Experiment: KB with Methanol 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2253 and Psyr_2254 overlap by 4 nucleotides Psyr_2254 and Psyr_2255 overlap by 4 nucleotides Psyr_2255 and Psyr_2256 are separated by 41 nucleotides
Psyr_2253: Psyr_2253 - Phosphonate metabolism, at 2,618,095 to 2,619,174
_2253
Psyr_2254: Psyr_2254 - Phosphonate metabolism PhnJ, at 2,619,171 to 2,620,058
_2254
Psyr_2255: Psyr_2255 - ABC transporter, at 2,620,055 to 2,620,876
_2255
Psyr_2256: Psyr_2256 - ABC transporter, at 2,620,918 to 2,621,634
_2256
Position (kb)
2619
2620
2621 Strain fitness (log2 ratio)
-1
0
1 at 2618.257 kb on + strand, within Psyr_2253 at 2618.464 kb on + strand, within Psyr_2253 at 2618.465 kb on - strand, within Psyr_2253 at 2619.098 kb on - strand at 2619.226 kb on - strand at 2619.343 kb on - strand, within Psyr_2254 at 2619.343 kb on - strand, within Psyr_2254 at 2619.573 kb on + strand, within Psyr_2254 at 2619.573 kb on + strand, within Psyr_2254 at 2619.718 kb on - strand, within Psyr_2254 at 2619.718 kb on - strand, within Psyr_2254 at 2619.747 kb on + strand, within Psyr_2254 at 2619.747 kb on + strand, within Psyr_2254 at 2619.747 kb on + strand, within Psyr_2254 at 2619.748 kb on - strand, within Psyr_2254 at 2619.748 kb on - strand, within Psyr_2254 at 2619.748 kb on - strand, within Psyr_2254 at 2619.847 kb on - strand, within Psyr_2254 at 2619.863 kb on + strand, within Psyr_2254 at 2620.126 kb on - strand at 2620.188 kb on - strand, within Psyr_2255 at 2620.469 kb on + strand, within Psyr_2255 at 2620.470 kb on - strand, within Psyr_2255 at 2620.714 kb on - strand, within Psyr_2255 at 2620.714 kb on - strand, within Psyr_2255 at 2620.899 kb on + strand at 2620.983 kb on - strand at 2620.994 kb on + strand, within Psyr_2256
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Methanol 5 vol% remove 2,618,257 + Psyr_2253 0.15 -1.0 2,618,464 + Psyr_2253 0.34 -0.1 2,618,465 - Psyr_2253 0.34 +0.2 2,619,098 - -1.0 2,619,226 - -0.8 2,619,343 - Psyr_2254 0.19 +0.1 2,619,343 - Psyr_2254 0.19 +1.6 2,619,573 + Psyr_2254 0.45 -0.7 2,619,573 + Psyr_2254 0.45 -1.4 2,619,718 - Psyr_2254 0.62 -0.7 2,619,718 - Psyr_2254 0.62 +0.6 2,619,747 + Psyr_2254 0.65 -0.7 2,619,747 + Psyr_2254 0.65 -0.2 2,619,747 + Psyr_2254 0.65 +0.5 2,619,748 - Psyr_2254 0.65 -1.6 2,619,748 - Psyr_2254 0.65 -1.4 2,619,748 - Psyr_2254 0.65 -1.4 2,619,847 - Psyr_2254 0.76 -0.4 2,619,863 + Psyr_2254 0.78 -0.4 2,620,126 - -0.6 2,620,188 - Psyr_2255 0.16 -0.7 2,620,469 + Psyr_2255 0.50 -0.1 2,620,470 - Psyr_2255 0.50 -0.7 2,620,714 - Psyr_2255 0.80 +0.6 2,620,714 - Psyr_2255 0.80 -0.7 2,620,899 + +1.0 2,620,983 - +1.0 2,620,994 + Psyr_2256 0.11 +1.0
Or see this region's nucleotide sequence