Experiment: KB with Methanol 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0358 and Psyr_0359 are separated by 219 nucleotides Psyr_0359 and Psyr_0360 are separated by 197 nucleotides
Psyr_0358: Psyr_0358 - Diaminopimelate-binding protein / L-cystine-binding protein, at 383,399 to 384,196
_0358
Psyr_0359: Psyr_0359 - D-cysteine desulfhydrase, at 384,416 to 385,414
_0359
Psyr_0360: Psyr_0360 - serine O-acetyltransferase, at 385,612 to 386,565
_0360
Position (kb) 384
385
386 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 383.424 kb on + strand at 383.636 kb on + strand, within Psyr_0358 at 383.636 kb on + strand, within Psyr_0358 at 383.636 kb on + strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.673 kb on + strand, within Psyr_0358 at 383.673 kb on + strand, within Psyr_0358 at 383.674 kb on - strand, within Psyr_0358 at 383.674 kb on - strand, within Psyr_0358 at 383.721 kb on + strand, within Psyr_0358 at 383.721 kb on + strand, within Psyr_0358 at 383.721 kb on + strand, within Psyr_0358 at 383.722 kb on - strand, within Psyr_0358 at 383.722 kb on - strand, within Psyr_0358 at 383.829 kb on + strand, within Psyr_0358 at 383.829 kb on + strand, within Psyr_0358 at 383.829 kb on + strand, within Psyr_0358 at 383.829 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 384.048 kb on + strand, within Psyr_0358 at 384.048 kb on + strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.173 kb on - strand at 384.265 kb on + strand at 384.287 kb on + strand at 384.287 kb on + strand at 384.287 kb on + strand at 384.288 kb on - strand at 384.288 kb on - strand at 384.298 kb on + strand at 384.298 kb on + strand at 384.298 kb on + strand at 384.299 kb on - strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.332 kb on - strand at 384.332 kb on - strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.334 kb on - strand at 384.334 kb on - strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.336 kb on - strand at 384.336 kb on - strand at 384.348 kb on - strand at 384.546 kb on + strand, within Psyr_0359 at 384.639 kb on + strand, within Psyr_0359 at 384.639 kb on + strand, within Psyr_0359 at 384.639 kb on + strand, within Psyr_0359 at 384.640 kb on - strand, within Psyr_0359 at 384.909 kb on + strand, within Psyr_0359 at 384.909 kb on + strand, within Psyr_0359 at 384.910 kb on - strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.038 kb on - strand, within Psyr_0359 at 385.087 kb on - strand, within Psyr_0359 at 385.210 kb on + strand, within Psyr_0359 at 385.245 kb on + strand, within Psyr_0359 at 385.246 kb on - strand, within Psyr_0359 at 385.246 kb on - strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.424 kb on + strand at 385.424 kb on + strand at 385.424 kb on + strand at 385.434 kb on - strand