Experiment: KB with Methanol 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2167 and Psyr_2168 are separated by 3 nucleotides Psyr_2168 and Psyr_2169 overlap by 20 nucleotides Psyr_2169 and Psyr_RNA40 are separated by 88 nucleotides Psyr_RNA40 and Psyr_2170 are separated by 158 nucleotides Psyr_2170 and Psyr_2171 are separated by 373 nucleotides
Psyr_2167: Psyr_2167 - phenylalanyl-tRNA synthetase beta subunit, at 2,519,998 to 2,522,376
_2167
Psyr_2168: Psyr_2168 - Integration host factor, alpha subunit, at 2,522,380 to 2,522,682
_2168
Psyr_2169: Psyr_2169 - transcriptional regulator, MerR family, at 2,522,663 to 2,523,019
_2169
Psyr_RNA40: Psyr_RNA40 - tRNA-Pro, at 2,523,108 to 2,523,184
_RNA40
Psyr_2170: Psyr_2170 - L-glutamate-binding protein / L-aspartate-binding protein, at 2,523,343 to 2,524,257
_2170
Psyr_2171: Psyr_2171 - HpcH/HpaI aldolase, at 2,524,631 to 2,525,446
_2171
Position (kb)
2523
2524
2525 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2522.420 kb on - strand, within Psyr_2168 at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.662 kb on + strand at 2522.663 kb on - strand at 2522.663 kb on - strand at 2522.663 kb on - strand at 2522.663 kb on - strand at 2522.663 kb on - strand at 2522.714 kb on + strand, within Psyr_2169 at 2522.714 kb on + strand, within Psyr_2169 at 2522.715 kb on - strand, within Psyr_2169 at 2522.715 kb on - strand, within Psyr_2169 at 2522.828 kb on + strand, within Psyr_2169 at 2522.877 kb on + strand, within Psyr_2169 at 2522.877 kb on + strand, within Psyr_2169 at 2522.879 kb on + strand, within Psyr_2169 at 2522.879 kb on + strand, within Psyr_2169 at 2522.880 kb on - strand, within Psyr_2169 at 2522.880 kb on - strand, within Psyr_2169 at 2522.920 kb on + strand, within Psyr_2169 at 2522.955 kb on - strand, within Psyr_2169 at 2522.955 kb on - strand, within Psyr_2169 at 2522.998 kb on - strand at 2523.017 kb on + strand at 2523.017 kb on + strand at 2523.034 kb on + strand at 2523.060 kb on + strand at 2523.063 kb on - strand at 2523.097 kb on - strand at 2523.136 kb on + strand, within Psyr_RNA40 at 2523.137 kb on - strand, within Psyr_RNA40 at 2523.185 kb on + strand at 2523.188 kb on - strand at 2523.210 kb on + strand at 2523.288 kb on - strand at 2523.453 kb on + strand, within Psyr_2170 at 2523.453 kb on + strand, within Psyr_2170 at 2523.462 kb on + strand, within Psyr_2170 at 2523.463 kb on - strand, within Psyr_2170 at 2523.467 kb on + strand, within Psyr_2170 at 2523.467 kb on + strand, within Psyr_2170 at 2523.468 kb on - strand, within Psyr_2170 at 2523.468 kb on - strand, within Psyr_2170 at 2523.505 kb on - strand, within Psyr_2170 at 2523.563 kb on + strand, within Psyr_2170 at 2523.563 kb on + strand, within Psyr_2170 at 2523.658 kb on - strand, within Psyr_2170 at 2523.658 kb on - strand, within Psyr_2170 at 2523.721 kb on + strand, within Psyr_2170 at 2523.721 kb on + strand, within Psyr_2170 at 2523.721 