Experiment: 3-hydroxypentanoate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0772 and PP_0773 are separated by 118 nucleotides PP_0773 and PP_0774 are separated by 79 nucleotides PP_0774 and PP_0775 are separated by 31 nucleotides PP_0775 and PP_0776 overlap by 2 nucleotides PP_0776 and PP_0777 are separated by 145 nucleotides
PP_0772: PP_0772 - Metallo-beta-lactamase family protein, at 890,074 to 890,718
_0772
PP_0773: PP_0773 - OmpA/MotB domain-containing protein, at 890,837 to 891,547
_0773
PP_0774: PP_0774 - Phosphate acetyltransferase, at 891,627 to 893,714
_0774
PP_0775: PP_0775 - conserved protein of unknown function, at 893,746 to 894,048
_0775
PP_0776: PP_0776 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 894,047 to 894,532
_0776
PP_0777: PP_0777 - Glutathione peroxidase, at 894,678 to 895,160
_0777
Position (kb) 891
892
893
894 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.816 kb on - strand at 890.903 kb on + strand at 890.904 kb on - strand at 890.957 kb on + strand, within PP_0773 at 891.080 kb on + strand, within PP_0773 at 891.224 kb on + strand, within PP_0773 at 891.365 kb on + strand, within PP_0773 at 891.365 kb on + strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.405 kb on + strand, within PP_0773 at 891.406 kb on - strand, within PP_0773 at 891.512 kb on + strand at 891.512 kb on + strand at 891.512 kb on + strand at 891.513 kb on - strand at 891.513 kb on - strand at 891.540 kb on - strand at 891.555 kb on + strand at 891.556 kb on - strand at 891.556 kb on - strand at 891.663 kb on + strand at 891.664 kb on - strand at 891.668 kb on + strand at 891.668 kb on + strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.771 kb on + strand at 891.788 kb on + strand at 891.789 kb on - strand at 891.893 kb on - strand, within PP_0774 at 891.997 kb on + strand, within PP_0774 at 891.997 kb on + strand, within PP_0774 at 891.997 kb on + strand, within PP_0774 at 891.998 kb on - strand, within PP_0774 at 892.366 kb on + strand, within PP_0774 at 892.756 kb on + strand, within PP_0774 at 892.756 kb on + strand, within PP_0774 at 892.756 kb on + strand, within PP_0774 at 892.757 kb on - strand, within PP_0774 at 892.757 kb on - strand, within PP_0774 at 892.757 kb on - strand, within PP_0774 at 892.757 kb on - strand, within PP_0774 at 892.957 kb on - strand, within PP_0774 at 893.020 kb on + strand, within PP_0774 at 893.192 kb on + strand, within PP_0774 at 893.320 kb on - strand, within PP_0774 at 893.320 kb on - strand, within PP_0774 at 893.320 kb on - strand, within PP_0774 at 893.438 kb on - strand, within PP_0774 at 893.600 kb on + strand at 893.600 kb on + strand at 893.731 kb on - strand at 893.731 kb on - strand at 893.761 kb on - strand at 894.004 kb on + strand, within PP_0775 at 894.004 kb on + strand, within PP_0775 at 894.005 kb on - strand, within PP_0775 at 894.019 kb on + strand at 894.083 kb on + strand at 894.083 kb on + strand at 894.084 kb on - strand at 894.084 kb on - strand at 894.084 kb on - strand at 894.085 kb on + strand at 894.086 kb on - strand at 894.166 kb on + strand, within PP_0776 at 894.248 kb on + strand, within PP_0776 at 894.257 kb on + strand, within PP_0776 at 894.544 kb on - strand at 894.544 kb on - strand at 894.624 kb on + strand at 894.624 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-hydroxypentanoate remove 890,777 + -0.5 890,777 + -0.7 890,777 + +0.2 890,777 + +1.5 890,777 + +0.3 890,777 + +0.0 890,777 + +1.0 890,777 + -0.9 890,778 - +0.4 890,778 - -0.3 890,778 - +0.8 890,778 - -0.2 890,778 - -0.1 890,816 - +0.2 890,903 + +0.3 890,904 - +0.6 890,957 + PP_0773 0.17 +0.3 891,080 + PP_0773 0.34 +0.4 891,224 + PP_0773 0.54 +0.9 891,365 + PP_0773 0.74 -0.1 891,365 + PP_0773 0.74 -0.1 891,366 - PP_0773 0.74 -0.0 891,366 - PP_0773 0.74 +0.0 891,366 - PP_0773 0.74 +0.4 891,366 - PP_0773 0.74 -1.9 891,405 + PP_0773 0.80 +1.2 891,406 - PP_0773 0.80 -0.1 891,512 + -0.3 891,512 + -0.1 891,512 + -0.4 891,513 - +0.6 891,513 - +1.4 891,540 - +1.1 891,555 + -0.3 891,556 - +0.7 891,556 - +0.1 891,663 + -0.2 891,664 - +0.4 891,668 + +0.0 891,668 + +0.3 891,669 - -0.9 891,669 - -0.8 891,669 - -0.0 891,669 - -0.6 891,669 - -0.2 891,669 - +0.5 891,669 - +0.2 891,669 - +0.2 891,771 + +0.5 891,788 + -0.1 891,789 - -0.3 891,893 - PP_0774 0.13 +1.2 891,997 + PP_0774 0.18 -1.2 891,997 + PP_0774 0.18 -0.1 891,997 + PP_0774 0.18 -0.9 891,998 - PP_0774 0.18 -1.1 892,366 + PP_0774 0.35 +0.2 892,756 + PP_0774 0.54 +0.8 892,756 + PP_0774 0.54 -0.5 892,756 + PP_0774 0.54 +0.6 892,757 - PP_0774 0.54 -1.1 892,757 - PP_0774 0.54 +0.2 892,757 - PP_0774 0.54 +0.6 892,757 - PP_0774 0.54 -0.3 892,957 - PP_0774 0.64 +0.5 893,020 + PP_0774 0.67 +0.7 893,192 + PP_0774 0.75 +0.9 893,320 - PP_0774 0.81 -0.1 893,320 - PP_0774 0.81 +0.2 893,320 - PP_0774 0.81 +1.1 893,438 - PP_0774 0.87 -0.3 893,600 + -0.4 893,600 + -0.6 893,731 - -1.0 893,731 - -0.3 893,761 - -0.9 894,004 + PP_0775 0.85 -0.2 894,004 + PP_0775 0.85 -1.9 894,005 - PP_0775 0.85 +1.0 894,019 + +0.6 894,083 + +0.4 894,083 + -0.8 894,084 - -0.6 894,084 - +3.4 894,084 - -0.2 894,085 + +1.0 894,086 - -1.4 894,166 + PP_0776 0.24 -0.5 894,248 + PP_0776 0.41 +1.0 894,257 + PP_0776 0.43 -1.2 894,544 - +0.5 894,544 - -0.6 894,624 + +0.2 894,624 + +0.2
Or see this region's nucleotide sequence