at 385.449 kb on + strand at 385.449 kb on + strand at 385.449 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.548 kb on + strand at 385.549 kb on - strand at 385.556 kb on + strand at 385.559 kb on + strand at 385.559 kb on + strand at 385.560 kb on - strand at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.869 kb on + strand, within Psyr_0360 at 385.870 kb on - strand, within Psyr_0360 at 385.870 kb on - strand, within Psyr_0360 at 385.870 kb on - strand, within Psyr_0360 at 385.925 kb on - strand, within Psyr_0360 at 386.140 kb on + strand, within Psyr_0360 at 386.140 kb on + strand, within Psyr_0360 at 386.141 kb on - strand, within Psyr_0360 at 386.141 kb on - strand, within Psyr_0360 at 386.141 kb on - strand, within Psyr_0360 at 386.141 kb on - strand, within Psyr_0360 at 386.141 kb on - strand, within Psyr_0360 at 386.146 kb on + strand, within Psyr_0360 at 386.175 kb on + strand, within Psyr_0360 at 386.176 kb on - strand, within Psyr_0360 at 386.214 kb on + strand, within Psyr_0360 at 386.314 kb on + strand, within Psyr_0360 at 386.314 kb on + strand, within Psyr_0360 at 386.314 kb on + strand, within Psyr_0360 at 386.315 kb on - strand, within Psyr_0360
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Methanol 5 vol% remove 383,424 + +1.1 383,636 + Psyr_0358 0.30 -0.7 383,636 + Psyr_0358 0.30 -0.3 383,636 + Psyr_0358 0.30 -2.8 383,637 - Psyr_0358 0.30 -0.6 383,637 - Psyr_0358 0.30 +0.1 383,637 - Psyr_0358 0.30 -0.7 383,637 - Psyr_0358 0.30 +0.2 383,637 - Psyr_0358 0.30 -0.7 383,637 - Psyr_0358 0.30 -0.7 383,673 + Psyr_0358 0.34 -0.5 383,673 + Psyr_0358 0.34 +1.9 383,674 - Psyr_0358 0.34 -0.2 383,674 - Psyr_0358 0.34 -0.6 383,721 + Psyr_0358 0.40 -0.4 383,721 + Psyr_0358 0.40 +0.9 383,721 + Psyr_0358 0.40 -0.7 383,722 - Psyr_0358 0.40 -0.4 383,722 - Psyr_0358 0.40 +0.5 383,829 + Psyr_0358 0.54 +0.2 383,829 + Psyr_0358 0.54 +0.6 383,829 + Psyr_0358 0.54 +0.3 383,829 + Psyr_0358 0.54 +3.1 383,847 + Psyr_0358 0.56 +0.5 383,847 + Psyr_0358 0.56 -1.4 383,847 + Psyr_0358 0.56 -0.5 383,847 + Psyr_0358 0.56 -0.2 384,048 + Psyr_0358 0.81 +0.3 384,048 + Psyr_0358 0.81 -1.6 384,049 - Psyr_0358 0.81 +0.3 384,049 - Psyr_0358 0.81 -0.3 384,049 - Psyr_0358 0.81 +0.3 384,049 - Psyr_0358 0.81 +1.9 384,173 - +0.5 384,265 + +0.5 384,287 + +1.0 384,287 + -0.1 384,287 + -0.2 384,288 - +0.4 384,288 - -0.8 384,298 + -0.3 384,298 + -0.5 384,298 + +1.3 384,299 - -0.1 384,331 + +0.3 384,331 + -0.1 384,331 + +0.2 384,332 - -0.4 384,332 - +1.0 384,333 + +1.1 384,333 + -0.4 384,333 + -0.6 384,333 + +0.5 384,333 + +0.2 384,333 + +0.9 384,333 + -0.1 384,333 + -0.3 384,333 + +0.0 384,334 - -0.8 384,334 - -0.8 384,335 + +0.0 384,335 + -0.7 384,335 + -0.1 384,335 + +0.2 384,336 - +1.5 384,336 - -1.9 384,348 - +0.7 384,546 + Psyr_0359 0.13 -0.2 