kb on + strand, within Psyr_2170 at 2523.790 kb on - strand, within Psyr_2170 at 2523.796 kb on + strand, within Psyr_2170 at 2523.796 kb on + strand, within Psyr_2170 at 2523.995 kb on + strand, within Psyr_2170 at 2524.000 kb on + strand, within Psyr_2170 at 2524.000 kb on + strand, within Psyr_2170 at 2524.051 kb on + strand, within Psyr_2170 at 2524.052 kb on - strand, within Psyr_2170 at 2524.101 kb on + strand, within Psyr_2170 at 2524.102 kb on - strand, within Psyr_2170 at 2524.102 kb on - strand, within Psyr_2170 at 2524.102 kb on - strand, within Psyr_2170 at 2524.103 kb on + strand, within Psyr_2170 at 2524.103 kb on + strand, within Psyr_2170 at 2524.103 kb on + strand, within Psyr_2170 at 2524.103 kb on + strand, within Psyr_2170 at 2524.103 kb on + strand, within Psyr_2170 at 2524.103 kb on + strand, within Psyr_2170 at 2524.103 kb on + strand, within Psyr_2170 at 2524.104 kb on - strand, within Psyr_2170 at 2524.104 kb on - strand, within Psyr_2170 at 2524.104 kb on - strand, within Psyr_2170 at 2524.104 kb on - strand, within Psyr_2170 at 2524.104 kb on - strand, within Psyr_2170 at 2524.104 kb on - strand, within Psyr_2170 at 2524.104 kb on - strand, within Psyr_2170 at 2524.104 kb on - strand, within Psyr_2170 at 2524.204 kb on - strand at 2524.204 kb on - strand at 2524.206 kb on + strand at 2524.206 kb on + strand at 2524.206 kb on + strand at 2524.206 kb on + strand at 2524.206 kb on + strand at 2524.207 kb on - strand at 2524.207 kb on - strand at 2524.207 kb on - strand at 2524.207 kb on - strand at 2524.207 kb on - strand at 2524.207 kb on - strand at 2524.207 kb on - strand at 2524.207 kb on - strand at 2524.207 kb on - strand at 2524.210 kb on + strand at 2524.210 kb on + strand at 2524.251 kb on + strand at 2524.252 kb on - strand at 2524.252 kb on - strand at 2524.252 kb on - strand at 2524.272 kb on + strand at 2524.272 kb on + strand at 2524.273 kb on - strand at 2524.350 kb on + strand at 2524.354 kb on + strand at 2524.442 kb on + strand at 2524.456 kb on + strand at 2524.457 kb on - strand at 2524.548 kb on + strand at 2524.549 kb on - strand at 2524.601 kb on + strand at 2524.601 kb on + strand at 2524.601 kb on + strand at 2524.601 kb on + strand at 2524.602 kb on - strand at 2524.602 kb on - strand at 2524.602 kb on - strand at 2524.602 kb on - strand at 2524.608 kb on + strand at 2524.609 kb on - strand at 2524.863 kb on - strand, within Psyr_2171 at 2524.863 kb on - strand, within Psyr_2171 at 2524.866 kb on - strand, within Psyr_2171 at 2524.895 kb on + strand, within Psyr_2171 at 2524.896 kb on - strand, within Psyr_2171
