086 + -0.5 3,858,086 + +0.0 3,858,086 + -3.2 3,858,086 + -1.3 3,858,086 + -2.5 3,858,086 + -2.1 3,858,087 - +0.6 3,858,087 - -3.4 3,858,087 - -2.5 3,858,087 - +0.6 3,858,087 - +0.2 3,858,088 + -0.7 3,858,088 + +0.2 3,858,088 + -3.4 3,858,088 + +0.8 3,858,089 - -2.2 3,858,089 - +1.0 3,858,089 - -1.4 3,858,089 - -2.9 3,858,089 - -0.7 3,858,089 - -1.5 3,858,089 - -0.4 3,858,089 - -1.1 3,858,114 + +0.7 3,858,115 - +0.6 3,858,188 + -0.0 3,858,188 + -1.6 3,858,385 + -1.2 3,858,385 + +1.0 3,858,386 - -1.4 3,858,386 - -1.5 3,858,474 + -0.9 3,858,474 + -0.2 3,858,475 - -0.2 3,858,475 - +1.8 3,858,475 - -2.7 3,858,475 - -1.3 3,858,519 + +0.2 3,858,519 + -1.5 3,858,519 + +2.3 3,858,519 + -1.1 3,858,520 - +1.2 3,858,520 - +0.8 3,858,585 + Psyr_3221 0.12 -1.0 3,858,586 - Psyr_3221 0.12 -1.2 3,858,606 - Psyr_3221 0.14 -2.0 3,858,904 - Psyr_3221 0.40 -1.2 3,859,213 - Psyr_3221 0.68 +0.5 3,859,213 - Psyr_3221 0.68 -0.3 3,859,213 - Psyr_3221 0.68 +0.5 3,859,213 - Psyr_3221 0.68 +0.2 3,859,281 + Psyr_3221 0.74 +0.5 3,859,281 + Psyr_3221 0.74 -0.7 3,859,282 - Psyr_3221 0.74 +0.5 3,859,282 - Psyr_3221 0.74 -1.9 3,859,282 - Psyr_3221 0.74 -0.1 3,859,282 - Psyr_3221 0.74 -2.1 3,859,366 - Psyr_3221 0.81 -0.4 3,859,372 - Psyr_3221 0.82 -2.4 3,859,637 + -0.4 3,859,637 + +0.6 3,859,637 + -2.0 3,859,637 + -0.2 3,859,637 + -0.1 3,859,637 + +0.5 3,859,638 - +0.8 3,859,638 - -0.1 3,859,638 - -0.1 3,859,638 - +1.5 3,859,638 - +0.2 3,859,638 - +0.4 3,859,820 + Psyr_3222 0.16 -0.6 3,859,821 - Psyr_3222 0.16 -2.7 3,859,942 - Psyr_3222 0.24 -1.2 3,859,942 - Psyr_3222 0.24 -0.5 3,860,003 + Psyr_3222 0.28 +0.3 3,860,016 - Psyr_3222 0.29 +0.6 3,860,016 - Psyr_3222 0.29 +0.2 3,860,558 + Psyr_3222 0.65 +0.3 3,860,559 - Psyr_3222 0.65 -2.9
Or see this region's nucleotide sequence