384,639 + Psyr_0359 0.22 -1.7 384,639 + Psyr_0359 0.22 +0.4 384,639 + Psyr_0359 0.22 +1.1 384,640 - Psyr_0359 0.22 +0.2 384,909 + Psyr_0359 0.49 -0.5 384,909 + Psyr_0359 0.49 +0.3 384,910 - Psyr_0359 0.49 -0.4 385,037 + Psyr_0359 0.62 +0.7 385,037 + Psyr_0359 0.62 +0.3 385,037 + Psyr_0359 0.62 +0.8 385,037 + Psyr_0359 0.62 +0.4 385,037 + Psyr_0359 0.62 +0.4 385,038 - Psyr_0359 0.62 +0.4 385,087 - Psyr_0359 0.67 -0.5 385,210 + Psyr_0359 0.79 -0.6 385,245 + Psyr_0359 0.83 -1.0 385,246 - Psyr_0359 0.83 +0.3 385,246 - Psyr_0359 0.83 -0.8 385,308 + Psyr_0359 0.89 +0.2 385,308 + Psyr_0359 0.89 +0.1 385,308 + Psyr_0359 0.89 +0.6 385,308 + Psyr_0359 0.89 -0.1 385,308 + Psyr_0359 0.89 -0.8 385,308 + Psyr_0359 0.89 +0.4 385,308 + Psyr_0359 0.89 +0.2 385,308 + Psyr_0359 0.89 +0.7 385,308 + Psyr_0359 0.89 +0.9 385,309 - Psyr_0359 0.89 -0.6 385,309 - Psyr_0359 0.89 -1.1 385,309 - Psyr_0359 0.89 +0.2 385,309 - Psyr_0359 0.89 +0.9 385,309 - Psyr_0359 0.89 +0.4 385,309 - Psyr_0359 0.89 -0.6 385,309 - Psyr_0359 0.89 +1.6 385,309 - Psyr_0359 0.89 -1.8 385,309 - Psyr_0359 0.89 -1.5 385,309 - Psyr_0359 0.89 -0.5 385,309 - Psyr_0359 0.89 -2.1 385,424 + -0.1 385,424 + -0.5 385,424 + -0.2 385,434 - -0.6 385,449 + +0.6 385,449 + +0.1 385,449 + -0.5 385,478 + -0.4 385,478 + +0.8 385,478 + -2.3 385,478 + +0.5 385,478 + +0.1 385,478 + +0.0 385,478 + -0.2 385,478 + +1.0 385,478 + +0.7 385,479 - +0.6 385,479 - -1.4 385,479 - -0.7 385,479 - -0.4 385,479 - +0.9 385,479 - -0.0 385,479 - -1.0 385,548 + +0.4 385,549 - +1.0 385,556 + -1.1 385,559 + +0.1 385,559 + -0.1 385,560 - +0.4 385,858 + Psyr_0360 0.26 +0.1 385,858 + Psyr_0360 0.26 -1.7 385,858 + Psyr_0360 0.26 +0.8 385,858 + Psyr_0360 0.26 +0.1 385,858 + Psyr_0360 0.26 +0.4 385,858 + Psyr_0360 0.26 +0.4 385,858 + Psyr_0360 0.26 +0.1 385,858 + Psyr_0360 0.26 -1.8 385,858 + Psyr_0360 0.26 +1.1 385,858 + Psyr_0360 0.26 -0.0 385,859 - Psyr_0360 0.26 -0.7 385,859 - Psyr_0360 0.26 +0.7 385,859 - Psyr_0360 0.26 -0.1 385,859 - Psyr_0360 0.26 -0.7 385,859 - Psyr_0360 0.26 -0.8 385,859 - Psyr_0360 0.26 -0.1 385,859 - Psyr_0360 0.26 +1.3 385,859 - Psyr_0360 0.26 +0.2 385,859 - Psyr_0360 0.26 +0.3 385,869 + Psyr_0360 0.27 -0.1 385,870 - Psyr_0360 0.27 -1.3 385,870 - Psyr_0360 0.27 -0.9 385,870 - Psyr_0360 0.27 +0.1 385,925 - Psyr_0360 0.33 +0.1 386,140 + Psyr_0360 0.55 +0.9 386,140 + Psyr_0360 0.55 -0.2 386,141 - Psyr_0360 0.55 +1.0 386,141 - Psyr_0360 0.55 -0.5 386,141 - Psyr_0360 0.55 -0.4 386,141 - Psyr_0360 0.55 -2.0 386,141 - Psyr_0360 0.55 -0.6 386,146 + Psyr_0360 0.56 +1.0 386,175 + Psyr_0360 0.59 +0.3 386,176 - Psyr_0360 0.59 -0.1 386,214 + Psyr_0360 0.63 +0.1 386,314 + Psyr_0360 0.74 -0.2 386,314 + Psyr_0360 0.74 +1.6 386,314 + Psyr_0360 0.74 -0.6 386,315 - Psyr_0360 0.74 +0.3
Or see this region's nucleotide sequence