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Methanol 5 vol% remove 2,522,420 - Psyr_2168 0.13 -0.3 2,522,662 + -0.3 2,522,662 + -0.1 2,522,662 + -1.7 2,522,662 + -0.6 2,522,662 + +4.5 2,522,663 - -0.8 2,522,663 - -1.4 2,522,663 - -1.3 2,522,663 - +1.5 2,522,663 - -3.3 2,522,714 + Psyr_2169 0.14 +1.2 2,522,714 + Psyr_2169 0.14 +0.1 2,522,715 - Psyr_2169 0.15 -1.2 2,522,715 - Psyr_2169 0.15 -0.1 2,522,828 + Psyr_2169 0.46 -1.3 2,522,877 + Psyr_2169 0.60 -0.8 2,522,877 + Psyr_2169 0.60 -0.8 2,522,879 + Psyr_2169 0.61 +0.7 2,522,879 + Psyr_2169 0.61 +0.7 2,522,880 - Psyr_2169 0.61 +0.9 2,522,880 - Psyr_2169 0.61 -0.8 2,522,920 + Psyr_2169 0.72 -0.3 2,522,955 - Psyr_2169 0.82 +0.7 2,522,955 - Psyr_2169 0.82 +0.5 2,522,998 - +0.4 2,523,017 + +0.7 2,523,017 + +1.2 2,523,034 + +0.3 2,523,060 + -0.4 2,523,063 - +0.3 2,523,097 - -0.6 2,523,136 + Psyr_RNA40 0.36 +0.1 2,523,137 - Psyr_RNA40 0.38 -1.2 2,523,185 + +0.7 2,523,188 - -1.2 2,523,210 + +1.4 2,523,288 - -1.0 2,523,453 + Psyr_2170 0.12 -1.2 2,523,453 + Psyr_2170 0.12 +0.7 2,523,462 + Psyr_2170 0.13 -2.2 2,523,463 - Psyr_2170 0.13 -1.0 2,523,467 + Psyr_2170 0.14 -0.4 2,523,467 + Psyr_2170 0.14 -1.2 2,523,468 - Psyr_2170 0.14 -0.9 2,523,468 - Psyr_2170 0.14 -0.3 2,523,505 - Psyr_2170 0.18 -1.4 2,523,563 + Psyr_2170 0.24 +0.5 2,523,563 + Psyr_2170 0.24 -1.7 2,523,658 - Psyr_2170 0.34 -0.7 2,523,658 - Psyr_2170 0.34 -0.3 2,523,721 + Psyr_2170 0.41 -0.3 2,523,721 + Psyr_2170 0.41 -0.3 2,523,721 + Psyr_2170 0.41 -0.3 2,523,790 - Psyr_2170 0.49 -1.3 2,523,796 + Psyr_2170 0.50 -0.2 2,523,796 + Psyr_2170 0.50 -0.1 2,523,995 + Psyr_2170 0.71 +0.4 2,524,000 + Psyr_2170 0.72 +0.5 2,524,000 + Psyr_2170 0.72 +0.2 2,524,051 + Psyr_2170 0.77 +1.0 2,524,052 - Psyr_2170 0.77 +1.2 2,524,101 + Psyr_2170 0.83 +0.7 2,524,102 - Psyr_2170 0.83 +0.5 2,524,102 - Psyr_2170 0.83 +0.1 2,524,102 - Psyr_2170 0.83 -0.2 2,524,103 + Psyr_2170 0.83 -0.5 2,524,103 + Psyr_2170 0.83 -0.2 2,524,103 + Psyr_2170 0.83 +0.8 2,524,103 + Psyr_2170 0.83 +0.9 2,524,103 + Psyr_2170 0.83 +0.9 2,524,103 + Psyr_2170 0.83 +0.1 2,524,103 + Psyr_2170 0.83 +0.7 2,524,104 - Psyr_2170 0.83 +0.4 2,524,104 - Psyr_2170 0.83 -0.1 2,524,104 - Psyr_2170 0.83 -0.7 2,524,104 - Psyr_2170 0.83 -1.8 2,524,104 - Psyr_2170 0.83 -0.3 2,524,104 - Psyr_2170 0.83 -0.2 2,524,104 - Psyr_2170 0.83 -1.9 2,524,104 - Psyr_2170 0.83 -1.0 2,524,204 - +0.2 2,524,204 - -1.7 2,524,206 + -0.3 2,524,206 + -0.3 2,524,206 + -0.5 2,524,206 + +1.5 2,524,206 + +0.3 2,524,207 - -0.2 2,524,207 - +1.2 2,524,207 - -0.9 2,524,207 - -2.1 2,524,207 - +0.1 2,524,207 - -0.5 2,524,207 - +0.8 2,524,207 - -1.5 2,524,207 - +0.2 2,524,210 + -0.4 2,524,210 + -0.1 2,524,251 + -0.9 2,524,252 - +0.1 2,524,252 - +0.7 2,524,252 - -0.0 2,524,272 + -0.5 2,524,272 + -0.6 2,524,273 - +1.2 2,524,350 + -0.2 2,524,354 + +0.9 2,524,442 + -0.2 2,524,456 + -0.2 2,524,457 - +0.2 2,524,548 + -0.2 2,524,549 - +0.8 2,524,601 + +0.2 2,524,601 + +0.1 2,524,601 + -1.5 2,524,601 + +0.2 2,524,602 - -0.8 2,524,602 - +2.1 2,524,602 - +0.6 2,524,602 - -2.1 2,524,608 + -0.4 2,524,609 - +0.5 2,524,863 - Psyr_2171 0.28 +0.3 2,524,863 - Psyr_2171 0.28 +0.4 2,524,866 - Psyr_2171 0.29 +0.5 2,524,895 + Psyr_2171 0.32 +0.3 2,524,896 - Psyr_2171 0.32 +1.0
Or see this region's nucleotide sequence